The pathophysiology of bile acid diarrhoea: differences in the colonic microbiome, metabolome and bile acids

Abstract Bile acid diarrhoea (BAD) is a common disorder resulting from increased loss of bile acids (BAs), overlapping irritable bowel syndrome with diarrhoea (IBS-D). The gut microbiota metabolises primary BAs to secondary BAs, with differing impacts on metabolism and homeostasis. The aim of this s...

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Autores principales:	Nidhi M. Sagar, Henri Duboc, Gemma L. Kay, Mohammad T. Alam, Alfian N. Wicaksono, James A. Covington, Christopher Quince, Margarita Kokkorou, Vaios Svolos, Lola J. Palmieri, Konstantinos Gerasimidis, Julian R. F. Walters, Ramesh P. Arasaradnam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1cfc811b99244d6a20d7c655bd6cd31
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spelling	oai:doaj.org-article:a1cfc811b99244d6a20d7c655bd6cd312021-12-02T12:33:45ZThe pathophysiology of bile acid diarrhoea: differences in the colonic microbiome, metabolome and bile acids10.1038/s41598-020-77374-72045-2322https://doaj.org/article/a1cfc811b99244d6a20d7c655bd6cd312020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77374-7https://doaj.org/toc/2045-2322Abstract Bile acid diarrhoea (BAD) is a common disorder resulting from increased loss of bile acids (BAs), overlapping irritable bowel syndrome with diarrhoea (IBS-D). The gut microbiota metabolises primary BAs to secondary BAs, with differing impacts on metabolism and homeostasis. The aim of this study was to profile the microbiome, metabolic products and bile acids in BAD. Patients with BAD diagnosed by SeHCAT testing, were compared with other IBS-D patients, and healthy controls. Faecal 16S ribosomal RNA gene analysis was undertaken. Faecal short chain fatty acid (SCFA) and urinary volatile organic compounds (VOCs) were measured. BAs were quantified in serum and faeces. Faecal bacterial diversity was significantly reduced in patients with BAD. Several taxa were enriched compared to IBS-D. SCFA amounts differed in BAD, controls and IBS-D, with significantly more propionate in BAD. Separation of VOC profiles was evident, but the greatest discrimination was between IBS-D and controls. Unconjugated and primary BA in serum and faeces were significantly higher in BAD. The faecal percentage primary BA was inversely related to SeHCAT. BAD produces dysbiosis, with metabolite differences, including VOC, SCFA and primary BAs when compared to IBS-D. These findings provide new mechanistic insights into the pathophysiology of BAD.Nidhi M. SagarHenri DubocGemma L. KayMohammad T. AlamAlfian N. WicaksonoJames A. CovingtonChristopher QuinceMargarita KokkorouVaios SvolosLola J. PalmieriKonstantinos GerasimidisJulian R. F. WaltersRamesh P. ArasaradnamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nidhi M. Sagar Henri Duboc Gemma L. Kay Mohammad T. Alam Alfian N. Wicaksono James A. Covington Christopher Quince Margarita Kokkorou Vaios Svolos Lola J. Palmieri Konstantinos Gerasimidis Julian R. F. Walters Ramesh P. Arasaradnam The pathophysiology of bile acid diarrhoea: differences in the colonic microbiome, metabolome and bile acids
description	Abstract Bile acid diarrhoea (BAD) is a common disorder resulting from increased loss of bile acids (BAs), overlapping irritable bowel syndrome with diarrhoea (IBS-D). The gut microbiota metabolises primary BAs to secondary BAs, with differing impacts on metabolism and homeostasis. The aim of this study was to profile the microbiome, metabolic products and bile acids in BAD. Patients with BAD diagnosed by SeHCAT testing, were compared with other IBS-D patients, and healthy controls. Faecal 16S ribosomal RNA gene analysis was undertaken. Faecal short chain fatty acid (SCFA) and urinary volatile organic compounds (VOCs) were measured. BAs were quantified in serum and faeces. Faecal bacterial diversity was significantly reduced in patients with BAD. Several taxa were enriched compared to IBS-D. SCFA amounts differed in BAD, controls and IBS-D, with significantly more propionate in BAD. Separation of VOC profiles was evident, but the greatest discrimination was between IBS-D and controls. Unconjugated and primary BA in serum and faeces were significantly higher in BAD. The faecal percentage primary BA was inversely related to SeHCAT. BAD produces dysbiosis, with metabolite differences, including VOC, SCFA and primary BAs when compared to IBS-D. These findings provide new mechanistic insights into the pathophysiology of BAD.
format	article
author	Nidhi M. Sagar Henri Duboc Gemma L. Kay Mohammad T. Alam Alfian N. Wicaksono James A. Covington Christopher Quince Margarita Kokkorou Vaios Svolos Lola J. Palmieri Konstantinos Gerasimidis Julian R. F. Walters Ramesh P. Arasaradnam
author_facet	Nidhi M. Sagar Henri Duboc Gemma L. Kay Mohammad T. Alam Alfian N. Wicaksono James A. Covington Christopher Quince Margarita Kokkorou Vaios Svolos Lola J. Palmieri Konstantinos Gerasimidis Julian R. F. Walters Ramesh P. Arasaradnam
author_sort	Nidhi M. Sagar
title	The pathophysiology of bile acid diarrhoea: differences in the colonic microbiome, metabolome and bile acids
title_short	The pathophysiology of bile acid diarrhoea: differences in the colonic microbiome, metabolome and bile acids
title_full	The pathophysiology of bile acid diarrhoea: differences in the colonic microbiome, metabolome and bile acids
title_fullStr	The pathophysiology of bile acid diarrhoea: differences in the colonic microbiome, metabolome and bile acids
title_full_unstemmed	The pathophysiology of bile acid diarrhoea: differences in the colonic microbiome, metabolome and bile acids
title_sort	pathophysiology of bile acid diarrhoea: differences in the colonic microbiome, metabolome and bile acids
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a1cfc811b99244d6a20d7c655bd6cd31
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The pathophysiology of bile acid diarrhoea: differences in the colonic microbiome, metabolome and bile acids

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