Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa)

Abstract. Inyod I, SassanarakitS, PayapanonA, Keawsompong S. 2017. Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa). Biodiversitas 18: 221-228. Hed Taen Rad or Macrocybe crassa is a wild edible mushroom found i...

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Autores principales:	TANAPAK INYOD, SURIYA SASSANARAKIT, ACHARA PAYAPANON, SUTTIPUN KEAWSOMPONG
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	hed taen rad, macrocybe crassa, molecular analysis, morphological characteristics Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a1d90e5dcdd94d52832cc2b7e2bec3da
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spelling	oai:doaj.org-article:a1d90e5dcdd94d52832cc2b7e2bec3da2021-11-16T13:50:45ZMorphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa)1412-033X2085-472210.13057/biodiv/d180128https://doaj.org/article/a1d90e5dcdd94d52832cc2b7e2bec3da2017-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/771https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Inyod I, SassanarakitS, PayapanonA, Keawsompong S. 2017. Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa). Biodiversitas 18: 221-228. Hed Taen Rad or Macrocybe crassa is a wild edible mushroom found in Thailand. It has a large fruiting body with a meaty texture and a delicious taste. Five strains of Hed Taen Rad; DOA, DOA-1, DOA-4, DOA-7 and DOA-10 were tested in different media, pH values and temperatures to measure mycelium growth kinetics and identify mycelia and basidiomata. Results indicated that mushroom mycelial growth rates were highest on MEA medium at pH values between 8 and 10, and temperatures between 20Â°C and 30Â°C. Identification of the isolates was based on the morphology characteristics of the basidiomata were closely similar to M. crassa (Berk.), and different from M. gigantea (Massee). For molecular analysis by sequencing of their internal transcribed spacer (ITS) regions was identified as a T. giganteum since limited ITS rDNA sequences from M. crassa exist in public databases. Therefore, the identification of five strains of Hed Taen Rad using molecular methods could be erroneous. Therefore, morphology characteristics study is suitable for further analysis method on genetic study of Hed Taen Rad. Keywords: Hed Taen Rad, Macrocybe crassa, molecular analysis, morphological characteristicsTANAPAK INYODSURIYA SASSANARAKITACHARA PAYAPANONSUTTIPUN KEAWSOMPONGMBI & UNS Soloarticlehed taen rad, macrocybe crassa, molecular analysis, morphological characteristicsBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 1 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	hed taen rad, macrocybe crassa, molecular analysis, morphological characteristics Biology (General) QH301-705.5
spellingShingle	hed taen rad, macrocybe crassa, molecular analysis, morphological characteristics Biology (General) QH301-705.5 TANAPAK INYOD SURIYA SASSANARAKIT ACHARA PAYAPANON SUTTIPUN KEAWSOMPONG Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa)
description	Abstract. Inyod I, SassanarakitS, PayapanonA, Keawsompong S. 2017. Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa). Biodiversitas 18: 221-228. Hed Taen Rad or Macrocybe crassa is a wild edible mushroom found in Thailand. It has a large fruiting body with a meaty texture and a delicious taste. Five strains of Hed Taen Rad; DOA, DOA-1, DOA-4, DOA-7 and DOA-10 were tested in different media, pH values and temperatures to measure mycelium growth kinetics and identify mycelia and basidiomata. Results indicated that mushroom mycelial growth rates were highest on MEA medium at pH values between 8 and 10, and temperatures between 20Â°C and 30Â°C. Identification of the isolates was based on the morphology characteristics of the basidiomata were closely similar to M. crassa (Berk.), and different from M. gigantea (Massee). For molecular analysis by sequencing of their internal transcribed spacer (ITS) regions was identified as a T. giganteum since limited ITS rDNA sequences from M. crassa exist in public databases. Therefore, the identification of five strains of Hed Taen Rad using molecular methods could be erroneous. Therefore, morphology characteristics study is suitable for further analysis method on genetic study of Hed Taen Rad. Keywords: Hed Taen Rad, Macrocybe crassa, molecular analysis, morphological characteristics
format	article
author	TANAPAK INYOD SURIYA SASSANARAKIT ACHARA PAYAPANON SUTTIPUN KEAWSOMPONG
author_facet	TANAPAK INYOD SURIYA SASSANARAKIT ACHARA PAYAPANON SUTTIPUN KEAWSOMPONG
author_sort	TANAPAK INYOD
title	Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa)
title_short	Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa)
title_full	Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa)
title_fullStr	Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa)
title_full_unstemmed	Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa)
title_sort	morphological characteristics and molecular identification of a wild thai isolate of the tropical mushroom hed taen rad (macrocybe crassa)
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/a1d90e5dcdd94d52832cc2b7e2bec3da
work_keys_str_mv	AT tanapakinyod morphologicalcharacteristicsandmolecularidentificationofawildthaiisolateofthetropicalmushroomhedtaenradmacrocybecrassa AT suriyasassanarakit morphologicalcharacteristicsandmolecularidentificationofawildthaiisolateofthetropicalmushroomhedtaenradmacrocybecrassa AT acharapayapanon morphologicalcharacteristicsandmolecularidentificationofawildthaiisolateofthetropicalmushroomhedtaenradmacrocybecrassa AT suttipunkeawsompong morphologicalcharacteristicsandmolecularidentificationofawildthaiisolateofthetropicalmushroomhedtaenradmacrocybecrassa
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Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa)

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