Potential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin

Hyeryeon Oh,1,2 Jin Sil Lee,1,2 Daekyung Sung,1 Jong-Min Lim,3,4 Won Il Choi1 1Center for Convergence Bioceramic Materials, Convergence R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, Chungbuk 28160, Republic of Korea; 2School of Materials Science and Engineeri...

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Autores principales:	Oh H, Lee JS, Sung D, Lim JM, Choi WI
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	optimization lipid micelle reactive oxygen species ros scavenging cell proliferation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a1e6467272a942c593989423c84cc564
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spelling	oai:doaj.org-article:a1e6467272a942c593989423c84cc5642021-12-02T14:59:19ZPotential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin1178-2013https://doaj.org/article/a1e6467272a942c593989423c84cc5642020-11-01T00:00:00Zhttps://www.dovepress.com/potential-antioxidant-and-wound-healing-effect-of-nano-liposol-with-hi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Hyeryeon Oh,1,2 Jin Sil Lee,1,2 Daekyung Sung,1 Jong-Min Lim,3,4 Won Il Choi1 1Center for Convergence Bioceramic Materials, Convergence R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, Chungbuk 28160, Republic of Korea; 2School of Materials Science and Engineering and Department of Nanobio Materials and Electronics, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea; 3Department of Chemical Engineering, Soonchunhyang University, Asan, Chungnam 31538, Republic of Korea; 4Department of Electronic Materials and Devices Engineering, Soonchunhyang University, Asan, Chungnam 31538, Republic of KoreaCorrespondence: Jong-Min Lim Department of Electronic Materials and Devices EngineeringSoonchunhyang University, Asan, Chungnam 31538, Republic of KoreaTel +82 41 530 4961Fax +82 41 530 1717Email jmlim@sch.ac.krWon Il ChoiCenter for Convergence Bioceramic Materials, Convergence R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, Chungbuk 28160, Republic of KoreaTel +82 43 913 1513Fax +82 43 913 1597Email choi830509@kicet.re.krBackground: Astaxanthin (ASTA), a carotenoid, is a strong antioxidant. However, its application in functional foods, pharmaceuticals, and cosmetics remains limited due to its low aqueous solubility and stability. Several different encapsulating materials have been used to improve the stability and bioavailability of ASTA; however, the currently investigated nano-carriers for ASTA require additional improvements with regard to their loading capacity and stability.Methods: In this study, we developed lecithin nano-liposol (Lec NS) as a novel carrier of ASTA using a simple emulsion evaporation method. The physicochemical characteristics including hydrodynamic diameter, polydispersity index, surface charge and morphology were analyzed by DLS and TEM. The antioxidant activity of the ASTA-loaded Lec NS (ASTA@Lec NS) was evaluated using a DPPH radical scavenging assay and in vitro antioxidant assay. The study of in vitro wound healing efficacy was carried out to observe the beneficial effect of antioxidant activity of ASTA@Lec NS on cell migration.Results: ASTA@Lec NS showed improved stability and efficacy owing to improved aqueous solubility of ASTA inside Lec NS. Both in situ and in vitro antioxidant activities of ASTA@Lec NS were higher than that of bare ASTA and Lec NS. It also exhibited strong wound healing efficacy by regulation of ROS level in in vitro cell model.Conclusion: This study revealed that the encapsulation of ASTA into Lec NS using a wet phase transfer enhanced its physiological stability and bioavailability for effective scavenging of reactive oxygen species.Keywords: optimization, lipid micelle, reactive oxygen species, ROS scavenging, cell proliferationOh HLee JSSung DLim JMChoi WIDove Medical Pressarticleoptimizationlipid micellereactive oxygen speciesros scavengingcell proliferationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 9231-9240 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	optimization lipid micelle reactive oxygen species ros scavenging cell proliferation Medicine (General) R5-920
spellingShingle	optimization lipid micelle reactive oxygen species ros scavenging cell proliferation Medicine (General) R5-920 Oh H Lee JS Sung D Lim JM Choi WI Potential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin
description	Hyeryeon Oh,1,2 Jin Sil Lee,1,2 Daekyung Sung,1 Jong-Min Lim,3,4 Won Il Choi1 1Center for Convergence Bioceramic Materials, Convergence R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, Chungbuk 28160, Republic of Korea; 2School of Materials Science and Engineering and Department of Nanobio Materials and Electronics, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea; 3Department of Chemical Engineering, Soonchunhyang University, Asan, Chungnam 31538, Republic of Korea; 4Department of Electronic Materials and Devices Engineering, Soonchunhyang University, Asan, Chungnam 31538, Republic of KoreaCorrespondence: Jong-Min Lim Department of Electronic Materials and Devices EngineeringSoonchunhyang University, Asan, Chungnam 31538, Republic of KoreaTel +82 41 530 4961Fax +82 41 530 1717Email jmlim@sch.ac.krWon Il ChoiCenter for Convergence Bioceramic Materials, Convergence R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, Chungbuk 28160, Republic of KoreaTel +82 43 913 1513Fax +82 43 913 1597Email choi830509@kicet.re.krBackground: Astaxanthin (ASTA), a carotenoid, is a strong antioxidant. However, its application in functional foods, pharmaceuticals, and cosmetics remains limited due to its low aqueous solubility and stability. Several different encapsulating materials have been used to improve the stability and bioavailability of ASTA; however, the currently investigated nano-carriers for ASTA require additional improvements with regard to their loading capacity and stability.Methods: In this study, we developed lecithin nano-liposol (Lec NS) as a novel carrier of ASTA using a simple emulsion evaporation method. The physicochemical characteristics including hydrodynamic diameter, polydispersity index, surface charge and morphology were analyzed by DLS and TEM. The antioxidant activity of the ASTA-loaded Lec NS (ASTA@Lec NS) was evaluated using a DPPH radical scavenging assay and in vitro antioxidant assay. The study of in vitro wound healing efficacy was carried out to observe the beneficial effect of antioxidant activity of ASTA@Lec NS on cell migration.Results: ASTA@Lec NS showed improved stability and efficacy owing to improved aqueous solubility of ASTA inside Lec NS. Both in situ and in vitro antioxidant activities of ASTA@Lec NS were higher than that of bare ASTA and Lec NS. It also exhibited strong wound healing efficacy by regulation of ROS level in in vitro cell model.Conclusion: This study revealed that the encapsulation of ASTA into Lec NS using a wet phase transfer enhanced its physiological stability and bioavailability for effective scavenging of reactive oxygen species.Keywords: optimization, lipid micelle, reactive oxygen species, ROS scavenging, cell proliferation
format	article
author	Oh H Lee JS Sung D Lim JM Choi WI
author_facet	Oh H Lee JS Sung D Lim JM Choi WI
author_sort	Oh H
title	Potential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin
title_short	Potential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin
title_full	Potential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin
title_fullStr	Potential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin
title_full_unstemmed	Potential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin
title_sort	potential antioxidant and wound healing effect of nano-liposol with high loading amount of astaxanthin
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/a1e6467272a942c593989423c84cc564
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Potential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin

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