Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing.

Testosterone and alendronate have been identified as two bone healing compounds which, when combined, synergistically stimulate bone regeneration. This study describes the development of a novel ultrasonic spray coating for sustained release of ancillary amounts of testosterone and alendronate encap...

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Autores principales:	Cindy J J M van de Ven, Nicole E C Bakker, Dennis P Link, Edwin J W Geven, Jan A Gossen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1eec33547784d3b81f047958b97d1af
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spelling	oai:doaj.org-article:a1eec33547784d3b81f047958b97d1af2021-11-25T06:19:11ZSustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing.1932-620310.1371/journal.pone.0251864https://doaj.org/article/a1eec33547784d3b81f047958b97d1af2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251864https://doaj.org/toc/1932-6203Testosterone and alendronate have been identified as two bone healing compounds which, when combined, synergistically stimulate bone regeneration. This study describes the development of a novel ultrasonic spray coating for sustained release of ancillary amounts of testosterone and alendronate encapsulated in PLGA 5004A as a carrier. Due to the low amounts of testosterone and alendronate used, sensitive in vitro assays were developed to determine in vitro release. The ultrasonic spray coating technology was optimized for coating titanium screws and pericardial collagen membranes, with the aim to improve osseo-integration and (guided) bone regeneration, respectively, without interfering with their primary mode of action. In vitro release analysis of collagen membranes and screws showed up to 21 days sustained release of the compounds without a burst release. Subsequent preclinical studies in rat and rabbit models indicated that testosterone and alendronate coated membranes and screws significantly improved bone regeneration in vivo. Coated membranes significantly improved the formation of new bone in a critical size calvarial defect model in rats (by 160% compared to controls). Coated screws implanted in rabbit femoral condyles significantly improved bone implant contact (69% vs 54% in controls), bone mineral density (121%) and bone volume (119%) up to 1.3 mm from the implant. Based on the results obtained, we suggest that implants or membranes enabled with local sustained delivery of ancillary amounts of testosterone and alendronate can be a promising system to stimulate local bone regeneration resulting in improved osseo-integration of implants and improved healing of bone defects and fractures.Cindy J J M van de VenNicole E C BakkerDennis P LinkEdwin J W GevenJan A GossenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251864 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cindy J J M van de Ven Nicole E C Bakker Dennis P Link Edwin J W Geven Jan A Gossen Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing.
description	Testosterone and alendronate have been identified as two bone healing compounds which, when combined, synergistically stimulate bone regeneration. This study describes the development of a novel ultrasonic spray coating for sustained release of ancillary amounts of testosterone and alendronate encapsulated in PLGA 5004A as a carrier. Due to the low amounts of testosterone and alendronate used, sensitive in vitro assays were developed to determine in vitro release. The ultrasonic spray coating technology was optimized for coating titanium screws and pericardial collagen membranes, with the aim to improve osseo-integration and (guided) bone regeneration, respectively, without interfering with their primary mode of action. In vitro release analysis of collagen membranes and screws showed up to 21 days sustained release of the compounds without a burst release. Subsequent preclinical studies in rat and rabbit models indicated that testosterone and alendronate coated membranes and screws significantly improved bone regeneration in vivo. Coated membranes significantly improved the formation of new bone in a critical size calvarial defect model in rats (by 160% compared to controls). Coated screws implanted in rabbit femoral condyles significantly improved bone implant contact (69% vs 54% in controls), bone mineral density (121%) and bone volume (119%) up to 1.3 mm from the implant. Based on the results obtained, we suggest that implants or membranes enabled with local sustained delivery of ancillary amounts of testosterone and alendronate can be a promising system to stimulate local bone regeneration resulting in improved osseo-integration of implants and improved healing of bone defects and fractures.
format	article
author	Cindy J J M van de Ven Nicole E C Bakker Dennis P Link Edwin J W Geven Jan A Gossen
author_facet	Cindy J J M van de Ven Nicole E C Bakker Dennis P Link Edwin J W Geven Jan A Gossen
author_sort	Cindy J J M van de Ven
title	Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing.
title_short	Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing.
title_full	Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing.
title_fullStr	Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing.
title_full_unstemmed	Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing.
title_sort	sustained release of ancillary amounts of testosterone and alendronate from plga coated pericard membranes and implants to improve bone healing.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a1eec33547784d3b81f047958b97d1af
work_keys_str_mv	AT cindyjjmvandeven sustainedreleaseofancillaryamountsoftestosteroneandalendronatefromplgacoatedpericardmembranesandimplantstoimprovebonehealing AT nicoleecbakker sustainedreleaseofancillaryamountsoftestosteroneandalendronatefromplgacoatedpericardmembranesandimplantstoimprovebonehealing AT dennisplink sustainedreleaseofancillaryamountsoftestosteroneandalendronatefromplgacoatedpericardmembranesandimplantstoimprovebonehealing AT edwinjwgeven sustainedreleaseofancillaryamountsoftestosteroneandalendronatefromplgacoatedpericardmembranesandimplantstoimprovebonehealing AT janagossen sustainedreleaseofancillaryamountsoftestosteroneandalendronatefromplgacoatedpericardmembranesandimplantstoimprovebonehealing
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Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing.

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