A forward genetic approach in Chlamydomonas reinhardtii as a strategy for exploring starch catabolism.

A screen was recently developed to study the mobilization of starch in the unicellular green alga Chlamydomonas reinhardtii. This screen relies on starch synthesis accumulation during nitrogen starvation followed by the supply of nitrogen and the switch to darkness. Hence multiple regulatory network...

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Autores principales:	Hande Tunçay, Justin Findinier, Thierry Duchêne, Virginie Cogez, Charlotte Cousin, Gilles Peltier, Steven G Ball, David Dauvillée
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1f2295b538d4921bc311c412ef9bb8f
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spelling	oai:doaj.org-article:a1f2295b538d4921bc311c412ef9bb8f2021-11-18T08:57:06ZA forward genetic approach in Chlamydomonas reinhardtii as a strategy for exploring starch catabolism.1932-620310.1371/journal.pone.0074763https://doaj.org/article/a1f2295b538d4921bc311c412ef9bb8f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24019981/?tool=EBIhttps://doaj.org/toc/1932-6203A screen was recently developed to study the mobilization of starch in the unicellular green alga Chlamydomonas reinhardtii. This screen relies on starch synthesis accumulation during nitrogen starvation followed by the supply of nitrogen and the switch to darkness. Hence multiple regulatory networks including those of nutrient starvation, cell cycle control and light to dark transitions are likely to impact the recovery of mutant candidates. In this paper we monitor the specificity of this mutant screen by characterizing the nature of the genes disrupted in the selected mutants. We show that one third of the mutants consisted of strains mutated in genes previously reported to be of paramount importance in starch catabolism such as those encoding β-amylases, the maltose export protein, and branching enzyme I. The other mutants were defective for previously uncharacterized functions some of which are likely to define novel proteins affecting starch mobilization in green algae.Hande TunçayJustin FindinierThierry DuchêneVirginie CogezCharlotte CousinGilles PeltierSteven G BallDavid DauvilléePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74763 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hande Tunçay Justin Findinier Thierry Duchêne Virginie Cogez Charlotte Cousin Gilles Peltier Steven G Ball David Dauvillée A forward genetic approach in Chlamydomonas reinhardtii as a strategy for exploring starch catabolism.
description	A screen was recently developed to study the mobilization of starch in the unicellular green alga Chlamydomonas reinhardtii. This screen relies on starch synthesis accumulation during nitrogen starvation followed by the supply of nitrogen and the switch to darkness. Hence multiple regulatory networks including those of nutrient starvation, cell cycle control and light to dark transitions are likely to impact the recovery of mutant candidates. In this paper we monitor the specificity of this mutant screen by characterizing the nature of the genes disrupted in the selected mutants. We show that one third of the mutants consisted of strains mutated in genes previously reported to be of paramount importance in starch catabolism such as those encoding β-amylases, the maltose export protein, and branching enzyme I. The other mutants were defective for previously uncharacterized functions some of which are likely to define novel proteins affecting starch mobilization in green algae.
format	article
author	Hande Tunçay Justin Findinier Thierry Duchêne Virginie Cogez Charlotte Cousin Gilles Peltier Steven G Ball David Dauvillée
author_facet	Hande Tunçay Justin Findinier Thierry Duchêne Virginie Cogez Charlotte Cousin Gilles Peltier Steven G Ball David Dauvillée
author_sort	Hande Tunçay
title	A forward genetic approach in Chlamydomonas reinhardtii as a strategy for exploring starch catabolism.
title_short	A forward genetic approach in Chlamydomonas reinhardtii as a strategy for exploring starch catabolism.
title_full	A forward genetic approach in Chlamydomonas reinhardtii as a strategy for exploring starch catabolism.
title_fullStr	A forward genetic approach in Chlamydomonas reinhardtii as a strategy for exploring starch catabolism.
title_full_unstemmed	A forward genetic approach in Chlamydomonas reinhardtii as a strategy for exploring starch catabolism.
title_sort	forward genetic approach in chlamydomonas reinhardtii as a strategy for exploring starch catabolism.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a1f2295b538d4921bc311c412ef9bb8f
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A forward genetic approach in Chlamydomonas reinhardtii as a strategy for exploring starch catabolism.

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