Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.

Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able...

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Autores principales:	Lorena Setten, Gabriela Soto, Matteo Mozzicafreddo, Ana Romina Fox, Christian Lisi, Massimiliano Cuccioloni, Mauro Angeletti, Elba Pagano, Antonio Díaz-Paleo, Nicolás Daniel Ayub
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a1fb80eb370b4018a930b39363f66858
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spelling	oai:doaj.org-article:a1fb80eb370b4018a930b39363f668582021-11-18T07:45:58ZEngineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.1932-620310.1371/journal.pone.0063666https://doaj.org/article/a1fb80eb370b4018a930b39363f668582013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23675499/?tool=EBIhttps://doaj.org/toc/1932-6203Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able to grow in L medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. Pf-5 X940 also showed constitutive expression and enzymatic activity of nitrogenase in ammonium medium or in nitrogen-free medium, suggesting a constitutive nitrogen fixation. Similar to Pseudomonas protegens Pf-5, Pseudomonas putida, Pseudomonas veronii and Pseudomonas taetrolens but not Pseudomonas balearica and Pseudomonas stutzeri transformed with cosmid X940 showed constitutive nitrogenase activity and high ammonium production, suggesting that this phenotype depends on the genome context and that this technology to obtain nitrogen-fixing bacteria is not restricted to Pf-5. Interestingly, inoculation of Arabidopsis, alfalfa, tall fescue and maize with Pf-5 X940 increased the ammonium concentration in soil and plant productivity under nitrogen-deficient conditions. In conclusion, these results open the way to the production of effective recombinant inoculants for nitrogen fixation on a wide range of crops.Lorena SettenGabriela SotoMatteo MozzicafreddoAna Romina FoxChristian LisiMassimiliano CuccioloniMauro AngelettiElba PaganoAntonio Díaz-PaleoNicolás Daniel AyubPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63666 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lorena Setten Gabriela Soto Matteo Mozzicafreddo Ana Romina Fox Christian Lisi Massimiliano Cuccioloni Mauro Angeletti Elba Pagano Antonio Díaz-Paleo Nicolás Daniel Ayub Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.
description	Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able to grow in L medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. Pf-5 X940 also showed constitutive expression and enzymatic activity of nitrogenase in ammonium medium or in nitrogen-free medium, suggesting a constitutive nitrogen fixation. Similar to Pseudomonas protegens Pf-5, Pseudomonas putida, Pseudomonas veronii and Pseudomonas taetrolens but not Pseudomonas balearica and Pseudomonas stutzeri transformed with cosmid X940 showed constitutive nitrogenase activity and high ammonium production, suggesting that this phenotype depends on the genome context and that this technology to obtain nitrogen-fixing bacteria is not restricted to Pf-5. Interestingly, inoculation of Arabidopsis, alfalfa, tall fescue and maize with Pf-5 X940 increased the ammonium concentration in soil and plant productivity under nitrogen-deficient conditions. In conclusion, these results open the way to the production of effective recombinant inoculants for nitrogen fixation on a wide range of crops.
format	article
author	Lorena Setten Gabriela Soto Matteo Mozzicafreddo Ana Romina Fox Christian Lisi Massimiliano Cuccioloni Mauro Angeletti Elba Pagano Antonio Díaz-Paleo Nicolás Daniel Ayub
author_facet	Lorena Setten Gabriela Soto Matteo Mozzicafreddo Ana Romina Fox Christian Lisi Massimiliano Cuccioloni Mauro Angeletti Elba Pagano Antonio Díaz-Paleo Nicolás Daniel Ayub
author_sort	Lorena Setten
title	Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.
title_short	Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.
title_full	Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.
title_fullStr	Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.
title_full_unstemmed	Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.
title_sort	engineering pseudomonas protegens pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a1fb80eb370b4018a930b39363f66858
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Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.

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