LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis

Long non-coding RNAs are involved in many infectious diseases. Our previous studies showed that lncRNA-ENST00000421645 expression is increased in T lymphocytes of neurosyphilis patients compared to healthy controls. However, whether lncRNA-ENST00000421645 has biological functions remains unclear. Th...

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Autores principales:	Kai-Xuan Wu, Xiao-Tong Wang, Xin-Lin Hu, Xiao-Yong Jiang, Jing-Cong Zhuang, Yan-Zhu Xu, Li-Rong Lin, Man-Li Tong, Tian-Ci Yang, Li-Li Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	LncRNA-ENST00000421645 IFN-γ expression T cell apoptosis neurosyphilis Kank1 14-3-3 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a1ff6808d2164095a5c9c76e3e79cd06
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spelling	oai:doaj.org-article:a1ff6808d2164095a5c9c76e3e79cd062021-12-01T17:58:19ZLncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis1664-302X10.3389/fmicb.2021.749171https://doaj.org/article/a1ff6808d2164095a5c9c76e3e79cd062021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.749171/fullhttps://doaj.org/toc/1664-302XLong non-coding RNAs are involved in many infectious diseases. Our previous studies showed that lncRNA-ENST00000421645 expression is increased in T lymphocytes of neurosyphilis patients compared to healthy controls. However, whether lncRNA-ENST00000421645 has biological functions remains unclear. The current study was undertaken to understand the mechanism of lncRNA-ENST00000421645 in T lymphocyte function in neurosyphilis patients. The lncRNA-ENST00000421645 pull-down assay showed that lncRNA-ENST00000421645 acted on the acetylase NAT10. The chromatin immunoprecipitation (ChIP)-PCR results showed that lncRNA-ENST00000421645 promoted the acetylation of histone H3K27 adjacent to the Kank1 promoter, thereby promoting Kank1 protein expression. Kank1 promotes 14-3-3 protein expression, inhibits NF-kB activation, inhibits IFN-γ secretion by T lymphocytes, and promotes T lymphocyte apoptosis. Taken together, our findings suggest a novel mechanism that LncRNA-ENST00000421645 upregulates Kank1 to inhibit IFN-γ expression and promote T cell apoptosis in neurosyphilis.Kai-Xuan WuXiao-Tong WangXin-Lin HuXiao-Yong JiangJing-Cong ZhuangYan-Zhu XuLi-Rong LinMan-Li TongTian-Ci YangLi-Li LiuFrontiers Media S.A.articleLncRNA-ENST00000421645IFN-γ expressionT cell apoptosisneurosyphilisKank114-3-3MicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	LncRNA-ENST00000421645 IFN-γ expression T cell apoptosis neurosyphilis Kank1 14-3-3 Microbiology QR1-502
spellingShingle	LncRNA-ENST00000421645 IFN-γ expression T cell apoptosis neurosyphilis Kank1 14-3-3 Microbiology QR1-502 Kai-Xuan Wu Xiao-Tong Wang Xin-Lin Hu Xiao-Yong Jiang Jing-Cong Zhuang Yan-Zhu Xu Li-Rong Lin Man-Li Tong Tian-Ci Yang Li-Li Liu LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis
description	Long non-coding RNAs are involved in many infectious diseases. Our previous studies showed that lncRNA-ENST00000421645 expression is increased in T lymphocytes of neurosyphilis patients compared to healthy controls. However, whether lncRNA-ENST00000421645 has biological functions remains unclear. The current study was undertaken to understand the mechanism of lncRNA-ENST00000421645 in T lymphocyte function in neurosyphilis patients. The lncRNA-ENST00000421645 pull-down assay showed that lncRNA-ENST00000421645 acted on the acetylase NAT10. The chromatin immunoprecipitation (ChIP)-PCR results showed that lncRNA-ENST00000421645 promoted the acetylation of histone H3K27 adjacent to the Kank1 promoter, thereby promoting Kank1 protein expression. Kank1 promotes 14-3-3 protein expression, inhibits NF-kB activation, inhibits IFN-γ secretion by T lymphocytes, and promotes T lymphocyte apoptosis. Taken together, our findings suggest a novel mechanism that LncRNA-ENST00000421645 upregulates Kank1 to inhibit IFN-γ expression and promote T cell apoptosis in neurosyphilis.
format	article
author	Kai-Xuan Wu Xiao-Tong Wang Xin-Lin Hu Xiao-Yong Jiang Jing-Cong Zhuang Yan-Zhu Xu Li-Rong Lin Man-Li Tong Tian-Ci Yang Li-Li Liu
author_facet	Kai-Xuan Wu Xiao-Tong Wang Xin-Lin Hu Xiao-Yong Jiang Jing-Cong Zhuang Yan-Zhu Xu Li-Rong Lin Man-Li Tong Tian-Ci Yang Li-Li Liu
author_sort	Kai-Xuan Wu
title	LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis
title_short	LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis
title_full	LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis
title_fullStr	LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis
title_full_unstemmed	LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis
title_sort	lncrna-enst00000421645 upregulates kank1 to inhibit ifn-γ expression and promote t cell apoptosis in neurosyphilis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a1ff6808d2164095a5c9c76e3e79cd06
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LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis

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