Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway

L-theanine is a nonprotein amino acid found in tea leaves and has been widely used as a safe food additive in beverages or foods because of its varied bioactivities. The aim of this study was to reveal the in vitro gastrointestinal protective effects of L-theanine in DSS-induced intestinal porcine e...

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Autores principales:	Longlin Zhang, Mengmeng Ma, Zhengyi Li, Haihan Zhang, Xi He, Zehe Song
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	L-theanine proliferation IPEC-J2 cells metabolomics Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/a206b13dd4dc44aca46a7ba81bd54a69
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spelling	oai:doaj.org-article:a206b13dd4dc44aca46a7ba81bd54a692021-11-25T18:29:02ZProtective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway10.3390/molecules262270021420-3049https://doaj.org/article/a206b13dd4dc44aca46a7ba81bd54a692021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/7002https://doaj.org/toc/1420-3049L-theanine is a nonprotein amino acid found in tea leaves and has been widely used as a safe food additive in beverages or foods because of its varied bioactivities. The aim of this study was to reveal the in vitro gastrointestinal protective effects of L-theanine in DSS-induced intestinal porcine enterocyte (IPEC-J2) cell models using molecular and metabolic methods. Results showed that 2.5% dextran sulfate sodium (DSS) treatment inhibited the cell proliferation of IPEC-J2 and blocked the normal operation of the cell cycle, while L-theanine pretreatment significantly preserved these trends to exert protective effects. L-theanine pre-treatment also up-regulated the EGF, CDC2, FGF2, Rb genes and down-regulated p53, p21 proliferation-related mRNA expression in DSS-treated cells, in accompany with p53 signaling pathway inhibition. Meanwhile, metabolomics analysis revealed that L-theanine and DSS treated IPEC-J2 cells have different metabolomic profiles, with significant changes in the key metabolites involved in pyrimidine metabolism and amino acid metabolism, which play an important role in nucleotide metabolism. In summary, L-theanine has a beneficial protection in DSS-induced IPEC-J2 cells via promoting proliferation and regulating metabolism disorders.Longlin ZhangMengmeng MaZhengyi LiHaihan ZhangXi HeZehe SongMDPI AGarticleL-theanineproliferationIPEC-J2 cellsmetabolomicsOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 7002, p 7002 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	L-theanine proliferation IPEC-J2 cells metabolomics Organic chemistry QD241-441
spellingShingle	L-theanine proliferation IPEC-J2 cells metabolomics Organic chemistry QD241-441 Longlin Zhang Mengmeng Ma Zhengyi Li Haihan Zhang Xi He Zehe Song Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway
description	L-theanine is a nonprotein amino acid found in tea leaves and has been widely used as a safe food additive in beverages or foods because of its varied bioactivities. The aim of this study was to reveal the in vitro gastrointestinal protective effects of L-theanine in DSS-induced intestinal porcine enterocyte (IPEC-J2) cell models using molecular and metabolic methods. Results showed that 2.5% dextran sulfate sodium (DSS) treatment inhibited the cell proliferation of IPEC-J2 and blocked the normal operation of the cell cycle, while L-theanine pretreatment significantly preserved these trends to exert protective effects. L-theanine pre-treatment also up-regulated the EGF, CDC2, FGF2, Rb genes and down-regulated p53, p21 proliferation-related mRNA expression in DSS-treated cells, in accompany with p53 signaling pathway inhibition. Meanwhile, metabolomics analysis revealed that L-theanine and DSS treated IPEC-J2 cells have different metabolomic profiles, with significant changes in the key metabolites involved in pyrimidine metabolism and amino acid metabolism, which play an important role in nucleotide metabolism. In summary, L-theanine has a beneficial protection in DSS-induced IPEC-J2 cells via promoting proliferation and regulating metabolism disorders.
format	article
author	Longlin Zhang Mengmeng Ma Zhengyi Li Haihan Zhang Xi He Zehe Song
author_facet	Longlin Zhang Mengmeng Ma Zhengyi Li Haihan Zhang Xi He Zehe Song
author_sort	Longlin Zhang
title	Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway
title_short	Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway
title_full	Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway
title_fullStr	Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway
title_full_unstemmed	Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway
title_sort	protective effects of l-theanine on ipec-j2 cells growth inhibition induced by dextran sulfate sodium via p53 signaling pathway
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a206b13dd4dc44aca46a7ba81bd54a69
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Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway

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