Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF

The transcription factor Ets1 is essential for the development/differentiation of invariant Natural Killer T (iNKT) cells at multiple stages. However, its mechanisms of action and target genes in iNKT cells are still elusive. Here, we show that Ets1 is required for the optimal expression of the Vα14...

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Autores principales:	Ya-Ting Chuang, Wan-Chu Chuang, Chih-Chun Liu, Chia-Wei Liu, Yu-Wen Huang, Huang-Yu Yang, I-Cheng Ho, Tzong-Shyuan Tai
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	iNKT cells Ets1 ICOS PLZF Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/a20f2071ca934d14be469995d277e9ba
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spelling	oai:doaj.org-article:a20f2071ca934d14be469995d277e9ba2021-11-25T17:54:14ZEts1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF10.3390/ijms2222121991422-00671661-6596https://doaj.org/article/a20f2071ca934d14be469995d277e9ba2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12199https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The transcription factor Ets1 is essential for the development/differentiation of invariant Natural Killer T (iNKT) cells at multiple stages. However, its mechanisms of action and target genes in iNKT cells are still elusive. Here, we show that Ets1 is required for the optimal expression of the Vα14Jα18 T cell receptor (TCR) in post-selected thymic iNKT cells and their immediate differentiation. Ets1 is also critical for maintaining the peripheral homeostasis of iNKT cells, which is a role independent of the expression of the Vα14Jα18 TCR. Genome-wide transcriptomic analyses of post-selected iNKT cells further reveal that Ets1 controls leukocytes activation, proliferation differentiation, and leukocyte-mediated immunity. In addition, Ets1 regulates the expression of ICOS and PLZF in iNKT cells. More importantly, restoring the expression of PLZF and the Vα14Jα18 TCR partially rescues the differentiation of iNKT cells in the absence of Ets1. Taken together, our results establish a detailed molecular picture of how Ets1 regulates the stepwise differentiation of iNKT cells.Ya-Ting ChuangWan-Chu ChuangChih-Chun LiuChia-Wei LiuYu-Wen HuangHuang-Yu YangI-Cheng HoTzong-Shyuan TaiMDPI AGarticleiNKT cellsEts1ICOSPLZFBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12199, p 12199 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	iNKT cells Ets1 ICOS PLZF Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	iNKT cells Ets1 ICOS PLZF Biology (General) QH301-705.5 Chemistry QD1-999 Ya-Ting Chuang Wan-Chu Chuang Chih-Chun Liu Chia-Wei Liu Yu-Wen Huang Huang-Yu Yang I-Cheng Ho Tzong-Shyuan Tai Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF
description	The transcription factor Ets1 is essential for the development/differentiation of invariant Natural Killer T (iNKT) cells at multiple stages. However, its mechanisms of action and target genes in iNKT cells are still elusive. Here, we show that Ets1 is required for the optimal expression of the Vα14Jα18 T cell receptor (TCR) in post-selected thymic iNKT cells and their immediate differentiation. Ets1 is also critical for maintaining the peripheral homeostasis of iNKT cells, which is a role independent of the expression of the Vα14Jα18 TCR. Genome-wide transcriptomic analyses of post-selected iNKT cells further reveal that Ets1 controls leukocytes activation, proliferation differentiation, and leukocyte-mediated immunity. In addition, Ets1 regulates the expression of ICOS and PLZF in iNKT cells. More importantly, restoring the expression of PLZF and the Vα14Jα18 TCR partially rescues the differentiation of iNKT cells in the absence of Ets1. Taken together, our results establish a detailed molecular picture of how Ets1 regulates the stepwise differentiation of iNKT cells.
format	article
author	Ya-Ting Chuang Wan-Chu Chuang Chih-Chun Liu Chia-Wei Liu Yu-Wen Huang Huang-Yu Yang I-Cheng Ho Tzong-Shyuan Tai
author_facet	Ya-Ting Chuang Wan-Chu Chuang Chih-Chun Liu Chia-Wei Liu Yu-Wen Huang Huang-Yu Yang I-Cheng Ho Tzong-Shyuan Tai
author_sort	Ya-Ting Chuang
title	Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF
title_short	Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF
title_full	Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF
title_fullStr	Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF
title_full_unstemmed	Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF
title_sort	ets1 promotes the differentiation of post-selected inkt cells through regulation of the expression of vα14jα18 t cell receptor and plzf
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a20f2071ca934d14be469995d277e9ba
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Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF

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