Potential spread of mcr-9-carrying IncHI2 plasmids in Enterobacter hormaechei in Vietnam

ABSTRACT: Objectives: Mobile colistin resistance (mcr) genes are widely distributed around the world. To date, ten major variants of mcr genes are known (mcr-1 to mcr-10). However, only a few instances of Enterobacterales isolates harbouring mcr genes other than mcr-1 have been reported in Vietnam....

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Autores principales:	Van Thi Thu Ha, Linh Dieu Tran, Nguyen Thi Tuyet Mai, Aki Hirabayashi, Son Thai Nguyen, Hoang Huy Tran, Keigo Shibayama, Masato Suzuki
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Antimicrobial resistance Colistin Carbapenem Enterobacter cloacae complex Plasmid Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a2167ef64b1c4c3eb1da2009da4fef5d
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spelling	oai:doaj.org-article:a2167ef64b1c4c3eb1da2009da4fef5d2021-12-04T04:34:13ZPotential spread of mcr-9-carrying IncHI2 plasmids in Enterobacter hormaechei in Vietnam2213-716510.1016/j.jgar.2021.09.012https://doaj.org/article/a2167ef64b1c4c3eb1da2009da4fef5d2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2213716521002368https://doaj.org/toc/2213-7165ABSTRACT: Objectives: Mobile colistin resistance (mcr) genes are widely distributed around the world. To date, ten major variants of mcr genes are known (mcr-1 to mcr-10). However, only a few instances of Enterobacterales isolates harbouring mcr genes other than mcr-1 have been reported in Vietnam. The aim of this study was to investigate mcr-harbouring antimicrobial-resistant Enterobacterales isolates in Vietnam. Methods: Two mcr-9-harbouring Enterobacter hormaechei clinical isolates (NIHE14-1904 and MH17-539M) were obtained from medical institutions in Hanoi, Vietnam, in 2014 and 2017, respectively. Their genomes and plasmid sequences were analysed by short-read and long-read sequencing. Subsequently, comparative sequence analysis of their mcr-9-carrying plasmids was performed. Results: Strains NIHE14-1904 and MH17-539M belonged to sequence types ST916 and ST66, respectively, according to the Enterobacter cloacae multilocus sequence typing (MLST) scheme. NIHE14-1904 and MH17-539M harboured the mcr-9 gene on similar IncHI2 plasmids, namely pNIHE14-1904-mcr9 (373.1 kb) and pMH17-539M-mcr9 (289.3 kb), respectively. These plasmids were also highly identical to widespread IncHI2 plasmids that are often associated with mcr genes. Conclusion: For the first time, mcr-9-harbouring Enterobacterales isolates were detected in Vietnam, which carried mcr-9 on IncHI2 plasmids. The prevalence of such plasmids needs to be monitored in the future owing to their high dissemination.Van Thi Thu HaLinh Dieu TranNguyen Thi Tuyet MaiAki HirabayashiSon Thai NguyenHoang Huy TranKeigo ShibayamaMasato SuzukiElsevierarticleAntimicrobial resistanceColistinCarbapenemEnterobacter cloacae complexPlasmidMicrobiologyQR1-502ENJournal of Global Antimicrobial Resistance, Vol 27, Iss , Pp 332-334 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antimicrobial resistance Colistin Carbapenem Enterobacter cloacae complex Plasmid Microbiology QR1-502
spellingShingle	Antimicrobial resistance Colistin Carbapenem Enterobacter cloacae complex Plasmid Microbiology QR1-502 Van Thi Thu Ha Linh Dieu Tran Nguyen Thi Tuyet Mai Aki Hirabayashi Son Thai Nguyen Hoang Huy Tran Keigo Shibayama Masato Suzuki Potential spread of mcr-9-carrying IncHI2 plasmids in Enterobacter hormaechei in Vietnam
description	ABSTRACT: Objectives: Mobile colistin resistance (mcr) genes are widely distributed around the world. To date, ten major variants of mcr genes are known (mcr-1 to mcr-10). However, only a few instances of Enterobacterales isolates harbouring mcr genes other than mcr-1 have been reported in Vietnam. The aim of this study was to investigate mcr-harbouring antimicrobial-resistant Enterobacterales isolates in Vietnam. Methods: Two mcr-9-harbouring Enterobacter hormaechei clinical isolates (NIHE14-1904 and MH17-539M) were obtained from medical institutions in Hanoi, Vietnam, in 2014 and 2017, respectively. Their genomes and plasmid sequences were analysed by short-read and long-read sequencing. Subsequently, comparative sequence analysis of their mcr-9-carrying plasmids was performed. Results: Strains NIHE14-1904 and MH17-539M belonged to sequence types ST916 and ST66, respectively, according to the Enterobacter cloacae multilocus sequence typing (MLST) scheme. NIHE14-1904 and MH17-539M harboured the mcr-9 gene on similar IncHI2 plasmids, namely pNIHE14-1904-mcr9 (373.1 kb) and pMH17-539M-mcr9 (289.3 kb), respectively. These plasmids were also highly identical to widespread IncHI2 plasmids that are often associated with mcr genes. Conclusion: For the first time, mcr-9-harbouring Enterobacterales isolates were detected in Vietnam, which carried mcr-9 on IncHI2 plasmids. The prevalence of such plasmids needs to be monitored in the future owing to their high dissemination.
format	article
author	Van Thi Thu Ha Linh Dieu Tran Nguyen Thi Tuyet Mai Aki Hirabayashi Son Thai Nguyen Hoang Huy Tran Keigo Shibayama Masato Suzuki
author_facet	Van Thi Thu Ha Linh Dieu Tran Nguyen Thi Tuyet Mai Aki Hirabayashi Son Thai Nguyen Hoang Huy Tran Keigo Shibayama Masato Suzuki
author_sort	Van Thi Thu Ha
title	Potential spread of mcr-9-carrying IncHI2 plasmids in Enterobacter hormaechei in Vietnam
title_short	Potential spread of mcr-9-carrying IncHI2 plasmids in Enterobacter hormaechei in Vietnam
title_full	Potential spread of mcr-9-carrying IncHI2 plasmids in Enterobacter hormaechei in Vietnam
title_fullStr	Potential spread of mcr-9-carrying IncHI2 plasmids in Enterobacter hormaechei in Vietnam
title_full_unstemmed	Potential spread of mcr-9-carrying IncHI2 plasmids in Enterobacter hormaechei in Vietnam
title_sort	potential spread of mcr-9-carrying inchi2 plasmids in enterobacter hormaechei in vietnam
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/a2167ef64b1c4c3eb1da2009da4fef5d
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Potential spread of mcr-9-carrying IncHI2 plasmids in Enterobacter hormaechei in Vietnam

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