Blocking TRPV1 in nucleus accumbens inhibits persistent morphine conditioned place preference expression in rats.

The function of TRPV1 (transient receptor potential vanilloid subfamily, member 1) in the central nervous system is gradually elucidated. It has been recently proved to be expressed in nucleus accumbens (NAc), a region playing an essential role in mediating opioid craving and taking behaviors. Based...

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Autores principales: Li-Jun Heng, Bo Huang, Heng Guo, Lian-Ting Ma, Wei-Xin Yuan, Jian Song, Peng Wang, Guo-Zheng Xu, Guo-Dong Gao
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Publicado: Public Library of Science (PLoS) 2014
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spelling oai:doaj.org-article:a21a4817c79341b5834b61d6c74c434b2021-11-25T06:04:58ZBlocking TRPV1 in nucleus accumbens inhibits persistent morphine conditioned place preference expression in rats.1932-620310.1371/journal.pone.0104546https://doaj.org/article/a21a4817c79341b5834b61d6c74c434b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25118895/?tool=EBIhttps://doaj.org/toc/1932-6203The function of TRPV1 (transient receptor potential vanilloid subfamily, member 1) in the central nervous system is gradually elucidated. It has been recently proved to be expressed in nucleus accumbens (NAc), a region playing an essential role in mediating opioid craving and taking behaviors. Based on the general role of TRPV1 antagonist in blocking neural over-excitability by both pre- and post-synaptic mechanisms, TRPV1 antagonist capsazepine (CPZ) was tested for its ability to prohibit persistent opioid craving in rats. In the present study, we assessed the expression of TRPV1 in nucleus accumbens and investigated the effect of CPZ in bilateral nucleus accumbens on persistent morphine conditioned place preference (mCPP) in rats. We also evaluated the side-effect of CPZ on activity by comparing cross-beam times between groups. We found that morphine conditioned place preference increased the TRPV1 expression and CPZ attenuated morphine conditioned place preference in a dose-dependent and target-specific manner after both short- and long-term spontaneous withdrawal, reflected by the reduction of the increased time in morphine-paired side. CPZ (10 nM) could induce prolonged and stable inhibition of morphine conditioned place preference expression. More importantly, CPZ did not cause dysfunction of activity in the subjects tested, which indicates the inhibitory effect was not obtained at the sacrifice of regular movement. Collectively, these results indicated that injection of TRPV1 antagonist in nucleus accumbens is capable of attenuating persistent morphine conditioned place preference without affecting normal activity. Thus, TRPV1 antagonist is one of the promising therapeutic drugs for the treatment of opioid addiction.Li-Jun HengBo HuangHeng GuoLian-Ting MaWei-Xin YuanJian SongPeng WangGuo-Zheng XuGuo-Dong GaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104546 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Li-Jun Heng
Bo Huang
Heng Guo
Lian-Ting Ma
Wei-Xin Yuan
Jian Song
Peng Wang
Guo-Zheng Xu
Guo-Dong Gao
Blocking TRPV1 in nucleus accumbens inhibits persistent morphine conditioned place preference expression in rats.
description The function of TRPV1 (transient receptor potential vanilloid subfamily, member 1) in the central nervous system is gradually elucidated. It has been recently proved to be expressed in nucleus accumbens (NAc), a region playing an essential role in mediating opioid craving and taking behaviors. Based on the general role of TRPV1 antagonist in blocking neural over-excitability by both pre- and post-synaptic mechanisms, TRPV1 antagonist capsazepine (CPZ) was tested for its ability to prohibit persistent opioid craving in rats. In the present study, we assessed the expression of TRPV1 in nucleus accumbens and investigated the effect of CPZ in bilateral nucleus accumbens on persistent morphine conditioned place preference (mCPP) in rats. We also evaluated the side-effect of CPZ on activity by comparing cross-beam times between groups. We found that morphine conditioned place preference increased the TRPV1 expression and CPZ attenuated morphine conditioned place preference in a dose-dependent and target-specific manner after both short- and long-term spontaneous withdrawal, reflected by the reduction of the increased time in morphine-paired side. CPZ (10 nM) could induce prolonged and stable inhibition of morphine conditioned place preference expression. More importantly, CPZ did not cause dysfunction of activity in the subjects tested, which indicates the inhibitory effect was not obtained at the sacrifice of regular movement. Collectively, these results indicated that injection of TRPV1 antagonist in nucleus accumbens is capable of attenuating persistent morphine conditioned place preference without affecting normal activity. Thus, TRPV1 antagonist is one of the promising therapeutic drugs for the treatment of opioid addiction.
format article
author Li-Jun Heng
Bo Huang
Heng Guo
Lian-Ting Ma
Wei-Xin Yuan
Jian Song
Peng Wang
Guo-Zheng Xu
Guo-Dong Gao
author_facet Li-Jun Heng
Bo Huang
Heng Guo
Lian-Ting Ma
Wei-Xin Yuan
Jian Song
Peng Wang
Guo-Zheng Xu
Guo-Dong Gao
author_sort Li-Jun Heng
title Blocking TRPV1 in nucleus accumbens inhibits persistent morphine conditioned place preference expression in rats.
title_short Blocking TRPV1 in nucleus accumbens inhibits persistent morphine conditioned place preference expression in rats.
title_full Blocking TRPV1 in nucleus accumbens inhibits persistent morphine conditioned place preference expression in rats.
title_fullStr Blocking TRPV1 in nucleus accumbens inhibits persistent morphine conditioned place preference expression in rats.
title_full_unstemmed Blocking TRPV1 in nucleus accumbens inhibits persistent morphine conditioned place preference expression in rats.
title_sort blocking trpv1 in nucleus accumbens inhibits persistent morphine conditioned place preference expression in rats.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/a21a4817c79341b5834b61d6c74c434b
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