MUC5B leads to aggressive behavior of breast cancer MCF7 cells.

The mucin MUC5B has a critical protective function in the normal lung, salivary glands, esophagus, and gallbladder, and has been reported to be aberrantly expressed in breast cancer, the second leading cause of cancer-related deaths among women worldwide. To understand better the implication of MUC5...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hélène Valque, Valérie Gouyer, Frédéric Gottrand, Jean-Luc Desseyn
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2202772b1364d71bc36b221663de183
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a2202772b1364d71bc36b221663de183
record_format	dspace
spelling	oai:doaj.org-article:a2202772b1364d71bc36b221663de1832021-11-18T08:13:27ZMUC5B leads to aggressive behavior of breast cancer MCF7 cells.1932-620310.1371/journal.pone.0046699https://doaj.org/article/a2202772b1364d71bc36b221663de1832012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23056409/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The mucin MUC5B has a critical protective function in the normal lung, salivary glands, esophagus, and gallbladder, and has been reported to be aberrantly expressed in breast cancer, the second leading cause of cancer-related deaths among women worldwide. To understand better the implication of MUC5B in cancer pathogenesis, the luminal human breast cancer cell line MCF7 was transfected with a vector encoding a recombinant mini-mucin MUC5B and was then infected with a virus to deliver a short hairpin RNA to knock down the mini-mucin. The proliferative and invasive properties in Matrigel of MCF7 subclones and subpopulations were evaluated in vitro. A xenograft model was established by subcutaneous inoculation of MCF7 clones and subpopulations in SCID mice. Tumor growth was measured, and the tumors and metastases were assessed by histological and immunological analysis. The mini-mucin MUC5B promoted MCF7 cell proliferation and invasion in vitro. The xenograft experiments demonstrated that the mini-mucin promoted tumor growth and MCF7 cell dissemination. In conclusion, MUC5B expression is associated with aggressive behavior of MCF7 breast cancer cells. This study suggests that MUC5B may represent a good target for slowing tumor growth and metastasis.Hélène ValqueValérie GouyerFrédéric GottrandJean-Luc DesseynPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e46699 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hélène Valque Valérie Gouyer Frédéric Gottrand Jean-Luc Desseyn MUC5B leads to aggressive behavior of breast cancer MCF7 cells.
description	The mucin MUC5B has a critical protective function in the normal lung, salivary glands, esophagus, and gallbladder, and has been reported to be aberrantly expressed in breast cancer, the second leading cause of cancer-related deaths among women worldwide. To understand better the implication of MUC5B in cancer pathogenesis, the luminal human breast cancer cell line MCF7 was transfected with a vector encoding a recombinant mini-mucin MUC5B and was then infected with a virus to deliver a short hairpin RNA to knock down the mini-mucin. The proliferative and invasive properties in Matrigel of MCF7 subclones and subpopulations were evaluated in vitro. A xenograft model was established by subcutaneous inoculation of MCF7 clones and subpopulations in SCID mice. Tumor growth was measured, and the tumors and metastases were assessed by histological and immunological analysis. The mini-mucin MUC5B promoted MCF7 cell proliferation and invasion in vitro. The xenograft experiments demonstrated that the mini-mucin promoted tumor growth and MCF7 cell dissemination. In conclusion, MUC5B expression is associated with aggressive behavior of MCF7 breast cancer cells. This study suggests that MUC5B may represent a good target for slowing tumor growth and metastasis.
format	article
author	Hélène Valque Valérie Gouyer Frédéric Gottrand Jean-Luc Desseyn
author_facet	Hélène Valque Valérie Gouyer Frédéric Gottrand Jean-Luc Desseyn
author_sort	Hélène Valque
title	MUC5B leads to aggressive behavior of breast cancer MCF7 cells.
title_short	MUC5B leads to aggressive behavior of breast cancer MCF7 cells.
title_full	MUC5B leads to aggressive behavior of breast cancer MCF7 cells.
title_fullStr	MUC5B leads to aggressive behavior of breast cancer MCF7 cells.
title_full_unstemmed	MUC5B leads to aggressive behavior of breast cancer MCF7 cells.
title_sort	muc5b leads to aggressive behavior of breast cancer mcf7 cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a2202772b1364d71bc36b221663de183
work_keys_str_mv	AT helenevalque muc5bleadstoaggressivebehaviorofbreastcancermcf7cells AT valeriegouyer muc5bleadstoaggressivebehaviorofbreastcancermcf7cells AT fredericgottrand muc5bleadstoaggressivebehaviorofbreastcancermcf7cells AT jeanlucdesseyn muc5bleadstoaggressivebehaviorofbreastcancermcf7cells
_version_	1718422063245426688

MUC5B leads to aggressive behavior of breast cancer MCF7 cells.

Ejemplares similares