Corrigendum: Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wet-lands. MBMG 2: e21060. https://doi.org/10.3897/mbmg.2.21060

Chironomids have been proposed as important indicators for monitoring freshwater ecosystems, however, morphological determination is very challenging. In this study, we investigated the effectiveness of metabarcoding for chironomid diversity assessment and tested the retrieved chironomid operational...

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Autores principales:	Kathrin Theissinger, Anna Kästel, Vasco Elbrecht, Jenny Makkonen, Susanne Michiels, Susanne I. Schmidt, Stefanie Allgeier, Florian Leese, Carsten A. Brühl
Formato:	article
Lenguaje:	EN
Publicado:	Pensoft Publishers 2020
Materias:	Bacillus thuringensis var. israelensis (Bti) Barc Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/a235bafe9544467f805483f5a5296ffb
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spelling	oai:doaj.org-article:a235bafe9544467f805483f5a5296ffb2021-12-02T12:31:38ZCorrigendum: Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wet-lands. MBMG 2: e21060. https://doi.org/10.3897/mbmg.2.2106010.3897/mbmg.4.608542534-9708https://doaj.org/article/a235bafe9544467f805483f5a5296ffb2020-12-01T00:00:00Zhttps://mbmg.pensoft.net/article/60854/download/pdf/https://mbmg.pensoft.net/article/60854/download/xml/https://mbmg.pensoft.net/article/60854/https://doaj.org/toc/2534-9708Chironomids have been proposed as important indicators for monitoring freshwater ecosystems, however, morphological determination is very challenging. In this study, we investigated the effectiveness of metabarcoding for chironomid diversity assessment and tested the retrieved chironomid operational taxonomic units (OTUs) for possible changes in relative abundance and species diversity in relation to mosquito control actions in four temporary wetlands. The biocide Bacillus thuringiensis var. israelensis (Bti) is widely applied for mosquito control in temporary wetlands of the German Upper Rhine Valley. Even though Bti is considered environmentally friendly, several studies have shown non-target effects on chironomids, a key food resource in wetland ecosystems Three of the studied wetlands were, for the first year after 20 years of Bti treatment, partly left Bti-untreated in a split field design, and one wetland has never been treated with Bti. Our metabarcoding approach detected 54 chironomid OTUs across all study sites, of which 70% could be identified to species level comparisons against the BOLD database. We showed that metabarcoding increased chironomid species determination by 70%. However, we found only minor significant effects of Bti on the chironomid community composition. Subsequent studies will have to address if and how the chironomid community composition might change in the now Bti-untreated temporary wetlands to assess effects of Bti.Kathrin TheissingerAnna KästelVasco ElbrechtJenny MakkonenSusanne MichielsSusanne I. SchmidtStefanie AllgeierFlorian LeeseCarsten A. BrühlPensoft PublishersarticleBacillus thuringensis var. israelensis (Bti)BarcEcologyQH540-549.5ENMetabarcoding and Metagenomics, Vol 4, Iss , Pp 149-150 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacillus thuringensis var. israelensis (Bti) Barc Ecology QH540-549.5
spellingShingle	Bacillus thuringensis var. israelensis (Bti) Barc Ecology QH540-549.5 Kathrin Theissinger Anna Kästel Vasco Elbrecht Jenny Makkonen Susanne Michiels Susanne I. Schmidt Stefanie Allgeier Florian Leese Carsten A. Brühl Corrigendum: Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wet-lands. MBMG 2: e21060. https://doi.org/10.3897/mbmg.2.21060
description	Chironomids have been proposed as important indicators for monitoring freshwater ecosystems, however, morphological determination is very challenging. In this study, we investigated the effectiveness of metabarcoding for chironomid diversity assessment and tested the retrieved chironomid operational taxonomic units (OTUs) for possible changes in relative abundance and species diversity in relation to mosquito control actions in four temporary wetlands. The biocide Bacillus thuringiensis var. israelensis (Bti) is widely applied for mosquito control in temporary wetlands of the German Upper Rhine Valley. Even though Bti is considered environmentally friendly, several studies have shown non-target effects on chironomids, a key food resource in wetland ecosystems Three of the studied wetlands were, for the first year after 20 years of Bti treatment, partly left Bti-untreated in a split field design, and one wetland has never been treated with Bti. Our metabarcoding approach detected 54 chironomid OTUs across all study sites, of which 70% could be identified to species level comparisons against the BOLD database. We showed that metabarcoding increased chironomid species determination by 70%. However, we found only minor significant effects of Bti on the chironomid community composition. Subsequent studies will have to address if and how the chironomid community composition might change in the now Bti-untreated temporary wetlands to assess effects of Bti.
format	article
author	Kathrin Theissinger Anna Kästel Vasco Elbrecht Jenny Makkonen Susanne Michiels Susanne I. Schmidt Stefanie Allgeier Florian Leese Carsten A. Brühl
author_facet	Kathrin Theissinger Anna Kästel Vasco Elbrecht Jenny Makkonen Susanne Michiels Susanne I. Schmidt Stefanie Allgeier Florian Leese Carsten A. Brühl
author_sort	Kathrin Theissinger
title	Corrigendum: Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wet-lands. MBMG 2: e21060. https://doi.org/10.3897/mbmg.2.21060
title_short	Corrigendum: Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wet-lands. MBMG 2: e21060. https://doi.org/10.3897/mbmg.2.21060
title_full	Corrigendum: Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wet-lands. MBMG 2: e21060. https://doi.org/10.3897/mbmg.2.21060
title_fullStr	Corrigendum: Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wet-lands. MBMG 2: e21060. https://doi.org/10.3897/mbmg.2.21060
title_full_unstemmed	Corrigendum: Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wet-lands. MBMG 2: e21060. https://doi.org/10.3897/mbmg.2.21060
title_sort	corrigendum: using dna metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wet-lands. mbmg 2: e21060. https://doi.org/10.3897/mbmg.2.21060
publisher	Pensoft Publishers
publishDate	2020
url	https://doaj.org/article/a235bafe9544467f805483f5a5296ffb
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Corrigendum: Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wet-lands. MBMG 2: e21060. https://doi.org/10.3897/mbmg.2.21060

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