DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.

Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA fragmentation factor 40 kDa (DFF40), is one of the maj...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ryushin Mizuta, Shinsuke Araki, Makoto Furukawa, Yuki Furukawa, Syota Ebara, Daisuke Shiokawa, Katsuhiko Hayashi, Sei-ichi Tanuma, Daisuke Kitamura
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a23727df6fcc41cfba0a04177ec0ccc6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a23727df6fcc41cfba0a04177ec0ccc6
record_format	dspace
spelling	oai:doaj.org-article:a23727df6fcc41cfba0a04177ec0ccc62021-11-18T08:44:02ZDNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.1932-620310.1371/journal.pone.0080223https://doaj.org/article/a23727df6fcc41cfba0a04177ec0ccc62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24312463/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA fragmentation factor 40 kDa (DFF40), is one of the major effector endonucleases. DNase γ, a Mg(2+)/Ca(2+)-dependent endonuclease, is also known to generate INDF but its role among other apoptosis-associated endonucleases in cell death is unclear. Here we show that (i) INDF occurs even during necrosis in cell lines, primary cells, and in tissues of mice in vivo, and (ii) DNase γ, but not CAD, is the effector endonuclease for INDF in cells undergoing necrosis. These results document a previously unappreciated role for INDF in necrosis and define its molecular basis.Ryushin MizutaShinsuke ArakiMakoto FurukawaYuki FurukawaSyota EbaraDaisuke ShiokawaKatsuhiko HayashiSei-ichi TanumaDaisuke KitamuraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e80223 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryushin Mizuta Shinsuke Araki Makoto Furukawa Yuki Furukawa Syota Ebara Daisuke Shiokawa Katsuhiko Hayashi Sei-ichi Tanuma Daisuke Kitamura DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.
description	Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA fragmentation factor 40 kDa (DFF40), is one of the major effector endonucleases. DNase γ, a Mg(2+)/Ca(2+)-dependent endonuclease, is also known to generate INDF but its role among other apoptosis-associated endonucleases in cell death is unclear. Here we show that (i) INDF occurs even during necrosis in cell lines, primary cells, and in tissues of mice in vivo, and (ii) DNase γ, but not CAD, is the effector endonuclease for INDF in cells undergoing necrosis. These results document a previously unappreciated role for INDF in necrosis and define its molecular basis.
format	article
author	Ryushin Mizuta Shinsuke Araki Makoto Furukawa Yuki Furukawa Syota Ebara Daisuke Shiokawa Katsuhiko Hayashi Sei-ichi Tanuma Daisuke Kitamura
author_facet	Ryushin Mizuta Shinsuke Araki Makoto Furukawa Yuki Furukawa Syota Ebara Daisuke Shiokawa Katsuhiko Hayashi Sei-ichi Tanuma Daisuke Kitamura
author_sort	Ryushin Mizuta
title	DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.
title_short	DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.
title_full	DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.
title_fullStr	DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.
title_full_unstemmed	DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.
title_sort	dnase γ is the effector endonuclease for internucleosomal dna fragmentation in necrosis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a23727df6fcc41cfba0a04177ec0ccc6
work_keys_str_mv	AT ryushinmizuta dnasegistheeffectorendonucleaseforinternucleosomaldnafragmentationinnecrosis AT shinsukearaki dnasegistheeffectorendonucleaseforinternucleosomaldnafragmentationinnecrosis AT makotofurukawa dnasegistheeffectorendonucleaseforinternucleosomaldnafragmentationinnecrosis AT yukifurukawa dnasegistheeffectorendonucleaseforinternucleosomaldnafragmentationinnecrosis AT syotaebara dnasegistheeffectorendonucleaseforinternucleosomaldnafragmentationinnecrosis AT daisukeshiokawa dnasegistheeffectorendonucleaseforinternucleosomaldnafragmentationinnecrosis AT katsuhikohayashi dnasegistheeffectorendonucleaseforinternucleosomaldnafragmentationinnecrosis AT seiichitanuma dnasegistheeffectorendonucleaseforinternucleosomaldnafragmentationinnecrosis AT daisukekitamura dnasegistheeffectorendonucleaseforinternucleosomaldnafragmentationinnecrosis
_version_	1718421400453120000

DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.

Ejemplares similares