Genotype determination of megalocytivirus from Indonesian Marine Fishes

Murwantoko, Sari DWK, Handayani CR, Whittington RJ. 2018. Genotype determination of megalocytivirus from Indonesian Marine Fishes. Biodiversitas 19: 1730-1736. Megalocytivirus is the newest genus within the family of Iridoviridae which can be divided into groups represented by red sea bream iridovir...

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Autores principales:	MURWANTOKO MURWANTOKO, DINI WAHYU KARTIKA SARI, CHRISTINA RETNO HANDAYANI, RICHARD J. WHITTINGTON
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	genotype isknv marine fish megalocytivirus rsiv Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a240930f71c24c4eabc30a7b99d414d2
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spelling	oai:doaj.org-article:a240930f71c24c4eabc30a7b99d414d22021-11-16T14:04:05ZGenotype determination of megalocytivirus from Indonesian Marine Fishes1412-033X2085-472210.13057/biodiv/d190519https://doaj.org/article/a240930f71c24c4eabc30a7b99d414d22018-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2936https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Murwantoko, Sari DWK, Handayani CR, Whittington RJ. 2018. Genotype determination of megalocytivirus from Indonesian Marine Fishes. Biodiversitas 19: 1730-1736. Megalocytivirus is the newest genus within the family of Iridoviridae which can be divided into groups represented by red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV) and turbot reddish body iridovirus (TRBIV), threespine stickleback iridovirus (TSIV). This virus caused serious systemic disease in cultured marine fishes for consumption and ornamental freshwater fishes with significant mortality. The objective of this study was to determine the genotype of megalocytivirus which infected marine fishes from Lampung, Karimun Jawa, Situbondo and Batam based on major capsid protein (MCP), ATPase, DNA polymerase, CY15 and IRB6. The liver, spleen and kidney tissues of humpback grouper (Cromileptes altivelis), tiger grouper (Epinephelus fuscoguttatus), baramundi (Lates calcarifer) were fixed in 10% phosphate-buffered formalin for histological and fixed in 70% ethanol for molecular analysis. Molecular analysis was performed by amplification of MCP, ATPase, DNA polymerase, CY15 and IRB6 genes and followed by sequencing. Genotype was determined by alignment of the sequences with various genotypes of megalocytivirus from Genbank. Histological examination showed that hypertrophy, inclusion body forming bearing cells were found in liver, spleen and kidney tissues. Polymerase chain reaction with MCP primer produced specific DNA bands. Those results confirmed the infection of megalocytivirus on marine cultured fish samples. The analysis from 10 isolates on five genes revealed that two genotypes of megalocytivirus as infectious spleen and kidney necrosis virus (ISKNV) and red sea bream iridovirus (RSIV) genotypes were existed in Indonesia. The ISKNV genotype was confirmed in fish samples from Lampung, Jepara, Bali; while RSIV genotype was found in fishes from Batam, and Situbondo. Interestingly, both ISKNV and RSIV genotypes were confirmed in fish samples from Karimun Jawa. This paper is the first report on the present of ISKNV and RSIV genotypes in Indonesia based on MCP, ATPase, DNA polymerase, CY15 and IRB6 genes.MURWANTOKO MURWANTOKODINI WAHYU KARTIKA SARICHRISTINA RETNO HANDAYANIRICHARD J. WHITTINGTONMBI & UNS Soloarticlegenotypeisknvmarine fishmegalocytivirusrsivBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 5, Pp 1730-1736 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	genotype isknv marine fish megalocytivirus rsiv Biology (General) QH301-705.5
spellingShingle	genotype isknv marine fish megalocytivirus rsiv Biology (General) QH301-705.5 MURWANTOKO MURWANTOKO DINI WAHYU KARTIKA SARI CHRISTINA RETNO HANDAYANI RICHARD J. WHITTINGTON Genotype determination of megalocytivirus from Indonesian Marine Fishes
description	Murwantoko, Sari DWK, Handayani CR, Whittington RJ. 2018. Genotype determination of megalocytivirus from Indonesian Marine Fishes. Biodiversitas 19: 1730-1736. Megalocytivirus is the newest genus within the family of Iridoviridae which can be divided into groups represented by red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV) and turbot reddish body iridovirus (TRBIV), threespine stickleback iridovirus (TSIV). This virus caused serious systemic disease in cultured marine fishes for consumption and ornamental freshwater fishes with significant mortality. The objective of this study was to determine the genotype of megalocytivirus which infected marine fishes from Lampung, Karimun Jawa, Situbondo and Batam based on major capsid protein (MCP), ATPase, DNA polymerase, CY15 and IRB6. The liver, spleen and kidney tissues of humpback grouper (Cromileptes altivelis), tiger grouper (Epinephelus fuscoguttatus), baramundi (Lates calcarifer) were fixed in 10% phosphate-buffered formalin for histological and fixed in 70% ethanol for molecular analysis. Molecular analysis was performed by amplification of MCP, ATPase, DNA polymerase, CY15 and IRB6 genes and followed by sequencing. Genotype was determined by alignment of the sequences with various genotypes of megalocytivirus from Genbank. Histological examination showed that hypertrophy, inclusion body forming bearing cells were found in liver, spleen and kidney tissues. Polymerase chain reaction with MCP primer produced specific DNA bands. Those results confirmed the infection of megalocytivirus on marine cultured fish samples. The analysis from 10 isolates on five genes revealed that two genotypes of megalocytivirus as infectious spleen and kidney necrosis virus (ISKNV) and red sea bream iridovirus (RSIV) genotypes were existed in Indonesia. The ISKNV genotype was confirmed in fish samples from Lampung, Jepara, Bali; while RSIV genotype was found in fishes from Batam, and Situbondo. Interestingly, both ISKNV and RSIV genotypes were confirmed in fish samples from Karimun Jawa. This paper is the first report on the present of ISKNV and RSIV genotypes in Indonesia based on MCP, ATPase, DNA polymerase, CY15 and IRB6 genes.
format	article
author	MURWANTOKO MURWANTOKO DINI WAHYU KARTIKA SARI CHRISTINA RETNO HANDAYANI RICHARD J. WHITTINGTON
author_facet	MURWANTOKO MURWANTOKO DINI WAHYU KARTIKA SARI CHRISTINA RETNO HANDAYANI RICHARD J. WHITTINGTON
author_sort	MURWANTOKO MURWANTOKO
title	Genotype determination of megalocytivirus from Indonesian Marine Fishes
title_short	Genotype determination of megalocytivirus from Indonesian Marine Fishes
title_full	Genotype determination of megalocytivirus from Indonesian Marine Fishes
title_fullStr	Genotype determination of megalocytivirus from Indonesian Marine Fishes
title_full_unstemmed	Genotype determination of megalocytivirus from Indonesian Marine Fishes
title_sort	genotype determination of megalocytivirus from indonesian marine fishes
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/a240930f71c24c4eabc30a7b99d414d2
work_keys_str_mv	AT murwantokomurwantoko genotypedeterminationofmegalocytivirusfromindonesianmarinefishes AT diniwahyukartikasari genotypedeterminationofmegalocytivirusfromindonesianmarinefishes AT christinaretnohandayani genotypedeterminationofmegalocytivirusfromindonesianmarinefishes AT richardjwhittington genotypedeterminationofmegalocytivirusfromindonesianmarinefishes
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Genotype determination of megalocytivirus from Indonesian Marine Fishes

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