Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a malignant B cell lymphoma. However, the mechanisms of BLV-associated lymphomagenesis remain poorly understood. Here, after deep sequencing, we performed comparative analyses of B cell microRNAs (miRNAs) in cattle...

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Autores principales:	Chihiro Ochiai, Sonoko Miyauchi, Yuta Kudo, Yuta Naruke, Syuji Yoneyama, Keisuke Tomita, Leng Dongze, Yusuke Chiba, To-Ichi Hirata, Toshihiro Ichijo, Kazuya Nagai, Sota Kobayashi, Shinji Yamada, Hirokazu Hikono, Kenji Murakami
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a240ec0b8aea426e801200ef46ebc662
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spelling	oai:doaj.org-article:a240ec0b8aea426e801200ef46ebc6622021-12-02T20:14:42ZCharacterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.1932-620310.1371/journal.pone.0256588https://doaj.org/article/a240ec0b8aea426e801200ef46ebc6622021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256588https://doaj.org/toc/1932-6203Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a malignant B cell lymphoma. However, the mechanisms of BLV-associated lymphomagenesis remain poorly understood. Here, after deep sequencing, we performed comparative analyses of B cell microRNAs (miRNAs) in cattle infected with BLV and those without BLV. In BLV-infected cattle, BLV-derived miRNAs (blv-miRNAs) accounted for 38% of all miRNAs in B cells. Four of these blv-miRNAs (blv-miR-B1-5p, blv-miR-B2-5p, blv-miR-B4-3p, and blv-miR-B5-5p) had highly significant positive correlations with BLV proviral load (PVL). The read counts of 90 host-derived miRNAs (bta-miRNAs) were significantly down-regulated in BLV-infected cattle compared to those in uninfected cattle. Only bta-miR-375 had a positive correlation with PVL in BLV-infected cattle and was highly expressed in the B cell lymphoma tissue of EBL cattle. There were a few bta-miRNAs that correlated with BLV tax/rex gene expression; however, BLV AS1 expression had a significant negative correlation with many of the down-regulated bta-miRNAs that are important for tumor development and/or tumor suppression. These results suggest that BLV promotes lymphomagenesis via AS1 and blv-miRNAs, rather than tax/rex, by down-regulating the expression of bta-miRNAs that have a tumor-suppressing function, and this downregulation is linked to increased PVL.Chihiro OchiaiSonoko MiyauchiYuta KudoYuta NarukeSyuji YoneyamaKeisuke TomitaLeng DongzeYusuke ChibaTo-Ichi HirataToshihiro IchijoKazuya NagaiSota KobayashiShinji YamadaHirokazu HikonoKenji MurakamiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256588 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chihiro Ochiai Sonoko Miyauchi Yuta Kudo Yuta Naruke Syuji Yoneyama Keisuke Tomita Leng Dongze Yusuke Chiba To-Ichi Hirata Toshihiro Ichijo Kazuya Nagai Sota Kobayashi Shinji Yamada Hirokazu Hikono Kenji Murakami Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.
description	Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a malignant B cell lymphoma. However, the mechanisms of BLV-associated lymphomagenesis remain poorly understood. Here, after deep sequencing, we performed comparative analyses of B cell microRNAs (miRNAs) in cattle infected with BLV and those without BLV. In BLV-infected cattle, BLV-derived miRNAs (blv-miRNAs) accounted for 38% of all miRNAs in B cells. Four of these blv-miRNAs (blv-miR-B1-5p, blv-miR-B2-5p, blv-miR-B4-3p, and blv-miR-B5-5p) had highly significant positive correlations with BLV proviral load (PVL). The read counts of 90 host-derived miRNAs (bta-miRNAs) were significantly down-regulated in BLV-infected cattle compared to those in uninfected cattle. Only bta-miR-375 had a positive correlation with PVL in BLV-infected cattle and was highly expressed in the B cell lymphoma tissue of EBL cattle. There were a few bta-miRNAs that correlated with BLV tax/rex gene expression; however, BLV AS1 expression had a significant negative correlation with many of the down-regulated bta-miRNAs that are important for tumor development and/or tumor suppression. These results suggest that BLV promotes lymphomagenesis via AS1 and blv-miRNAs, rather than tax/rex, by down-regulating the expression of bta-miRNAs that have a tumor-suppressing function, and this downregulation is linked to increased PVL.
format	article
author	Chihiro Ochiai Sonoko Miyauchi Yuta Kudo Yuta Naruke Syuji Yoneyama Keisuke Tomita Leng Dongze Yusuke Chiba To-Ichi Hirata Toshihiro Ichijo Kazuya Nagai Sota Kobayashi Shinji Yamada Hirokazu Hikono Kenji Murakami
author_facet	Chihiro Ochiai Sonoko Miyauchi Yuta Kudo Yuta Naruke Syuji Yoneyama Keisuke Tomita Leng Dongze Yusuke Chiba To-Ichi Hirata Toshihiro Ichijo Kazuya Nagai Sota Kobayashi Shinji Yamada Hirokazu Hikono Kenji Murakami
author_sort	Chihiro Ochiai
title	Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.
title_short	Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.
title_full	Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.
title_fullStr	Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.
title_full_unstemmed	Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.
title_sort	characterization of microrna expression in b cells derived from japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a240ec0b8aea426e801200ef46ebc662
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Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.

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