Herbo-mineral formulation ‘Ashwashila’ attenuates rheumatoid arthritis symptoms in collagen-antibody-induced arthritis (CAIA) mice model

Abstract Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disorder that affects joints of hands and feet and introduces injury in secondary organs such as cardiac tissue. In the present study, we induced RA in male Balb/c mice (CAIA) using collagen-antibody cocktail (C-Ab) and lipopoly...

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Autores principales:	Acharya Balkrishna, Sachin Shridhar Sakat, Kheemraj Joshi, Sandeep Paudel, Deepika Joshi, Kamal Joshi, Ravikant Ranjan, Abhishek Gupta, Kunal Bhattacharya, Anurag Varshney
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Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a244eb2c48b841ceb1da2615a14fcc94
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spelling	oai:doaj.org-article:a244eb2c48b841ceb1da2615a14fcc942021-12-02T15:10:04ZHerbo-mineral formulation ‘Ashwashila’ attenuates rheumatoid arthritis symptoms in collagen-antibody-induced arthritis (CAIA) mice model10.1038/s41598-019-44485-92045-2322https://doaj.org/article/a244eb2c48b841ceb1da2615a14fcc942019-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-44485-9https://doaj.org/toc/2045-2322Abstract Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disorder that affects joints of hands and feet and introduces injury in secondary organs such as cardiac tissue. In the present study, we induced RA in male Balb/c mice (CAIA) using collagen-antibody cocktail (C-Ab) and lipopolysaccharide intraperitoneal injections. Induction of RA in the animals was detected through the loss of body weight, food, and water consumption, pedal edema, increased arthritis score of the paw and ankle, increase in radiological and histological lesion score of ankle and knee joints and enhanced pain perception in the C-Ab induced RA animals. Ashwashila is a herbo-mineral medicine from Indian Ayurvedic system. Human equivalent doses of Ashwashila (ASHW) and standard of care, Methotrexate were given to the CAIA animals for two weeks. ASHW treatment significantly reversed the effect of C-Ab with reduced pedal edema, arthritis score, radiological and histological lesion scores in ankle-joint, knee-joint and articular cartilage, reduced pain perception. These effects were comparable with the Methotrexate treatment. In human monocytic (THP-1) cells, ASHW was found to be biocompatible at in-vitro test doses. The anti-arthritis mechanism of action for ASHW was established through the suppression of pro-inflammatory cytokines such as IL-1β, IL-6, TNF-α; and upstream regulator, NF-κB. Taken together, we show the pre-clinical efficacy of ASHW in reducing RA associated symptoms by controlling inflammation and suggest it as a potential therapeutic candidate for rheumatoid arthritis.Acharya BalkrishnaSachin Shridhar SakatKheemraj JoshiSandeep PaudelDeepika JoshiKamal JoshiRavikant RanjanAbhishek GuptaKunal BhattacharyaAnurag VarshneyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-17 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Acharya Balkrishna Sachin Shridhar Sakat Kheemraj Joshi Sandeep Paudel Deepika Joshi Kamal Joshi Ravikant Ranjan Abhishek Gupta Kunal Bhattacharya Anurag Varshney Herbo-mineral formulation ‘Ashwashila’ attenuates rheumatoid arthritis symptoms in collagen-antibody-induced arthritis (CAIA) mice model
description	Abstract Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disorder that affects joints of hands and feet and introduces injury in secondary organs such as cardiac tissue. In the present study, we induced RA in male Balb/c mice (CAIA) using collagen-antibody cocktail (C-Ab) and lipopolysaccharide intraperitoneal injections. Induction of RA in the animals was detected through the loss of body weight, food, and water consumption, pedal edema, increased arthritis score of the paw and ankle, increase in radiological and histological lesion score of ankle and knee joints and enhanced pain perception in the C-Ab induced RA animals. Ashwashila is a herbo-mineral medicine from Indian Ayurvedic system. Human equivalent doses of Ashwashila (ASHW) and standard of care, Methotrexate were given to the CAIA animals for two weeks. ASHW treatment significantly reversed the effect of C-Ab with reduced pedal edema, arthritis score, radiological and histological lesion scores in ankle-joint, knee-joint and articular cartilage, reduced pain perception. These effects were comparable with the Methotrexate treatment. In human monocytic (THP-1) cells, ASHW was found to be biocompatible at in-vitro test doses. The anti-arthritis mechanism of action for ASHW was established through the suppression of pro-inflammatory cytokines such as IL-1β, IL-6, TNF-α; and upstream regulator, NF-κB. Taken together, we show the pre-clinical efficacy of ASHW in reducing RA associated symptoms by controlling inflammation and suggest it as a potential therapeutic candidate for rheumatoid arthritis.
format	article
author	Acharya Balkrishna Sachin Shridhar Sakat Kheemraj Joshi Sandeep Paudel Deepika Joshi Kamal Joshi Ravikant Ranjan Abhishek Gupta Kunal Bhattacharya Anurag Varshney
author_facet	Acharya Balkrishna Sachin Shridhar Sakat Kheemraj Joshi Sandeep Paudel Deepika Joshi Kamal Joshi Ravikant Ranjan Abhishek Gupta Kunal Bhattacharya Anurag Varshney
author_sort	Acharya Balkrishna
title	Herbo-mineral formulation ‘Ashwashila’ attenuates rheumatoid arthritis symptoms in collagen-antibody-induced arthritis (CAIA) mice model
title_short	Herbo-mineral formulation ‘Ashwashila’ attenuates rheumatoid arthritis symptoms in collagen-antibody-induced arthritis (CAIA) mice model
title_full	Herbo-mineral formulation ‘Ashwashila’ attenuates rheumatoid arthritis symptoms in collagen-antibody-induced arthritis (CAIA) mice model
title_fullStr	Herbo-mineral formulation ‘Ashwashila’ attenuates rheumatoid arthritis symptoms in collagen-antibody-induced arthritis (CAIA) mice model
title_full_unstemmed	Herbo-mineral formulation ‘Ashwashila’ attenuates rheumatoid arthritis symptoms in collagen-antibody-induced arthritis (CAIA) mice model
title_sort	herbo-mineral formulation ‘ashwashila’ attenuates rheumatoid arthritis symptoms in collagen-antibody-induced arthritis (caia) mice model
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/a244eb2c48b841ceb1da2615a14fcc94
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Herbo-mineral formulation ‘Ashwashila’ attenuates rheumatoid arthritis symptoms in collagen-antibody-induced arthritis (CAIA) mice model

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