Whole blood gene expression within days after total-body irradiation predicts long term survival in Gottingen minipigs

Abstract Gottingen minipigs mirror the physiological radiation response observed in humans and hence make an ideal candidate model for studying radiation biodosimetry for both limited-sized and mass casualty incidents. We examined the whole blood gene expression profiles starting one day after total...

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Autores principales:	Sunita Chopra, Maria Moroni, Jaleal Sanjak, Laurel MacMillan, Bernadette Hritzo, Shannon Martello, Michelle Bylicky, Jared May, C. Norman Coleman, Molykutty J. Aryankalayil
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2516ae3c6894857babcede544e3384f
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spelling	oai:doaj.org-article:a2516ae3c6894857babcede544e3384f2021-12-02T17:06:31ZWhole blood gene expression within days after total-body irradiation predicts long term survival in Gottingen minipigs10.1038/s41598-021-95120-52045-2322https://doaj.org/article/a2516ae3c6894857babcede544e3384f2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95120-5https://doaj.org/toc/2045-2322Abstract Gottingen minipigs mirror the physiological radiation response observed in humans and hence make an ideal candidate model for studying radiation biodosimetry for both limited-sized and mass casualty incidents. We examined the whole blood gene expression profiles starting one day after total-body irradiation with increasing doses of gamma-rays. The minipigs were monitored for up to 45 days or time to euthanasia necessitated by radiation effects. We successfully identified dose- and time-agnostic (over a 1–7 day period after radiation), survival-predictive gene expression signatures derived using machine-learning algorithms with high sensitivity and specificity. These survival-predictive signatures fare better than an optimally performing dose-differentiating signature or blood cellular profiles. These findings suggest that prediction of survival is a much more useful parameter for making triage, resource-utilization and treatment decisions in a resource-constrained environment compared to predictions of total dose received. It should hopefully be possible to build such classifiers for humans in the future.Sunita ChopraMaria MoroniJaleal SanjakLaurel MacMillanBernadette HritzoShannon MartelloMichelle BylickyJared MayC. Norman ColemanMolykutty J. AryankalayilNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sunita Chopra Maria Moroni Jaleal Sanjak Laurel MacMillan Bernadette Hritzo Shannon Martello Michelle Bylicky Jared May C. Norman Coleman Molykutty J. Aryankalayil Whole blood gene expression within days after total-body irradiation predicts long term survival in Gottingen minipigs
description	Abstract Gottingen minipigs mirror the physiological radiation response observed in humans and hence make an ideal candidate model for studying radiation biodosimetry for both limited-sized and mass casualty incidents. We examined the whole blood gene expression profiles starting one day after total-body irradiation with increasing doses of gamma-rays. The minipigs were monitored for up to 45 days or time to euthanasia necessitated by radiation effects. We successfully identified dose- and time-agnostic (over a 1–7 day period after radiation), survival-predictive gene expression signatures derived using machine-learning algorithms with high sensitivity and specificity. These survival-predictive signatures fare better than an optimally performing dose-differentiating signature or blood cellular profiles. These findings suggest that prediction of survival is a much more useful parameter for making triage, resource-utilization and treatment decisions in a resource-constrained environment compared to predictions of total dose received. It should hopefully be possible to build such classifiers for humans in the future.
format	article
author	Sunita Chopra Maria Moroni Jaleal Sanjak Laurel MacMillan Bernadette Hritzo Shannon Martello Michelle Bylicky Jared May C. Norman Coleman Molykutty J. Aryankalayil
author_facet	Sunita Chopra Maria Moroni Jaleal Sanjak Laurel MacMillan Bernadette Hritzo Shannon Martello Michelle Bylicky Jared May C. Norman Coleman Molykutty J. Aryankalayil
author_sort	Sunita Chopra
title	Whole blood gene expression within days after total-body irradiation predicts long term survival in Gottingen minipigs
title_short	Whole blood gene expression within days after total-body irradiation predicts long term survival in Gottingen minipigs
title_full	Whole blood gene expression within days after total-body irradiation predicts long term survival in Gottingen minipigs
title_fullStr	Whole blood gene expression within days after total-body irradiation predicts long term survival in Gottingen minipigs
title_full_unstemmed	Whole blood gene expression within days after total-body irradiation predicts long term survival in Gottingen minipigs
title_sort	whole blood gene expression within days after total-body irradiation predicts long term survival in gottingen minipigs
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a2516ae3c6894857babcede544e3384f
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Whole blood gene expression within days after total-body irradiation predicts long term survival in Gottingen minipigs

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