The proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.

The proteolipid protein (Plp) gene promoter is responsible for driving expression of one of the major components of myelin--PLP and its splice variant DM-20. Both products are classically thought to express predominantly in oligodendrocytes. However, accumulating evidence suggests Plp expression is...

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Autores principales:	John-Paul Michalski, Carrie Anderson, Ariane Beauvais, Yves De Repentigny, Rashmi Kothary
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a251cac494a0407286b85f52f2fcb1ac
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spelling	oai:doaj.org-article:a251cac494a0407286b85f52f2fcb1ac2021-11-18T06:54:12ZThe proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.1932-620310.1371/journal.pone.0019772https://doaj.org/article/a251cac494a0407286b85f52f2fcb1ac2011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21572962/?tool=EBIhttps://doaj.org/toc/1932-6203The proteolipid protein (Plp) gene promoter is responsible for driving expression of one of the major components of myelin--PLP and its splice variant DM-20. Both products are classically thought to express predominantly in oligodendrocytes. However, accumulating evidence suggests Plp expression is more widespread than previously thought. In an attempt to create a mouse model for inducing oligodendrocyte-specific gene deletions, we have generated transgenic mice expressing a Cre recombinase cDNA under control of the mouse Plp promoter. We demonstrate Plp promoter driven Cre expression is restricted predominantly to mature oligodendrocytes of the central nervous system (CNS) at postnatal day 28. However, crosses into the Rosa26(LacZ) and mT/mG reporter mouse lines reveal robust and widespread Cre activity in neuronal tissues at E15.5 and E10.5 that is not strictly oligodendrocyte lineage specific. By P28, all CNS tissues examined displayed high levels of reporter gene expression well outside of defined white matter zones. Importantly, our study reinforces the emerging idea that Plp promoter activity is not restricted to the myelinating cell lineage, but rather, has widespread activity both during embryonic and early postnatal development in the CNS. Specificity of the promoter to the oligodendrocyte cell lineage, as shown through the use of a tamoxifen inducible Plp-CreER(t) line, occurs only at later postnatal stages. Understanding the temporal shift in Plp driven expression is of consequence when designing experimental models to study oligodendrocyte biology.John-Paul MichalskiCarrie AndersonAriane BeauvaisYves De RepentignyRashmi KotharyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19772 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q John-Paul Michalski Carrie Anderson Ariane Beauvais Yves De Repentigny Rashmi Kothary The proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.
description	The proteolipid protein (Plp) gene promoter is responsible for driving expression of one of the major components of myelin--PLP and its splice variant DM-20. Both products are classically thought to express predominantly in oligodendrocytes. However, accumulating evidence suggests Plp expression is more widespread than previously thought. In an attempt to create a mouse model for inducing oligodendrocyte-specific gene deletions, we have generated transgenic mice expressing a Cre recombinase cDNA under control of the mouse Plp promoter. We demonstrate Plp promoter driven Cre expression is restricted predominantly to mature oligodendrocytes of the central nervous system (CNS) at postnatal day 28. However, crosses into the Rosa26(LacZ) and mT/mG reporter mouse lines reveal robust and widespread Cre activity in neuronal tissues at E15.5 and E10.5 that is not strictly oligodendrocyte lineage specific. By P28, all CNS tissues examined displayed high levels of reporter gene expression well outside of defined white matter zones. Importantly, our study reinforces the emerging idea that Plp promoter activity is not restricted to the myelinating cell lineage, but rather, has widespread activity both during embryonic and early postnatal development in the CNS. Specificity of the promoter to the oligodendrocyte cell lineage, as shown through the use of a tamoxifen inducible Plp-CreER(t) line, occurs only at later postnatal stages. Understanding the temporal shift in Plp driven expression is of consequence when designing experimental models to study oligodendrocyte biology.
format	article
author	John-Paul Michalski Carrie Anderson Ariane Beauvais Yves De Repentigny Rashmi Kothary
author_facet	John-Paul Michalski Carrie Anderson Ariane Beauvais Yves De Repentigny Rashmi Kothary
author_sort	John-Paul Michalski
title	The proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.
title_short	The proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.
title_full	The proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.
title_fullStr	The proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.
title_full_unstemmed	The proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.
title_sort	proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a251cac494a0407286b85f52f2fcb1ac
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The proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.

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