A ROLE FOR INTERLEUKIN 8 IN DIRECT REGULATION OF T CELL FUNCTIONAL ACTIVITY

CD3+T lymphocytes were isolated from normal donors by positive magnetic separation. Activation of the T cells with particles conjugated with antibodies to CD3, СD28 and СD2 molecules led to substantial increase in T cell production of interleukin-8 (IL-8). An interleukin-8 receptor (CXCR1, CD181) wa...

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Autores principales:	M. E. Meniailo, V. V. Malashchenko, V. A. Shmarov, N. D. Gasatova, O. B. Melashchenko, A. G. Goncharov, G. V. Seledtsova, V. I. Seledtsov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2017
Materias:	interleukin-8 t lymphocytes interleukin 8 receptor activation of lymphocytes cytokines functional activity Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/a259c0acb997405daa55d701e515dc9d
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spelling	oai:doaj.org-article:a259c0acb997405daa55d701e515dc9d2021-11-18T08:03:46ZA ROLE FOR INTERLEUKIN 8 IN DIRECT REGULATION OF T CELL FUNCTIONAL ACTIVITY1563-06252313-741X10.15789/1563-0625-2017-5-529-536https://doaj.org/article/a259c0acb997405daa55d701e515dc9d2017-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1352https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XCD3+T lymphocytes were isolated from normal donors by positive magnetic separation. Activation of the T cells with particles conjugated with antibodies to CD3, СD28 and СD2 molecules led to substantial increase in T cell production of interleukin-8 (IL-8). An interleukin-8 receptor (CXCR1, CD181) was initially expressed in 13.3% of T lymphocytes. Activation of T lymphocytes resulted into a detectable increase of CD181+ cell number among CD4+ naïve cells and CD4+ terminally-differentiated effector cells, and, conversely, into decrease of their number among CD4+ effector memory cells. Activation of T lymphocytes was assessed by membrane expression of CD25 molecule (receptor for IL-2). IL-8 (0.01-10.0 ng/ml) was shown to markedly reduce activation of both CD4- and CD4+ effector memory T cells, as well as terminallydifferentiated T effectors, without significantly affecting activation of naive T lymphocytes and central memory T cells. IL-8 noticeably increased IL-2 production by activated Т cells, caused a reduced IL-10 production, and did not significantly affect the secretion of IFNγ and IL-4. The data obtained suggest a significance of IL-8 for direct regulation of adaptive T cell responses.M. E. MeniailoV. V. MalashchenkoV. A. ShmarovN. D. GasatovaO. B. MelashchenkoA. G. GoncharovG. V. SeledtsovaV. I. SeledtsovSPb RAACIarticleinterleukin-8t lymphocytesinterleukin 8 receptoractivation of lymphocytescytokinesfunctional activityImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 19, Iss 5, Pp 529-536 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	interleukin-8 t lymphocytes interleukin 8 receptor activation of lymphocytes cytokines functional activity Immunologic diseases. Allergy RC581-607
spellingShingle	interleukin-8 t lymphocytes interleukin 8 receptor activation of lymphocytes cytokines functional activity Immunologic diseases. Allergy RC581-607 M. E. Meniailo V. V. Malashchenko V. A. Shmarov N. D. Gasatova O. B. Melashchenko A. G. Goncharov G. V. Seledtsova V. I. Seledtsov A ROLE FOR INTERLEUKIN 8 IN DIRECT REGULATION OF T CELL FUNCTIONAL ACTIVITY
description	CD3+T lymphocytes were isolated from normal donors by positive magnetic separation. Activation of the T cells with particles conjugated with antibodies to CD3, СD28 and СD2 molecules led to substantial increase in T cell production of interleukin-8 (IL-8). An interleukin-8 receptor (CXCR1, CD181) was initially expressed in 13.3% of T lymphocytes. Activation of T lymphocytes resulted into a detectable increase of CD181+ cell number among CD4+ naïve cells and CD4+ terminally-differentiated effector cells, and, conversely, into decrease of their number among CD4+ effector memory cells. Activation of T lymphocytes was assessed by membrane expression of CD25 molecule (receptor for IL-2). IL-8 (0.01-10.0 ng/ml) was shown to markedly reduce activation of both CD4- and CD4+ effector memory T cells, as well as terminallydifferentiated T effectors, without significantly affecting activation of naive T lymphocytes and central memory T cells. IL-8 noticeably increased IL-2 production by activated Т cells, caused a reduced IL-10 production, and did not significantly affect the secretion of IFNγ and IL-4. The data obtained suggest a significance of IL-8 for direct regulation of adaptive T cell responses.
format	article
author	M. E. Meniailo V. V. Malashchenko V. A. Shmarov N. D. Gasatova O. B. Melashchenko A. G. Goncharov G. V. Seledtsova V. I. Seledtsov
author_facet	M. E. Meniailo V. V. Malashchenko V. A. Shmarov N. D. Gasatova O. B. Melashchenko A. G. Goncharov G. V. Seledtsova V. I. Seledtsov
author_sort	M. E. Meniailo
title	A ROLE FOR INTERLEUKIN 8 IN DIRECT REGULATION OF T CELL FUNCTIONAL ACTIVITY
title_short	A ROLE FOR INTERLEUKIN 8 IN DIRECT REGULATION OF T CELL FUNCTIONAL ACTIVITY
title_full	A ROLE FOR INTERLEUKIN 8 IN DIRECT REGULATION OF T CELL FUNCTIONAL ACTIVITY
title_fullStr	A ROLE FOR INTERLEUKIN 8 IN DIRECT REGULATION OF T CELL FUNCTIONAL ACTIVITY
title_full_unstemmed	A ROLE FOR INTERLEUKIN 8 IN DIRECT REGULATION OF T CELL FUNCTIONAL ACTIVITY
title_sort	role for interleukin 8 in direct regulation of t cell functional activity
publisher	SPb RAACI
publishDate	2017
url	https://doaj.org/article/a259c0acb997405daa55d701e515dc9d
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A ROLE FOR INTERLEUKIN 8 IN DIRECT REGULATION OF T CELL FUNCTIONAL ACTIVITY

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