Rosettes integrity protects Plasmodium vivax of being phagocytized

Abstract Plasmodium vivax is the most prevalent cause of malaria outside of Africa. P. vivax biology and pathogenesis are still poorly understood. The role of one highly occurring phenotype in particular where infected reticulocytes cytoadhere to noninfected normocytes, forming rosettes, remains unk...

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Autores principales:	Letusa Albrecht, Stefanie C. P. Lopes, Ana Beatriz Iung Enembreck da Silva, Vanessa Barbosa, Rodrigo P. Almeida, André M. Siqueira, Juliana Almeida Leite, Najara C. Bittencourt, Hellen Geremias dos Santos, Catarina Bourgard, Luiz Fernando Cardoso Garcia, Ana Carolina A. V. Kayano, Irene S. Soares, Bruce Russell, Laurent Rénia, Marcus V. G. Lacerda, Fabio T. M. Costa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a25cd4efbe0249319d4510263d0d6bd9
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spelling	oai:doaj.org-article:a25cd4efbe0249319d4510263d0d6bd92021-12-02T18:37:07ZRosettes integrity protects Plasmodium vivax of being phagocytized10.1038/s41598-020-73713-w2045-2322https://doaj.org/article/a25cd4efbe0249319d4510263d0d6bd92020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73713-whttps://doaj.org/toc/2045-2322Abstract Plasmodium vivax is the most prevalent cause of malaria outside of Africa. P. vivax biology and pathogenesis are still poorly understood. The role of one highly occurring phenotype in particular where infected reticulocytes cytoadhere to noninfected normocytes, forming rosettes, remains unknown. Here, using a range of ex vivo approaches, we showed that P. vivax rosetting rates were enhanced by plasma of infected patients and that total immunoglobulin M levels correlated with rosetting frequency. Moreover, rosetting rates were also correlated with parasitemia, IL-6 and IL-10 levels in infected patients. Transcriptomic analysis of peripheral leukocytes from P. vivax-infected patients with low or moderated rosetting rates identified differentially expressed genes related to human host phagocytosis pathway. In addition, phagocytosis assay showed that rosetting parasites were less phagocyted. Collectively, these results showed that rosette formation plays a role in host immune response by hampering leukocyte phagocytosis. Thus, these findings suggest that rosetting could be an effective P. vivax immune evasion strategy.Letusa AlbrechtStefanie C. P. LopesAna Beatriz Iung Enembreck da SilvaVanessa BarbosaRodrigo P. AlmeidaAndré M. SiqueiraJuliana Almeida LeiteNajara C. BittencourtHellen Geremias dos SantosCatarina BourgardLuiz Fernando Cardoso GarciaAna Carolina A. V. KayanoIrene S. SoaresBruce RussellLaurent RéniaMarcus V. G. LacerdaFabio T. M. CostaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Letusa Albrecht Stefanie C. P. Lopes Ana Beatriz Iung Enembreck da Silva Vanessa Barbosa Rodrigo P. Almeida André M. Siqueira Juliana Almeida Leite Najara C. Bittencourt Hellen Geremias dos Santos Catarina Bourgard Luiz Fernando Cardoso Garcia Ana Carolina A. V. Kayano Irene S. Soares Bruce Russell Laurent Rénia Marcus V. G. Lacerda Fabio T. M. Costa Rosettes integrity protects Plasmodium vivax of being phagocytized
description	Abstract Plasmodium vivax is the most prevalent cause of malaria outside of Africa. P. vivax biology and pathogenesis are still poorly understood. The role of one highly occurring phenotype in particular where infected reticulocytes cytoadhere to noninfected normocytes, forming rosettes, remains unknown. Here, using a range of ex vivo approaches, we showed that P. vivax rosetting rates were enhanced by plasma of infected patients and that total immunoglobulin M levels correlated with rosetting frequency. Moreover, rosetting rates were also correlated with parasitemia, IL-6 and IL-10 levels in infected patients. Transcriptomic analysis of peripheral leukocytes from P. vivax-infected patients with low or moderated rosetting rates identified differentially expressed genes related to human host phagocytosis pathway. In addition, phagocytosis assay showed that rosetting parasites were less phagocyted. Collectively, these results showed that rosette formation plays a role in host immune response by hampering leukocyte phagocytosis. Thus, these findings suggest that rosetting could be an effective P. vivax immune evasion strategy.
format	article
author	Letusa Albrecht Stefanie C. P. Lopes Ana Beatriz Iung Enembreck da Silva Vanessa Barbosa Rodrigo P. Almeida André M. Siqueira Juliana Almeida Leite Najara C. Bittencourt Hellen Geremias dos Santos Catarina Bourgard Luiz Fernando Cardoso Garcia Ana Carolina A. V. Kayano Irene S. Soares Bruce Russell Laurent Rénia Marcus V. G. Lacerda Fabio T. M. Costa
author_facet	Letusa Albrecht Stefanie C. P. Lopes Ana Beatriz Iung Enembreck da Silva Vanessa Barbosa Rodrigo P. Almeida André M. Siqueira Juliana Almeida Leite Najara C. Bittencourt Hellen Geremias dos Santos Catarina Bourgard Luiz Fernando Cardoso Garcia Ana Carolina A. V. Kayano Irene S. Soares Bruce Russell Laurent Rénia Marcus V. G. Lacerda Fabio T. M. Costa
author_sort	Letusa Albrecht
title	Rosettes integrity protects Plasmodium vivax of being phagocytized
title_short	Rosettes integrity protects Plasmodium vivax of being phagocytized
title_full	Rosettes integrity protects Plasmodium vivax of being phagocytized
title_fullStr	Rosettes integrity protects Plasmodium vivax of being phagocytized
title_full_unstemmed	Rosettes integrity protects Plasmodium vivax of being phagocytized
title_sort	rosettes integrity protects plasmodium vivax of being phagocytized
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a25cd4efbe0249319d4510263d0d6bd9
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Rosettes integrity protects Plasmodium vivax of being phagocytized

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