Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

Abstract Long-term recurrent stress is a common cause of neuropsychiatric disorders. Animal models are widely used to study the pathogenesis of stress-related psychiatric disorders. The zebrafish (Danio rerio) is emerging as a powerful tool to study chronic stress and its mechanisms. Here, we develo...

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Autores principales:	Konstantin A. Demin, Tatiana O. Kolesnikova, David S. Galstyan, Nataliya A. Krotova, Nikita P. Ilyin, Ksenia A. Derzhavina, Nataliia A. Levchenko, Tatyana Strekalova, Murilo S. de Abreu, Elena V. Petersen, Maria Seredinskaya, Yulia V. Cherneyko, Yuriy M. Kositsyn, Dmitry V. Sorokin, Konstantin N. Zabegalov, Mikael S. Mor, Evgeniya V. Efimova, Allan V. Kalueff
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2600ad37ab04fc5a6bf5d176c4f0804
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spelling	oai:doaj.org-article:a2600ad37ab04fc5a6bf5d176c4f08042021-12-02T18:30:39ZModulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model10.1038/s41598-021-92422-62045-2322https://doaj.org/article/a2600ad37ab04fc5a6bf5d176c4f08042021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92422-6https://doaj.org/toc/2045-2322Abstract Long-term recurrent stress is a common cause of neuropsychiatric disorders. Animal models are widely used to study the pathogenesis of stress-related psychiatric disorders. The zebrafish (Danio rerio) is emerging as a powerful tool to study chronic stress and its mechanisms. Here, we developed a prolonged 11-week chronic unpredictable stress (PCUS) model in zebrafish to more fully mimic chronic stress in human populations. We also examined behavioral and neurochemical alterations in zebrafish, and attempted to modulate these states by 3-week treatment with an antidepressant fluoxetine, a neuroprotective omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA), a pro-inflammatory endotoxin lipopolysaccharide (LPS), and their combinations. Overall, PCUS induced severe anxiety and elevated norepinephrine levels, whereas fluoxetine (alone or combined with other agents) corrected most of these behavioral deficits. While EPA and LPS alone had little effects on the zebrafish PCUS-induced anxiety behavior, both fluoxetine (alone or in combination) and EPA restored norepinephrine levels, whereas LPS + EPA increased dopamine levels. As these data support the validity of PCUS as an effective tool to study stress-related pathologies in zebrafish, further research is needed into the ability of various conventional and novel treatments to modulate behavioral and neurochemical biomarkers of chronic stress in this model organism.Konstantin A. DeminTatiana O. KolesnikovaDavid S. GalstyanNataliya A. KrotovaNikita P. IlyinKsenia A. DerzhavinaNataliia A. LevchenkoTatyana StrekalovaMurilo S. de AbreuElena V. PetersenMaria SeredinskayaYulia V. CherneykoYuriy M. KositsynDmitry V. SorokinKonstantin N. ZabegalovMikael S. MorEvgeniya V. EfimovaAllan V. KalueffNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Konstantin A. Demin Tatiana O. Kolesnikova David S. Galstyan Nataliya A. Krotova Nikita P. Ilyin Ksenia A. Derzhavina Nataliia A. Levchenko Tatyana Strekalova Murilo S. de Abreu Elena V. Petersen Maria Seredinskaya Yulia V. Cherneyko Yuriy M. Kositsyn Dmitry V. Sorokin Konstantin N. Zabegalov Mikael S. Mor Evgeniya V. Efimova Allan V. Kalueff Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model
description	Abstract Long-term recurrent stress is a common cause of neuropsychiatric disorders. Animal models are widely used to study the pathogenesis of stress-related psychiatric disorders. The zebrafish (Danio rerio) is emerging as a powerful tool to study chronic stress and its mechanisms. Here, we developed a prolonged 11-week chronic unpredictable stress (PCUS) model in zebrafish to more fully mimic chronic stress in human populations. We also examined behavioral and neurochemical alterations in zebrafish, and attempted to modulate these states by 3-week treatment with an antidepressant fluoxetine, a neuroprotective omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA), a pro-inflammatory endotoxin lipopolysaccharide (LPS), and their combinations. Overall, PCUS induced severe anxiety and elevated norepinephrine levels, whereas fluoxetine (alone or combined with other agents) corrected most of these behavioral deficits. While EPA and LPS alone had little effects on the zebrafish PCUS-induced anxiety behavior, both fluoxetine (alone or in combination) and EPA restored norepinephrine levels, whereas LPS + EPA increased dopamine levels. As these data support the validity of PCUS as an effective tool to study stress-related pathologies in zebrafish, further research is needed into the ability of various conventional and novel treatments to modulate behavioral and neurochemical biomarkers of chronic stress in this model organism.
format	article
author	Konstantin A. Demin Tatiana O. Kolesnikova David S. Galstyan Nataliya A. Krotova Nikita P. Ilyin Ksenia A. Derzhavina Nataliia A. Levchenko Tatyana Strekalova Murilo S. de Abreu Elena V. Petersen Maria Seredinskaya Yulia V. Cherneyko Yuriy M. Kositsyn Dmitry V. Sorokin Konstantin N. Zabegalov Mikael S. Mor Evgeniya V. Efimova Allan V. Kalueff
author_facet	Konstantin A. Demin Tatiana O. Kolesnikova David S. Galstyan Nataliya A. Krotova Nikita P. Ilyin Ksenia A. Derzhavina Nataliia A. Levchenko Tatyana Strekalova Murilo S. de Abreu Elena V. Petersen Maria Seredinskaya Yulia V. Cherneyko Yuriy M. Kositsyn Dmitry V. Sorokin Konstantin N. Zabegalov Mikael S. Mor Evgeniya V. Efimova Allan V. Kalueff
author_sort	Konstantin A. Demin
title	Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model
title_short	Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model
title_full	Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model
title_fullStr	Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model
title_full_unstemmed	Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model
title_sort	modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a2600ad37ab04fc5a6bf5d176c4f0804
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Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

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