A functional requirement for sex-determination M/m locus region lncRNA genes in Aedes aegypti female larvae

Abstract Although many putative long non-coding RNA (lncRNA) genes have been identified in insect genomes, few of these genes have been functionally validated. A screen for female-specific larvicides that facilitate Aedes aegypti male sex separation uncovered multiple interfering RNAs with target si...

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Autores principales:	Keshava Mysore, Limb K. Hapairai, Ping Li, Joseph B. Roethele, Longhua Sun, Jessica Igiede, Joi K. Misenti, Molly Duman-Scheel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a261c85d9dac4d6cbf553474beadabb1
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spelling	oai:doaj.org-article:a261c85d9dac4d6cbf553474beadabb12021-12-02T15:53:01ZA functional requirement for sex-determination M/m locus region lncRNA genes in Aedes aegypti female larvae10.1038/s41598-021-90194-72045-2322https://doaj.org/article/a261c85d9dac4d6cbf553474beadabb12021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90194-7https://doaj.org/toc/2045-2322Abstract Although many putative long non-coding RNA (lncRNA) genes have been identified in insect genomes, few of these genes have been functionally validated. A screen for female-specific larvicides that facilitate Aedes aegypti male sex separation uncovered multiple interfering RNAs with target sites in lncRNA genes located in the M/m locus region, including loci within or tightly linked to the sex determination locus. Larval consumption of a Saccharomyces cerevisiae (yeast) strain engineered to express interfering RNA corresponding to lncRNA transcripts resulted in significant female death, yet had no impact on male survival or fitness. Incorporation of the yeast larvicides into mass culturing protocols facilitated scaled production and separation of fit adult males, indicating that yeast larvicides could benefit mosquito population control strategies that rely on mass releases of male mosquitoes. These studies functionally verified a female-specific developmental requirement for M/m locus region lncRNA genes, suggesting that sexually antagonistic lncRNA genes found within this highly repetitive pericentromeric DNA sequence may be contributing to the evolution of A. aegypti sex chromosomes.Keshava MysoreLimb K. HapairaiPing LiJoseph B. RoetheleLonghua SunJessica IgiedeJoi K. MisentiMolly Duman-ScheelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keshava Mysore Limb K. Hapairai Ping Li Joseph B. Roethele Longhua Sun Jessica Igiede Joi K. Misenti Molly Duman-Scheel A functional requirement for sex-determination M/m locus region lncRNA genes in Aedes aegypti female larvae
description	Abstract Although many putative long non-coding RNA (lncRNA) genes have been identified in insect genomes, few of these genes have been functionally validated. A screen for female-specific larvicides that facilitate Aedes aegypti male sex separation uncovered multiple interfering RNAs with target sites in lncRNA genes located in the M/m locus region, including loci within or tightly linked to the sex determination locus. Larval consumption of a Saccharomyces cerevisiae (yeast) strain engineered to express interfering RNA corresponding to lncRNA transcripts resulted in significant female death, yet had no impact on male survival or fitness. Incorporation of the yeast larvicides into mass culturing protocols facilitated scaled production and separation of fit adult males, indicating that yeast larvicides could benefit mosquito population control strategies that rely on mass releases of male mosquitoes. These studies functionally verified a female-specific developmental requirement for M/m locus region lncRNA genes, suggesting that sexually antagonistic lncRNA genes found within this highly repetitive pericentromeric DNA sequence may be contributing to the evolution of A. aegypti sex chromosomes.
format	article
author	Keshava Mysore Limb K. Hapairai Ping Li Joseph B. Roethele Longhua Sun Jessica Igiede Joi K. Misenti Molly Duman-Scheel
author_facet	Keshava Mysore Limb K. Hapairai Ping Li Joseph B. Roethele Longhua Sun Jessica Igiede Joi K. Misenti Molly Duman-Scheel
author_sort	Keshava Mysore
title	A functional requirement for sex-determination M/m locus region lncRNA genes in Aedes aegypti female larvae
title_short	A functional requirement for sex-determination M/m locus region lncRNA genes in Aedes aegypti female larvae
title_full	A functional requirement for sex-determination M/m locus region lncRNA genes in Aedes aegypti female larvae
title_fullStr	A functional requirement for sex-determination M/m locus region lncRNA genes in Aedes aegypti female larvae
title_full_unstemmed	A functional requirement for sex-determination M/m locus region lncRNA genes in Aedes aegypti female larvae
title_sort	functional requirement for sex-determination m/m locus region lncrna genes in aedes aegypti female larvae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a261c85d9dac4d6cbf553474beadabb1
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A functional requirement for sex-determination M/m locus region lncRNA genes in Aedes aegypti female larvae

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