Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin

Eulalia Sans-Serramitjana,1 Marta Jorba,1 José Luis Pedraz,2 Teresa Vinuesa,1 Miguel Viñas1 1Laboratory of Molecular Microbiology and Antimicrobials, Department of Pathology and Experimental Therapeutics, University of Barcelona, Barcelona, 2Laboratory of Pharmaceutics, Univer...

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Autores principales:	Sans-Serramitjana E, Jorba M, Pedraz JL, Vinuesa T, Viñas M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	cystic fibrosis colistin sulfate lipid nanoparticles (NLC) P. aeruginosa confocal laser scanning microscopy anti-biofilm activity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a2650a8f626a432ebc66d2d7c942e851
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spelling	oai:doaj.org-article:a2650a8f626a432ebc66d2d7c942e8512021-12-02T01:55:20ZDetermination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin1178-2013https://doaj.org/article/a2650a8f626a432ebc66d2d7c942e8512017-06-01T00:00:00Zhttps://www.dovepress.com/determination-of-the-spatiotemporal-dependence-of-pseudomonas-aerugino-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Eulalia Sans-Serramitjana,1 Marta Jorba,1 José Luis Pedraz,2 Teresa Vinuesa,1 Miguel Viñas1 1Laboratory of Molecular Microbiology and Antimicrobials, Department of Pathology and Experimental Therapeutics, University of Barcelona, Barcelona, 2Laboratory of Pharmaceutics, University of the Basque Country (UPV/EHU), Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina, Vitoria, Spain Abstract: The emergence of colistin-resistant Pseudomonas aeruginosa in cystic fibrosis (CF) patients, particularly after long-term inhalation treatments, has been recently reported. Nanoencapsulation may enable preparations to overcome the limitations of conventional pharmaceutical forms. We have determined the time-dependent viability of P. aeruginosa biofilms treated with both free and nanoencapsulated colistin. We also examined the relationship between the optimal anti-biofilm activity of nanostructured lipid carrier (NLC)-colistin and the structural organization of the biofilm itself. The results showed the more rapid killing of P. aeruginosa bacterial biofilms by NLC-colistin than by free colistin. However, the two formulations did not differ in terms of the final percentages of living and dead cells, which were higher in the inner than in the outer layers of the treated biofilms. The effective anti-biofilm activity of NLC-colistin and its faster killing effect recommend further studies of its use over free colistin in the treatment of P. aeruginosa infections in CF patients. Keywords: cystic fibrosis, colistin sulfate, lipid nanoparticles, P. aeruginosa, confocal laser scanning microscopy, anti-biofilm activitySans-Serramitjana EJorba MPedraz JLVinuesa TViñas MDove Medical Pressarticlecystic fibrosiscolistin sulfatelipid nanoparticles (NLC)P. aeruginosaconfocal laser scanning microscopyanti-biofilm activityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 4409-4413 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	cystic fibrosis colistin sulfate lipid nanoparticles (NLC) P. aeruginosa confocal laser scanning microscopy anti-biofilm activity Medicine (General) R5-920
spellingShingle	cystic fibrosis colistin sulfate lipid nanoparticles (NLC) P. aeruginosa confocal laser scanning microscopy anti-biofilm activity Medicine (General) R5-920 Sans-Serramitjana E Jorba M Pedraz JL Vinuesa T Viñas M Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin
description	Eulalia Sans-Serramitjana,1 Marta Jorba,1 José Luis Pedraz,2 Teresa Vinuesa,1 Miguel Viñas1 1Laboratory of Molecular Microbiology and Antimicrobials, Department of Pathology and Experimental Therapeutics, University of Barcelona, Barcelona, 2Laboratory of Pharmaceutics, University of the Basque Country (UPV/EHU), Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina, Vitoria, Spain Abstract: The emergence of colistin-resistant Pseudomonas aeruginosa in cystic fibrosis (CF) patients, particularly after long-term inhalation treatments, has been recently reported. Nanoencapsulation may enable preparations to overcome the limitations of conventional pharmaceutical forms. We have determined the time-dependent viability of P. aeruginosa biofilms treated with both free and nanoencapsulated colistin. We also examined the relationship between the optimal anti-biofilm activity of nanostructured lipid carrier (NLC)-colistin and the structural organization of the biofilm itself. The results showed the more rapid killing of P. aeruginosa bacterial biofilms by NLC-colistin than by free colistin. However, the two formulations did not differ in terms of the final percentages of living and dead cells, which were higher in the inner than in the outer layers of the treated biofilms. The effective anti-biofilm activity of NLC-colistin and its faster killing effect recommend further studies of its use over free colistin in the treatment of P. aeruginosa infections in CF patients. Keywords: cystic fibrosis, colistin sulfate, lipid nanoparticles, P. aeruginosa, confocal laser scanning microscopy, anti-biofilm activity
format	article
author	Sans-Serramitjana E Jorba M Pedraz JL Vinuesa T Viñas M
author_facet	Sans-Serramitjana E Jorba M Pedraz JL Vinuesa T Viñas M
author_sort	Sans-Serramitjana E
title	Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin
title_short	Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin
title_full	Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin
title_fullStr	Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin
title_full_unstemmed	Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin
title_sort	determination of the spatiotemporal dependence of pseudomonas aeruginosa biofilm viability after treatment with nlc-colistin
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/a2650a8f626a432ebc66d2d7c942e851
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Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin

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