Peptide-based scaffolds for the culture and maintenance of primary human hepatocytes

Abstract We report here the use of a nanofibrous hydrogel as a 3D scaffold for the culture and maintenance of functional primary human hepatocytes. The system is based on the cooperative assembly of a fiber-forming peptide component, fluorenylmethyloxycarbonyl-diphenylalanine (Fmoc-FF), and the inte...

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Autores principales:	Douglas MacPherson, Yaron Bram, Jiwoon Park, Robert E. Schwartz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a265405ebb39499f8c65ae9cd5b1da6b
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spelling	oai:doaj.org-article:a265405ebb39499f8c65ae9cd5b1da6b2021-12-02T13:24:15ZPeptide-based scaffolds for the culture and maintenance of primary human hepatocytes10.1038/s41598-021-86016-52045-2322https://doaj.org/article/a265405ebb39499f8c65ae9cd5b1da6b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86016-5https://doaj.org/toc/2045-2322Abstract We report here the use of a nanofibrous hydrogel as a 3D scaffold for the culture and maintenance of functional primary human hepatocytes. The system is based on the cooperative assembly of a fiber-forming peptide component, fluorenylmethyloxycarbonyl-diphenylalanine (Fmoc-FF), and the integrin-binding functional peptide ligand, Fmoc-arginine-glycine-aspartic acid (Fmoc-RGD) into a nanofibrous gel at physiological pH. This Fmoc-FF/RGD hydrogel was formulated to provide a biomimetic microenvironment with some critical features such as mechanical properties and nanofiber morphology, which were optimized to support hepatocyte culture. The material was shown to support maintenance and function of encapsulated primary human hepatocytes as indicated by actin staining, qRT-PCR, and functional cytochrome P450 assays. The designed gel was shown to outperform Matrigel in cytochrome P450 functional assays. The hydrogel may prove useful for liver development and disease models, as well as providing insights into the design of future implantable scaffolds for the regeneration of liver tissue in patients with liver disease.Douglas MacPhersonYaron BramJiwoon ParkRobert E. SchwartzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Douglas MacPherson Yaron Bram Jiwoon Park Robert E. Schwartz Peptide-based scaffolds for the culture and maintenance of primary human hepatocytes
description	Abstract We report here the use of a nanofibrous hydrogel as a 3D scaffold for the culture and maintenance of functional primary human hepatocytes. The system is based on the cooperative assembly of a fiber-forming peptide component, fluorenylmethyloxycarbonyl-diphenylalanine (Fmoc-FF), and the integrin-binding functional peptide ligand, Fmoc-arginine-glycine-aspartic acid (Fmoc-RGD) into a nanofibrous gel at physiological pH. This Fmoc-FF/RGD hydrogel was formulated to provide a biomimetic microenvironment with some critical features such as mechanical properties and nanofiber morphology, which were optimized to support hepatocyte culture. The material was shown to support maintenance and function of encapsulated primary human hepatocytes as indicated by actin staining, qRT-PCR, and functional cytochrome P450 assays. The designed gel was shown to outperform Matrigel in cytochrome P450 functional assays. The hydrogel may prove useful for liver development and disease models, as well as providing insights into the design of future implantable scaffolds for the regeneration of liver tissue in patients with liver disease.
format	article
author	Douglas MacPherson Yaron Bram Jiwoon Park Robert E. Schwartz
author_facet	Douglas MacPherson Yaron Bram Jiwoon Park Robert E. Schwartz
author_sort	Douglas MacPherson
title	Peptide-based scaffolds for the culture and maintenance of primary human hepatocytes
title_short	Peptide-based scaffolds for the culture and maintenance of primary human hepatocytes
title_full	Peptide-based scaffolds for the culture and maintenance of primary human hepatocytes
title_fullStr	Peptide-based scaffolds for the culture and maintenance of primary human hepatocytes
title_full_unstemmed	Peptide-based scaffolds for the culture and maintenance of primary human hepatocytes
title_sort	peptide-based scaffolds for the culture and maintenance of primary human hepatocytes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a265405ebb39499f8c65ae9cd5b1da6b
work_keys_str_mv	AT douglasmacpherson peptidebasedscaffoldsforthecultureandmaintenanceofprimaryhumanhepatocytes AT yaronbram peptidebasedscaffoldsforthecultureandmaintenanceofprimaryhumanhepatocytes AT jiwoonpark peptidebasedscaffoldsforthecultureandmaintenanceofprimaryhumanhepatocytes AT roberteschwartz peptidebasedscaffoldsforthecultureandmaintenanceofprimaryhumanhepatocytes
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Peptide-based scaffolds for the culture and maintenance of primary human hepatocytes

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