A subset of liver resident natural killer cells is expanded in hepatitis C-infected patients with better liver function
Abstract Viral hepatitis leads to immune-mediated liver injury. The rate of disease progression varies between individuals. We aimed to phenotype immune cells associated with preservation of normal liver function during hepatitis C virus (HCV) infection. Clinical data and specimens were obtained fro...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/a26e3fb007f44f6b928d328f7069b422 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:a26e3fb007f44f6b928d328f7069b422 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:a26e3fb007f44f6b928d328f7069b4222021-12-02T14:01:22ZA subset of liver resident natural killer cells is expanded in hepatitis C-infected patients with better liver function10.1038/s41598-020-80819-82045-2322https://doaj.org/article/a26e3fb007f44f6b928d328f7069b4222021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80819-8https://doaj.org/toc/2045-2322Abstract Viral hepatitis leads to immune-mediated liver injury. The rate of disease progression varies between individuals. We aimed to phenotype immune cells associated with preservation of normal liver function during hepatitis C virus (HCV) infection. Clinical data and specimens were obtained from 19 HCV-infected patients undergoing liver transplantation. Liver and peripheral blood mononuclear cells were isolated and eight subsets of innate immune cells were delineated by multiparameter flow cytometry. Cytokine assays and microarrays were performed. Intrahepatic CD56Bright/CD16- natural killer (NK) cells comprised the only subset correlating with better liver function, i.e., lower bilirubin (p = 0.0002) and lower model for end stage of liver disease scores (p = 0.03). The signature of liver NK cells from HCV-infected patients included genes expressed by NK cells in normal liver and by decidual NK cells. Portal vein blood had a higher concentration of interleukin (IL)-10 than peripheral blood (p = 0.03). LMCs were less responsive to toll-like receptor (TLR) stimulation than PBMCs, with fewer pro-inflammatory gene-expression pathways up-regulated after in vitro exposure to lipopolysaccharide and a TLR-7/8 agonist. Hepatic CD56Bright/CD16- NK cells may be critical for maintaining liver homeostasis. Portal vein IL-10 may prime inhibitory pathways, attenuating TLR signaling and reducing responsiveness to pro-inflammatory stimuli.Erin H. DoyleCostica AlomanAhmed El-ShamyFrancis EngAdeeb RahmanArielle L. KlepperBrandy HaydelSander S. FlormanM. Isabel FielThomas SchianoAndrea D. BranchNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Erin H. Doyle Costica Aloman Ahmed El-Shamy Francis Eng Adeeb Rahman Arielle L. Klepper Brandy Haydel Sander S. Florman M. Isabel Fiel Thomas Schiano Andrea D. Branch A subset of liver resident natural killer cells is expanded in hepatitis C-infected patients with better liver function |
| description |
Abstract Viral hepatitis leads to immune-mediated liver injury. The rate of disease progression varies between individuals. We aimed to phenotype immune cells associated with preservation of normal liver function during hepatitis C virus (HCV) infection. Clinical data and specimens were obtained from 19 HCV-infected patients undergoing liver transplantation. Liver and peripheral blood mononuclear cells were isolated and eight subsets of innate immune cells were delineated by multiparameter flow cytometry. Cytokine assays and microarrays were performed. Intrahepatic CD56Bright/CD16- natural killer (NK) cells comprised the only subset correlating with better liver function, i.e., lower bilirubin (p = 0.0002) and lower model for end stage of liver disease scores (p = 0.03). The signature of liver NK cells from HCV-infected patients included genes expressed by NK cells in normal liver and by decidual NK cells. Portal vein blood had a higher concentration of interleukin (IL)-10 than peripheral blood (p = 0.03). LMCs were less responsive to toll-like receptor (TLR) stimulation than PBMCs, with fewer pro-inflammatory gene-expression pathways up-regulated after in vitro exposure to lipopolysaccharide and a TLR-7/8 agonist. Hepatic CD56Bright/CD16- NK cells may be critical for maintaining liver homeostasis. Portal vein IL-10 may prime inhibitory pathways, attenuating TLR signaling and reducing responsiveness to pro-inflammatory stimuli. |
| format |
article |
| author |
Erin H. Doyle Costica Aloman Ahmed El-Shamy Francis Eng Adeeb Rahman Arielle L. Klepper Brandy Haydel Sander S. Florman M. Isabel Fiel Thomas Schiano Andrea D. Branch |
| author_facet |
Erin H. Doyle Costica Aloman Ahmed El-Shamy Francis Eng Adeeb Rahman Arielle L. Klepper Brandy Haydel Sander S. Florman M. Isabel Fiel Thomas Schiano Andrea D. Branch |
| author_sort |
Erin H. Doyle |
| title |
A subset of liver resident natural killer cells is expanded in hepatitis C-infected patients with better liver function |
| title_short |
A subset of liver resident natural killer cells is expanded in hepatitis C-infected patients with better liver function |
| title_full |
A subset of liver resident natural killer cells is expanded in hepatitis C-infected patients with better liver function |
| title_fullStr |
A subset of liver resident natural killer cells is expanded in hepatitis C-infected patients with better liver function |
| title_full_unstemmed |
A subset of liver resident natural killer cells is expanded in hepatitis C-infected patients with better liver function |
| title_sort |
subset of liver resident natural killer cells is expanded in hepatitis c-infected patients with better liver function |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/a26e3fb007f44f6b928d328f7069b422 |
| work_keys_str_mv |
AT erinhdoyle asubsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT costicaaloman asubsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT ahmedelshamy asubsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT franciseng asubsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT adeebrahman asubsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT ariellelklepper asubsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT brandyhaydel asubsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT sandersflorman asubsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT misabelfiel asubsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT thomasschiano asubsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT andreadbranch asubsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT erinhdoyle subsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT costicaaloman subsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT ahmedelshamy subsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT franciseng subsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT adeebrahman subsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT ariellelklepper subsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT brandyhaydel subsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT sandersflorman subsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT misabelfiel subsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT thomasschiano subsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction AT andreadbranch subsetofliverresidentnaturalkillercellsisexpandedinhepatitiscinfectedpatientswithbetterliverfunction |
| _version_ |
1718392159980224512 |