Molecular characterization of a new highly divergent Mobala related arenavirus isolated from Praomys sp. rodents

Abstract Arenaviruses represent a family of viruses that are naturally present in rodents belonging to subfamily Murinae, Neotominae or Sigmodontinae. Except for Lassa virus, little information is available on other Old-World arenaviruses. Here, we describe strain AnRB3214, a virus isolated from a p...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Huguette Simo Tchetgna, Stephane Descorps-Declère, Benjamin Selekon, Aurelia Kwasiborski, Mathias Vandenbogaert, Jean-Claude Manuguerra, Antoine Gessain, Valérie Caro, Emmanuel Nakouné, Nicolas Berthet
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2703dce209f4f5b8ae73281cb58ec73
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a2703dce209f4f5b8ae73281cb58ec73
record_format	dspace
spelling	oai:doaj.org-article:a2703dce209f4f5b8ae73281cb58ec732021-12-02T15:43:17ZMolecular characterization of a new highly divergent Mobala related arenavirus isolated from Praomys sp. rodents10.1038/s41598-021-88046-52045-2322https://doaj.org/article/a2703dce209f4f5b8ae73281cb58ec732021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88046-5https://doaj.org/toc/2045-2322Abstract Arenaviruses represent a family of viruses that are naturally present in rodents belonging to subfamily Murinae, Neotominae or Sigmodontinae. Except for Lassa virus, little information is available on other Old-World arenaviruses. Here, we describe strain AnRB3214, a virus isolated from a presumed Praomys sp. rodent in the Central African Republic in 1981 and assigned to Ippy virus based on antigenic similarity. The strain was simultaneously sequenced on Illumina NovaSeq 6000 and MinION Mk1B devices and analysed with various bioinformatics tools. We show that the best genome coverage and depth were obtained with the Kaiju and Minimap2 classification and identification tools, on either the MinION or the Illumina reads. The genetic analysis of AnRB3214 fragments showed 68% to 79% similarity with the Mobala and Gairo mammarenaviruses at the nucleic acid level. Strain AnRB3214 had a truncated nucleoprotein smaller than that of other Old World arenaviruses. Molecular clock analysis suggests that this strain diverged from Mobala virus at least 400 years ago. Finally, this study illustrates the importance of genomics in the identification of archived viruses and expands on the diversity of African arenaviruses, because strain AnRB3214 is either a variant or a close relative of Mobala virus, and not Ippy virus.Huguette Simo TchetgnaStephane Descorps-DeclèreBenjamin SelekonAurelia KwasiborskiMathias VandenbogaertJean-Claude ManuguerraAntoine GessainValérie CaroEmmanuel NakounéNicolas BerthetNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Huguette Simo Tchetgna Stephane Descorps-Declère Benjamin Selekon Aurelia Kwasiborski Mathias Vandenbogaert Jean-Claude Manuguerra Antoine Gessain Valérie Caro Emmanuel Nakouné Nicolas Berthet Molecular characterization of a new highly divergent Mobala related arenavirus isolated from Praomys sp. rodents
description	Abstract Arenaviruses represent a family of viruses that are naturally present in rodents belonging to subfamily Murinae, Neotominae or Sigmodontinae. Except for Lassa virus, little information is available on other Old-World arenaviruses. Here, we describe strain AnRB3214, a virus isolated from a presumed Praomys sp. rodent in the Central African Republic in 1981 and assigned to Ippy virus based on antigenic similarity. The strain was simultaneously sequenced on Illumina NovaSeq 6000 and MinION Mk1B devices and analysed with various bioinformatics tools. We show that the best genome coverage and depth were obtained with the Kaiju and Minimap2 classification and identification tools, on either the MinION or the Illumina reads. The genetic analysis of AnRB3214 fragments showed 68% to 79% similarity with the Mobala and Gairo mammarenaviruses at the nucleic acid level. Strain AnRB3214 had a truncated nucleoprotein smaller than that of other Old World arenaviruses. Molecular clock analysis suggests that this strain diverged from Mobala virus at least 400 years ago. Finally, this study illustrates the importance of genomics in the identification of archived viruses and expands on the diversity of African arenaviruses, because strain AnRB3214 is either a variant or a close relative of Mobala virus, and not Ippy virus.
format	article
author	Huguette Simo Tchetgna Stephane Descorps-Declère Benjamin Selekon Aurelia Kwasiborski Mathias Vandenbogaert Jean-Claude Manuguerra Antoine Gessain Valérie Caro Emmanuel Nakouné Nicolas Berthet
author_facet	Huguette Simo Tchetgna Stephane Descorps-Declère Benjamin Selekon Aurelia Kwasiborski Mathias Vandenbogaert Jean-Claude Manuguerra Antoine Gessain Valérie Caro Emmanuel Nakouné Nicolas Berthet
author_sort	Huguette Simo Tchetgna
title	Molecular characterization of a new highly divergent Mobala related arenavirus isolated from Praomys sp. rodents
title_short	Molecular characterization of a new highly divergent Mobala related arenavirus isolated from Praomys sp. rodents
title_full	Molecular characterization of a new highly divergent Mobala related arenavirus isolated from Praomys sp. rodents
title_fullStr	Molecular characterization of a new highly divergent Mobala related arenavirus isolated from Praomys sp. rodents
title_full_unstemmed	Molecular characterization of a new highly divergent Mobala related arenavirus isolated from Praomys sp. rodents
title_sort	molecular characterization of a new highly divergent mobala related arenavirus isolated from praomys sp. rodents
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a2703dce209f4f5b8ae73281cb58ec73
work_keys_str_mv	AT huguettesimotchetgna molecularcharacterizationofanewhighlydivergentmobalarelatedarenavirusisolatedfrompraomyssprodents AT stephanedescorpsdeclere molecularcharacterizationofanewhighlydivergentmobalarelatedarenavirusisolatedfrompraomyssprodents AT benjaminselekon molecularcharacterizationofanewhighlydivergentmobalarelatedarenavirusisolatedfrompraomyssprodents AT aureliakwasiborski molecularcharacterizationofanewhighlydivergentmobalarelatedarenavirusisolatedfrompraomyssprodents AT mathiasvandenbogaert molecularcharacterizationofanewhighlydivergentmobalarelatedarenavirusisolatedfrompraomyssprodents AT jeanclaudemanuguerra molecularcharacterizationofanewhighlydivergentmobalarelatedarenavirusisolatedfrompraomyssprodents AT antoinegessain molecularcharacterizationofanewhighlydivergentmobalarelatedarenavirusisolatedfrompraomyssprodents AT valeriecaro molecularcharacterizationofanewhighlydivergentmobalarelatedarenavirusisolatedfrompraomyssprodents AT emmanuelnakoune molecularcharacterizationofanewhighlydivergentmobalarelatedarenavirusisolatedfrompraomyssprodents AT nicolasberthet molecularcharacterizationofanewhighlydivergentmobalarelatedarenavirusisolatedfrompraomyssprodents
_version_	1718385825571405824

Molecular characterization of a new highly divergent Mobala related arenavirus isolated from Praomys sp. rodents

Ejemplares similares