Detection of herpes simplex virus type-1 in patients with fibrotic lung diseases.

The current study intends to investigate i) the incidence of herpes viruses including Herpes Simplex Virus type-1 (HSV-1), Cytomegalovirus (CMV) and Human Herpes Virus -6, -7, -8 (HHV6, HHV7, HHV8) in two biological samples, bronchoalveolar lavage fluid (BALF) and lung tissue biopsy, in different fo...

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Autores principales:	Ismini Lasithiotaki, Katerina M Antoniou, Virginia-Maria Vlahava, Konstantinos Karagiannis, Demetrios A Spandidos, Nikolaos M Siafakas, George Sourvinos
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a27af50dfdb549d8b8f3534f70cafb56
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spelling	oai:doaj.org-article:a27af50dfdb549d8b8f3534f70cafb562021-11-18T07:31:50ZDetection of herpes simplex virus type-1 in patients with fibrotic lung diseases.1932-620310.1371/journal.pone.0027800https://doaj.org/article/a27af50dfdb549d8b8f3534f70cafb562011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22205929/?tool=EBIhttps://doaj.org/toc/1932-6203The current study intends to investigate i) the incidence of herpes viruses including Herpes Simplex Virus type-1 (HSV-1), Cytomegalovirus (CMV) and Human Herpes Virus -6, -7, -8 (HHV6, HHV7, HHV8) in two biological samples, bronchoalveolar lavage fluid (BALF) and lung tissue biopsy, in different forms of pulmonary fibrosis, and ii) the induction of molecular pathways involved in fibrosis by herpesvirus infection in primary cell cultures. PCR was employed for the detection of CMV, HHV6-8 and HSV-1 DNA in lung specimens (4 controls and 11 IPF specimens) and BALF pellet [6 controls and 20 fibrotic Idiopathic Intestitial Pneumonias (f-IIPs) samples: 13 idiopathic pulmonary fibrosis (IPF) and 7 nonspecific idiopathic interstitial pneumonia (NSIP)] samples. Among all herpesviruses tested, HSV-1 was detected in 1/11 (9%) specimens from IPF lung tissue and in 2/20 (10%) samples of f-IIPs BALF whereas the control group was negative. Primary cell cultures from BALF of patients with IPF and healthy controls were infected in vitro with wild-type HSV-1 virus and Real Time PCR was employed for the detection of gene transcription of specific axes implicated in lung fibrosis. Primary cell cultures were permissive to HSV-1, resulting in an upregulation of the fibrotic growth factors TGFβ1 and FGF, the angiogenetic markers SDF1a, SDF1b, VEGF, FGF and the regulators of tissue wound healing MMP9 and CCR7. Downregulation was noted for the CXCR4 and MMP2 genes, while a different response has been detected in healthy donors regarding the expression of the aforementioned markers. These results implicate for the first time the HSV-1 with Fibrotic Idiopathic Interstitial Pneumonias since the virus presented similar incidence in two different biological samples.Ismini LasithiotakiKaterina M AntoniouVirginia-Maria VlahavaKonstantinos KaragiannisDemetrios A SpandidosNikolaos M SiafakasGeorge SourvinosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e27800 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ismini Lasithiotaki Katerina M Antoniou Virginia-Maria Vlahava Konstantinos Karagiannis Demetrios A Spandidos Nikolaos M Siafakas George Sourvinos Detection of herpes simplex virus type-1 in patients with fibrotic lung diseases.
description	The current study intends to investigate i) the incidence of herpes viruses including Herpes Simplex Virus type-1 (HSV-1), Cytomegalovirus (CMV) and Human Herpes Virus -6, -7, -8 (HHV6, HHV7, HHV8) in two biological samples, bronchoalveolar lavage fluid (BALF) and lung tissue biopsy, in different forms of pulmonary fibrosis, and ii) the induction of molecular pathways involved in fibrosis by herpesvirus infection in primary cell cultures. PCR was employed for the detection of CMV, HHV6-8 and HSV-1 DNA in lung specimens (4 controls and 11 IPF specimens) and BALF pellet [6 controls and 20 fibrotic Idiopathic Intestitial Pneumonias (f-IIPs) samples: 13 idiopathic pulmonary fibrosis (IPF) and 7 nonspecific idiopathic interstitial pneumonia (NSIP)] samples. Among all herpesviruses tested, HSV-1 was detected in 1/11 (9%) specimens from IPF lung tissue and in 2/20 (10%) samples of f-IIPs BALF whereas the control group was negative. Primary cell cultures from BALF of patients with IPF and healthy controls were infected in vitro with wild-type HSV-1 virus and Real Time PCR was employed for the detection of gene transcription of specific axes implicated in lung fibrosis. Primary cell cultures were permissive to HSV-1, resulting in an upregulation of the fibrotic growth factors TGFβ1 and FGF, the angiogenetic markers SDF1a, SDF1b, VEGF, FGF and the regulators of tissue wound healing MMP9 and CCR7. Downregulation was noted for the CXCR4 and MMP2 genes, while a different response has been detected in healthy donors regarding the expression of the aforementioned markers. These results implicate for the first time the HSV-1 with Fibrotic Idiopathic Interstitial Pneumonias since the virus presented similar incidence in two different biological samples.
format	article
author	Ismini Lasithiotaki Katerina M Antoniou Virginia-Maria Vlahava Konstantinos Karagiannis Demetrios A Spandidos Nikolaos M Siafakas George Sourvinos
author_facet	Ismini Lasithiotaki Katerina M Antoniou Virginia-Maria Vlahava Konstantinos Karagiannis Demetrios A Spandidos Nikolaos M Siafakas George Sourvinos
author_sort	Ismini Lasithiotaki
title	Detection of herpes simplex virus type-1 in patients with fibrotic lung diseases.
title_short	Detection of herpes simplex virus type-1 in patients with fibrotic lung diseases.
title_full	Detection of herpes simplex virus type-1 in patients with fibrotic lung diseases.
title_fullStr	Detection of herpes simplex virus type-1 in patients with fibrotic lung diseases.
title_full_unstemmed	Detection of herpes simplex virus type-1 in patients with fibrotic lung diseases.
title_sort	detection of herpes simplex virus type-1 in patients with fibrotic lung diseases.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a27af50dfdb549d8b8f3534f70cafb56
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Detection of herpes simplex virus type-1 in patients with fibrotic lung diseases.

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