Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast

Construction of yeast libraries is time-consuming, costly and limited to the genetic background of the chosen strain. Here the authors present CASTLING which uses CRISPR-Cas12a and oligonucleotide pools to rapidly generate pooled libraries with large insertions such as fluorescent protein tags.

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Autores principales:	Benjamin C. Buchmuller, Konrad Herbst, Matthias Meurer, Daniel Kirrmaier, Ehud Sass, Emmanuel D. Levy, Michael Knop
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a27b637012fa456695e303da14f899ec
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spelling	oai:doaj.org-article:a27b637012fa456695e303da14f899ec2021-12-02T17:31:53ZPooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast10.1038/s41467-019-10816-72041-1723https://doaj.org/article/a27b637012fa456695e303da14f899ec2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10816-7https://doaj.org/toc/2041-1723Construction of yeast libraries is time-consuming, costly and limited to the genetic background of the chosen strain. Here the authors present CASTLING which uses CRISPR-Cas12a and oligonucleotide pools to rapidly generate pooled libraries with large insertions such as fluorescent protein tags.Benjamin C. BuchmullerKonrad HerbstMatthias MeurerDaniel KirrmaierEhud SassEmmanuel D. LevyMichael KnopNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Benjamin C. Buchmuller Konrad Herbst Matthias Meurer Daniel Kirrmaier Ehud Sass Emmanuel D. Levy Michael Knop Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast
description	Construction of yeast libraries is time-consuming, costly and limited to the genetic background of the chosen strain. Here the authors present CASTLING which uses CRISPR-Cas12a and oligonucleotide pools to rapidly generate pooled libraries with large insertions such as fluorescent protein tags.
format	article
author	Benjamin C. Buchmuller Konrad Herbst Matthias Meurer Daniel Kirrmaier Ehud Sass Emmanuel D. Levy Michael Knop
author_facet	Benjamin C. Buchmuller Konrad Herbst Matthias Meurer Daniel Kirrmaier Ehud Sass Emmanuel D. Levy Michael Knop
author_sort	Benjamin C. Buchmuller
title	Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast
title_short	Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast
title_full	Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast
title_fullStr	Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast
title_full_unstemmed	Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast
title_sort	pooled clone collections by multiplexed crispr-cas12a-assisted gene tagging in yeast
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/a27b637012fa456695e303da14f899ec
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Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast

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