Nanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors

Mateusz Cieśluk,1 Katarzyna Pogoda,2 Piotr Deptuła,1 Paulina Werel,3 Alina Kułakowska,3 Jan Kochanowicz,3 Zenon Mariak,4 Tomasz Łysoń,4 Joanna Reszeć,5 Robert Bucki1 1Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Bialystok PL-15222, Poland; 2Inst...

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Autores principales:	Cieśluk M, Pogoda K, Deptuła P, Werel P, Kułakowska A, Kochanowicz J, Mariak Z, Łysoń T, Reszeć J, Bucki R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	afm brain tumors human tissue rheology mechanomarkers glioblastoma Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a27fb3e5b71c42ce84ee6df823fd524b
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spelling	oai:doaj.org-article:a27fb3e5b71c42ce84ee6df823fd524b2021-12-02T15:28:30ZNanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors1178-2013https://doaj.org/article/a27fb3e5b71c42ce84ee6df823fd524b2020-10-01T00:00:00Zhttps://www.dovepress.com/nanomechanics-and-histopathology-as-diagnostic-tools-to-characterize-f-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mateusz Cieśluk,1 Katarzyna Pogoda,2 Piotr Deptuła,1 Paulina Werel,3 Alina Kułakowska,3 Jan Kochanowicz,3 Zenon Mariak,4 Tomasz Łysoń,4 Joanna Reszeć,5 Robert Bucki1 1Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Bialystok PL-15222, Poland; 2Institute of Nuclear Physics, Polish Academy of Sciences, Krakow PL-31342, Poland; 3Department of Neurology, Medical University of Bialystok, Bialystok PL-15276, Poland; 4Department of Neurosurgery, Medical University of Bialystok, Bialystok PL-15276, Poland; 5Department of Pathology, Medical University of Bialystok, Bialystok PL-15269, PolandCorrespondence: Robert BuckiDepartment of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2C, Bialystok 15-222, PolandTel +48 85 784 5483Email buckirobert@gmail.comBackground: The tissue-mechanics environment plays a crucial role in human brain physiological development and the pathogenesis of different diseases, especially cancer. Assessment of alterations in brain mechanical  properties during cancer progression might provide important information about possible tissue abnormalities with clinical relevance.Methods: With atomic force microscopy (AFM), the stiffness of freshly removed human brain tumor tissue was determined on various regions of the sample and compared to the stiffness of healthy human brain tissue that was removed during neurosurgery to gain access to tumor mass. An advantage of indentation measurement using AFM is the small volume of tissue required and high resolution at the single-cell level.Results: Our results showed great heterogeneity of stiffness within metastatic cancer or primary high-grade gliomas compared to healthy tissue. That effect was not clearly visible in lower-grade tumors like meningioma.Conclusion: Collected data indicate that AFM might serve as a diagnostic tool in the assessment of human brain tissue stiffness in the process of recognizing tumors.Keywords: AFM, brain tumors, human tissue rheology, mechanomarkers, glioblastomaCieśluk MPogoda KDeptuła PWerel PKułakowska AKochanowicz JMariak ZŁysoń TReszeć JBucki RDove Medical Pressarticleafmbrain tumorshuman tissue rheologymechanomarkersglioblastomaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 7509-7521 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	afm brain tumors human tissue rheology mechanomarkers glioblastoma Medicine (General) R5-920
spellingShingle	afm brain tumors human tissue rheology mechanomarkers glioblastoma Medicine (General) R5-920 Cieśluk M Pogoda K Deptuła P Werel P Kułakowska A Kochanowicz J Mariak Z Łysoń T Reszeć J Bucki R Nanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors
description	Mateusz Cieśluk,1 Katarzyna Pogoda,2 Piotr Deptuła,1 Paulina Werel,3 Alina Kułakowska,3 Jan Kochanowicz,3 Zenon Mariak,4 Tomasz Łysoń,4 Joanna Reszeć,5 Robert Bucki1 1Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Bialystok PL-15222, Poland; 2Institute of Nuclear Physics, Polish Academy of Sciences, Krakow PL-31342, Poland; 3Department of Neurology, Medical University of Bialystok, Bialystok PL-15276, Poland; 4Department of Neurosurgery, Medical University of Bialystok, Bialystok PL-15276, Poland; 5Department of Pathology, Medical University of Bialystok, Bialystok PL-15269, PolandCorrespondence: Robert BuckiDepartment of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2C, Bialystok 15-222, PolandTel +48 85 784 5483Email buckirobert@gmail.comBackground: The tissue-mechanics environment plays a crucial role in human brain physiological development and the pathogenesis of different diseases, especially cancer. Assessment of alterations in brain mechanical  properties during cancer progression might provide important information about possible tissue abnormalities with clinical relevance.Methods: With atomic force microscopy (AFM), the stiffness of freshly removed human brain tumor tissue was determined on various regions of the sample and compared to the stiffness of healthy human brain tissue that was removed during neurosurgery to gain access to tumor mass. An advantage of indentation measurement using AFM is the small volume of tissue required and high resolution at the single-cell level.Results: Our results showed great heterogeneity of stiffness within metastatic cancer or primary high-grade gliomas compared to healthy tissue. That effect was not clearly visible in lower-grade tumors like meningioma.Conclusion: Collected data indicate that AFM might serve as a diagnostic tool in the assessment of human brain tissue stiffness in the process of recognizing tumors.Keywords: AFM, brain tumors, human tissue rheology, mechanomarkers, glioblastoma
format	article
author	Cieśluk M Pogoda K Deptuła P Werel P Kułakowska A Kochanowicz J Mariak Z Łysoń T Reszeć J Bucki R
author_facet	Cieśluk M Pogoda K Deptuła P Werel P Kułakowska A Kochanowicz J Mariak Z Łysoń T Reszeć J Bucki R
author_sort	Cieśluk M
title	Nanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors
title_short	Nanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors
title_full	Nanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors
title_fullStr	Nanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors
title_full_unstemmed	Nanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors
title_sort	nanomechanics and histopathology as diagnostic tools to characterize freshly removed human brain tumors
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/a27fb3e5b71c42ce84ee6df823fd524b
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Nanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors

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