A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion

Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chi...

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Autores principales:	Gaurav Pendharkar, Yen-Ta Lu, Chia-Ming Chang, Meng-Ping Lu, Chung-Huan Lu, Chih-Chen Chen, Cheng-Hsien Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hydrodynamic trapping cell pairing dielectrophoresis cell fusion Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a28030de020f474ebe8c3e652fa35f1a
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spelling	oai:doaj.org-article:a28030de020f474ebe8c3e652fa35f1a2021-11-25T17:08:06ZA Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion10.3390/cells101128552073-4409https://doaj.org/article/a28030de020f474ebe8c3e652fa35f1a2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2855https://doaj.org/toc/2073-4409Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chip (MFC) consisting of a thin through-hole membrane sandwiched between a mirrored array of microfluidic channels and saw-tooth shaped titanium electrodes on the glass. We discuss the design and operation of MFC and show its applicability for cell fusion. The proposed device combines passive hydrodynamic phenomenon and gravitational sedimentation, which allows the transportation and trapping of homotypic and heterotypic cells in large numbers with pairing efficiencies of 75~78% and fusion efficiencies of 73%. Additionally, we also report properties of fused cells from cell biology perspectives, including combined fluorescence-labeled intracellular materials from THP1 and A549, mixed cell morphology, and cell viability. The MFC can be tuned for pairing and fusion of cells with a similar protocol for different cell types. The MFC can be easily disconnected from the test setup for further analysis.Gaurav PendharkarYen-Ta LuChia-Ming ChangMeng-Ping LuChung-Huan LuChih-Chen ChenCheng-Hsien LiuMDPI AGarticlehydrodynamic trappingcell pairingdielectrophoresiscell fusionBiology (General)QH301-705.5ENCells, Vol 10, Iss 2855, p 2855 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydrodynamic trapping cell pairing dielectrophoresis cell fusion Biology (General) QH301-705.5
spellingShingle	hydrodynamic trapping cell pairing dielectrophoresis cell fusion Biology (General) QH301-705.5 Gaurav Pendharkar Yen-Ta Lu Chia-Ming Chang Meng-Ping Lu Chung-Huan Lu Chih-Chen Chen Cheng-Hsien Liu A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
description	Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chip (MFC) consisting of a thin through-hole membrane sandwiched between a mirrored array of microfluidic channels and saw-tooth shaped titanium electrodes on the glass. We discuss the design and operation of MFC and show its applicability for cell fusion. The proposed device combines passive hydrodynamic phenomenon and gravitational sedimentation, which allows the transportation and trapping of homotypic and heterotypic cells in large numbers with pairing efficiencies of 75~78% and fusion efficiencies of 73%. Additionally, we also report properties of fused cells from cell biology perspectives, including combined fluorescence-labeled intracellular materials from THP1 and A549, mixed cell morphology, and cell viability. The MFC can be tuned for pairing and fusion of cells with a similar protocol for different cell types. The MFC can be easily disconnected from the test setup for further analysis.
format	article
author	Gaurav Pendharkar Yen-Ta Lu Chia-Ming Chang Meng-Ping Lu Chung-Huan Lu Chih-Chen Chen Cheng-Hsien Liu
author_facet	Gaurav Pendharkar Yen-Ta Lu Chia-Ming Chang Meng-Ping Lu Chung-Huan Lu Chih-Chen Chen Cheng-Hsien Liu
author_sort	Gaurav Pendharkar
title	A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
title_short	A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
title_full	A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
title_fullStr	A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
title_full_unstemmed	A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
title_sort	microfluidic flip-chip combining hydrodynamic trapping and gravitational sedimentation for cell pairing and fusion
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a28030de020f474ebe8c3e652fa35f1a
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A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion

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