Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining

Detection of antigen specificity in a blood sample is limited by low frequencies of individual clones. Here the authors develop an MHC class II combinatorial technique and apply it to immunophenotype human CD4+ T cells of multiple specificities in response to influenza vaccine in single blood sample...

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Autores principales:	Hannes Uchtenhagen, Cliff Rims, Gabriele Blahnik, I-Ting Chow, William W. Kwok, Jane H. Buckner, Eddie A. James
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a2822371a2184c1581f604ee30e21895
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spelling	oai:doaj.org-article:a2822371a2184c1581f604ee30e218952021-12-02T16:57:02ZEfficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining10.1038/ncomms126142041-1723https://doaj.org/article/a2822371a2184c1581f604ee30e218952016-08-01T00:00:00Zhttps://doi.org/10.1038/ncomms12614https://doaj.org/toc/2041-1723Detection of antigen specificity in a blood sample is limited by low frequencies of individual clones. Here the authors develop an MHC class II combinatorial technique and apply it to immunophenotype human CD4+ T cells of multiple specificities in response to influenza vaccine in single blood samples.Hannes UchtenhagenCliff RimsGabriele BlahnikI-Ting ChowWilliam W. KwokJane H. BucknerEddie A. JamesNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-12 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Hannes Uchtenhagen Cliff Rims Gabriele Blahnik I-Ting Chow William W. Kwok Jane H. Buckner Eddie A. James Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
description	Detection of antigen specificity in a blood sample is limited by low frequencies of individual clones. Here the authors develop an MHC class II combinatorial technique and apply it to immunophenotype human CD4+ T cells of multiple specificities in response to influenza vaccine in single blood samples.
format	article
author	Hannes Uchtenhagen Cliff Rims Gabriele Blahnik I-Ting Chow William W. Kwok Jane H. Buckner Eddie A. James
author_facet	Hannes Uchtenhagen Cliff Rims Gabriele Blahnik I-Ting Chow William W. Kwok Jane H. Buckner Eddie A. James
author_sort	Hannes Uchtenhagen
title	Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
title_short	Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
title_full	Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
title_fullStr	Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
title_full_unstemmed	Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
title_sort	efficient ex vivo analysis of cd4+ t-cell responses using combinatorial hla class ii tetramer staining
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/a2822371a2184c1581f604ee30e21895
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Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining

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