Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma

Abstract ATP-binding cassette (ABC) and solute carrier (SLC) transporters translocate diverse substances across cellular membranes and their deregulation may cause drug resistance of cancers. This study investigated significance of protein expression and cellular localization of the previously sugge...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lenka Cervenkova, Ondrej Vycital, Jan Bruha, Jachym Rosendorf, Richard Palek, Vaclav Liska, Ondrej Daum, Beatrice Mohelnikova-Duchonova, Pavel Soucek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a28799568d2c4b808e2376b6dbec6361
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a28799568d2c4b808e2376b6dbec6361
record_format	dspace
spelling	oai:doaj.org-article:a28799568d2c4b808e2376b6dbec63612021-12-02T13:35:12ZProtein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma10.1038/s41598-019-56059-w2045-2322https://doaj.org/article/a28799568d2c4b808e2376b6dbec63612019-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-56059-whttps://doaj.org/toc/2045-2322Abstract ATP-binding cassette (ABC) and solute carrier (SLC) transporters translocate diverse substances across cellular membranes and their deregulation may cause drug resistance of cancers. This study investigated significance of protein expression and cellular localization of the previously suggested putative prognostic markers ABCC2 and SLC22A3 in pancreatic cancer patients. Protein localization and brush border staining intensity of ABCC2 and SLC22A3 was assessed in tumor tissue blocks of 65 pancreatic cancer patients and associated with clinical data and survival of patients with regard to therapy. Negative SLC22A3 brush border staining in pancreatic tumors significantly increased the risk of both disease progression and patient´s death in univariate analyses. Multivariate analyses confirmed the association of SLC22A3 expression with progression-free survival of patients. A subgroup analysis of patients treated with regimens based on nucleoside analogs suggested that patients with negative brush border staining or apical localization of SLC22A3 in tumor cells have worse overall survival. The combination of positive ABCC2 and negative SLC22A3 brush border staining predicted worst overall survival and patients with positive brush border staining of both proteins had best overall and progression-free survival. The present study shows for the first time that the protein presence and to some extent also localization of SLC22A3 significantly associate with prognosis of pancreatic cancer in both unstratified and chemotherapy-treated patients. The combination of ABCC2 and SLC22A3 brush border staining also needs further attention in this regard.Lenka CervenkovaOndrej VycitalJan BruhaJachym RosendorfRichard PalekVaclav LiskaOndrej DaumBeatrice Mohelnikova-DuchonovaPavel SoucekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lenka Cervenkova Ondrej Vycital Jan Bruha Jachym Rosendorf Richard Palek Vaclav Liska Ondrej Daum Beatrice Mohelnikova-Duchonova Pavel Soucek Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma
description	Abstract ATP-binding cassette (ABC) and solute carrier (SLC) transporters translocate diverse substances across cellular membranes and their deregulation may cause drug resistance of cancers. This study investigated significance of protein expression and cellular localization of the previously suggested putative prognostic markers ABCC2 and SLC22A3 in pancreatic cancer patients. Protein localization and brush border staining intensity of ABCC2 and SLC22A3 was assessed in tumor tissue blocks of 65 pancreatic cancer patients and associated with clinical data and survival of patients with regard to therapy. Negative SLC22A3 brush border staining in pancreatic tumors significantly increased the risk of both disease progression and patient´s death in univariate analyses. Multivariate analyses confirmed the association of SLC22A3 expression with progression-free survival of patients. A subgroup analysis of patients treated with regimens based on nucleoside analogs suggested that patients with negative brush border staining or apical localization of SLC22A3 in tumor cells have worse overall survival. The combination of positive ABCC2 and negative SLC22A3 brush border staining predicted worst overall survival and patients with positive brush border staining of both proteins had best overall and progression-free survival. The present study shows for the first time that the protein presence and to some extent also localization of SLC22A3 significantly associate with prognosis of pancreatic cancer in both unstratified and chemotherapy-treated patients. The combination of ABCC2 and SLC22A3 brush border staining also needs further attention in this regard.
format	article
author	Lenka Cervenkova Ondrej Vycital Jan Bruha Jachym Rosendorf Richard Palek Vaclav Liska Ondrej Daum Beatrice Mohelnikova-Duchonova Pavel Soucek
author_facet	Lenka Cervenkova Ondrej Vycital Jan Bruha Jachym Rosendorf Richard Palek Vaclav Liska Ondrej Daum Beatrice Mohelnikova-Duchonova Pavel Soucek
author_sort	Lenka Cervenkova
title	Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma
title_short	Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma
title_full	Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma
title_fullStr	Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma
title_full_unstemmed	Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma
title_sort	protein expression of abcc2 and slc22a3 associates with prognosis of pancreatic adenocarcinoma
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/a28799568d2c4b808e2376b6dbec6361
work_keys_str_mv	AT lenkacervenkova proteinexpressionofabcc2andslc22a3associateswithprognosisofpancreaticadenocarcinoma AT ondrejvycital proteinexpressionofabcc2andslc22a3associateswithprognosisofpancreaticadenocarcinoma AT janbruha proteinexpressionofabcc2andslc22a3associateswithprognosisofpancreaticadenocarcinoma AT jachymrosendorf proteinexpressionofabcc2andslc22a3associateswithprognosisofpancreaticadenocarcinoma AT richardpalek proteinexpressionofabcc2andslc22a3associateswithprognosisofpancreaticadenocarcinoma AT vaclavliska proteinexpressionofabcc2andslc22a3associateswithprognosisofpancreaticadenocarcinoma AT ondrejdaum proteinexpressionofabcc2andslc22a3associateswithprognosisofpancreaticadenocarcinoma AT beatricemohelnikovaduchonova proteinexpressionofabcc2andslc22a3associateswithprognosisofpancreaticadenocarcinoma AT pavelsoucek proteinexpressionofabcc2andslc22a3associateswithprognosisofpancreaticadenocarcinoma
_version_	1718392691079774208

Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma

Ejemplares similares