An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in <i>Nicotiana benthamiana</i>

The tripartite genome of the negative-stranded RNA virus <i>Tomato spotted wilt orthotospovirus</i> (TSWV) is assembled, together with two viral proteins, the nucleocapsid protein and the RNA-dependent RNA polymerase, into infectious ribonucleoprotein complexes (RNPs). These two viral pr...

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Autores principales:	Tieme A. Helderman, Laurens Deurhof, André Bertran, Sjef Boeren, Like Fokkens, Richard Kormelink, Matthieu H. A. J. Joosten, Marcel Prins, Harrold A. van den Burg
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	eukaryotic translation elongation factor 1A (eEF1A) host factor nanoLC-MS/MS SDS-PAGE-based proteomics pro-viral factor ribonucleoproteins (RNP) Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a28938f2ff464873b27ef06684ce99df
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spelling	oai:doaj.org-article:a28938f2ff464873b27ef06684ce99df2021-11-25T19:13:12ZAn Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in <i>Nicotiana benthamiana</i>10.3390/v131121901999-4915https://doaj.org/article/a28938f2ff464873b27ef06684ce99df2021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2190https://doaj.org/toc/1999-4915The tripartite genome of the negative-stranded RNA virus <i>Tomato spotted wilt orthotospovirus</i> (TSWV) is assembled, together with two viral proteins, the nucleocapsid protein and the RNA-dependent RNA polymerase, into infectious ribonucleoprotein complexes (RNPs). These two viral proteins are, together, essential for viral replication and transcription, yet our knowledge on the host factors supporting these two processes remains limited. To fill this knowledge gap, the protein composition of viral RNPs collected from TSWV-infected <i>Nicotiana benthamiana</i> plants, and of those collected from a reconstituted TSWV replicon system in the yeast <i>Saccharomyces cerevisiae</i>, was analysed. RNPs obtained from infected plant material were enriched for plant proteins implicated in (<i>i</i>) sugar and phosphate transport and (<i>ii</i>) responses to cellular stress. In contrast, the yeast-derived viral RNPs primarily contained proteins implicated in RNA processing and ribosome biogenesis. The latter suggests that, in yeast, the translational machinery is recruited to these viral RNPs. To examine whether one of these cellular proteins is important for a TSWV infection, the corresponding <i>N. benthamiana</i> genes were targeted for virus-induced gene silencing, and these plants were subsequently challenged with TSWV. This approach revealed four host factors that are important for systemic spread of TSWV and disease symptom development.Tieme A. HeldermanLaurens DeurhofAndré BertranSjef BoerenLike FokkensRichard KormelinkMatthieu H. A. J. JoostenMarcel PrinsHarrold A. van den BurgMDPI AGarticleeukaryotic translation elongation factor 1A (eEF1A)host factornanoLC-MS/MSSDS-PAGE-based proteomicspro-viral factorribonucleoproteins (RNP)MicrobiologyQR1-502ENViruses, Vol 13, Iss 2190, p 2190 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	eukaryotic translation elongation factor 1A (eEF1A) host factor nanoLC-MS/MS SDS-PAGE-based proteomics pro-viral factor ribonucleoproteins (RNP) Microbiology QR1-502
spellingShingle	eukaryotic translation elongation factor 1A (eEF1A) host factor nanoLC-MS/MS SDS-PAGE-based proteomics pro-viral factor ribonucleoproteins (RNP) Microbiology QR1-502 Tieme A. Helderman Laurens Deurhof André Bertran Sjef Boeren Like Fokkens Richard Kormelink Matthieu H. A. J. Joosten Marcel Prins Harrold A. van den Burg An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in <i>Nicotiana benthamiana</i>
description	The tripartite genome of the negative-stranded RNA virus <i>Tomato spotted wilt orthotospovirus</i> (TSWV) is assembled, together with two viral proteins, the nucleocapsid protein and the RNA-dependent RNA polymerase, into infectious ribonucleoprotein complexes (RNPs). These two viral proteins are, together, essential for viral replication and transcription, yet our knowledge on the host factors supporting these two processes remains limited. To fill this knowledge gap, the protein composition of viral RNPs collected from TSWV-infected <i>Nicotiana benthamiana</i> plants, and of those collected from a reconstituted TSWV replicon system in the yeast <i>Saccharomyces cerevisiae</i>, was analysed. RNPs obtained from infected plant material were enriched for plant proteins implicated in (<i>i</i>) sugar and phosphate transport and (<i>ii</i>) responses to cellular stress. In contrast, the yeast-derived viral RNPs primarily contained proteins implicated in RNA processing and ribosome biogenesis. The latter suggests that, in yeast, the translational machinery is recruited to these viral RNPs. To examine whether one of these cellular proteins is important for a TSWV infection, the corresponding <i>N. benthamiana</i> genes were targeted for virus-induced gene silencing, and these plants were subsequently challenged with TSWV. This approach revealed four host factors that are important for systemic spread of TSWV and disease symptom development.
format	article
author	Tieme A. Helderman Laurens Deurhof André Bertran Sjef Boeren Like Fokkens Richard Kormelink Matthieu H. A. J. Joosten Marcel Prins Harrold A. van den Burg
author_facet	Tieme A. Helderman Laurens Deurhof André Bertran Sjef Boeren Like Fokkens Richard Kormelink Matthieu H. A. J. Joosten Marcel Prins Harrold A. van den Burg
author_sort	Tieme A. Helderman
title	An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in <i>Nicotiana benthamiana</i>
title_short	An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in <i>Nicotiana benthamiana</i>
title_full	An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in <i>Nicotiana benthamiana</i>
title_fullStr	An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in <i>Nicotiana benthamiana</i>
title_full_unstemmed	An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in <i>Nicotiana benthamiana</i>
title_sort	isoform of the eukaryotic translation elongation factor 1a (eef1a) acts as a pro-viral factor required for tomato spotted wilt virus disease in <i>nicotiana benthamiana</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a28938f2ff464873b27ef06684ce99df
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An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in <i>Nicotiana benthamiana</i>

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