Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung

Abstract. Afifah D, Lesmana I, Poerwanto SH, Joko T, Mahardhika IWS, Daryono BS. 2020. Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung. Biodiversitas 21: 1483-1487. The Gama Ayam Research Team has implemented marker-as...

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Autores principales:	Desiana Afifah, Indra Lesmana, Soenarwan Hery Poerwanto, Tri Joko, swarautama mahardhika, Budi Setiadi Daryono
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	ai, kamper, mx gene, resistance, snps Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a289fcf4f3da4c9091dec70123744dae
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spelling	oai:doaj.org-article:a289fcf4f3da4c9091dec70123744dae2021-11-22T00:34:40ZExpression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung1412-033X2085-472210.13057/biodiv/d210428https://doaj.org/article/a289fcf4f3da4c9091dec70123744dae2020-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5045https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Afifah D, Lesmana I, Poerwanto SH, Joko T, Mahardhika IWS, Daryono BS. 2020. Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung. Biodiversitas 21: 1483-1487. The Gama Ayam Research Team has implemented marker-assisted selection (MAS) in selective breeding to provide a faster, more accurate, and more reliable selection of chicken. Mx gene expression has a vital role in chicken disease resistance. This research aimed to investigate the expression of Mx exon-13 single nucleotide polymorphisms (SNPs) in the population of female Layer Lohmann Brown-Classic, male Pelung, and its progenies Kampong-Laying Type (Kamper) chicken. The G1892A mutation in Mx exon-13 resulted in a change in the amino acid 631 of Mx. The substitution of serine to asparagine favored the ability of chickens to acquire immunity against viral diseases, including avian influenza. Asparagine (A allele) at position 631 is specific to Mx+ (resistant), whereas serine (G allele) is specific to Mx? (susceptible). DNA was amplified using the forward primer 5?-GCACTGTCACCTCTTAATAGA-3? and the reverse primer 5?-GTATTGGTAGGCTTTGTTGA-3? and then sequenced using the Sanger sequencing method. Four SNPs were obtained through Mx sequence alignment. They consisted of four substitutions (A20734T, C20737T, A20766G, and A20893G) with one haplotype. Mx exon-13 SNPs were detected in Pelung and Kamper. Therefore, Kamper chicken inherited the disease resistance gene of Pelung and could be a strong candidate for parental generation in the further selective breeding program.Desiana AfifahIndra LesmanaSoenarwan Hery PoerwantoTri Jokoswarautama mahardhikaBudi Setiadi DaryonoMBI & UNS Soloarticleai, kamper, mx gene, resistance, snpsBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	ai, kamper, mx gene, resistance, snps Biology (General) QH301-705.5
spellingShingle	ai, kamper, mx gene, resistance, snps Biology (General) QH301-705.5 Desiana Afifah Indra Lesmana Soenarwan Hery Poerwanto Tri Joko swarautama mahardhika Budi Setiadi Daryono Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung
description	Abstract. Afifah D, Lesmana I, Poerwanto SH, Joko T, Mahardhika IWS, Daryono BS. 2020. Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung. Biodiversitas 21: 1483-1487. The Gama Ayam Research Team has implemented marker-assisted selection (MAS) in selective breeding to provide a faster, more accurate, and more reliable selection of chicken. Mx gene expression has a vital role in chicken disease resistance. This research aimed to investigate the expression of Mx exon-13 single nucleotide polymorphisms (SNPs) in the population of female Layer Lohmann Brown-Classic, male Pelung, and its progenies Kampong-Laying Type (Kamper) chicken. The G1892A mutation in Mx exon-13 resulted in a change in the amino acid 631 of Mx. The substitution of serine to asparagine favored the ability of chickens to acquire immunity against viral diseases, including avian influenza. Asparagine (A allele) at position 631 is specific to Mx+ (resistant), whereas serine (G allele) is specific to Mx? (susceptible). DNA was amplified using the forward primer 5?-GCACTGTCACCTCTTAATAGA-3? and the reverse primer 5?-GTATTGGTAGGCTTTGTTGA-3? and then sequenced using the Sanger sequencing method. Four SNPs were obtained through Mx sequence alignment. They consisted of four substitutions (A20734T, C20737T, A20766G, and A20893G) with one haplotype. Mx exon-13 SNPs were detected in Pelung and Kamper. Therefore, Kamper chicken inherited the disease resistance gene of Pelung and could be a strong candidate for parental generation in the further selective breeding program.
format	article
author	Desiana Afifah Indra Lesmana Soenarwan Hery Poerwanto Tri Joko swarautama mahardhika Budi Setiadi Daryono
author_facet	Desiana Afifah Indra Lesmana Soenarwan Hery Poerwanto Tri Joko swarautama mahardhika Budi Setiadi Daryono
author_sort	Desiana Afifah
title	Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung
title_short	Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung
title_full	Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung
title_fullStr	Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung
title_full_unstemmed	Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung
title_sort	expression of mx exon-13 snps in kampong-laying type (kamper) chicken crossbreeds of female lohmann brown-classic and male pelung
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/a289fcf4f3da4c9091dec70123744dae
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Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung

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