Identifikasi Thrips alliorum (Priesner), Thrips hawaiiensis (Morgan), dan Thrips parvispinus (Karny) berdasarkan variasi DNA COI mitokondria

Thrips is the second largest genus on the order of Thysanoptera, most of them are polyphagous and some species are serious pests on vegetables. The damages caused by thrips can reach 30–50% yield loss. Thrips alliorum (Priesner), T. hawaiiensis (Morgan), and T. parvispinus (Karny) are widely reporte...

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Autores principales: Nia Kurniawaty, Purnama Hidayat, Aunu Rauf
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Publicado: The Entomological Society of Indonesia 2017
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spelling oai:doaj.org-article:a2910852f92d4b0d83bf80c09552fa352021-12-02T07:09:04ZIdentifikasi Thrips alliorum (Priesner), Thrips hawaiiensis (Morgan), dan Thrips parvispinus (Karny) berdasarkan variasi DNA COI mitokondria10.5994/jei.14.1.201829-77222089-0257https://doaj.org/article/a2910852f92d4b0d83bf80c09552fa352017-03-01T00:00:00Zhttp://jurnal.pei-pusat.org/index.php/jei/article/view/157https://doaj.org/toc/1829-7722https://doaj.org/toc/2089-0257Thrips is the second largest genus on the order of Thysanoptera, most of them are polyphagous and some species are serious pests on vegetables. The damages caused by thrips can reach 30–50% yield loss. Thrips alliorum (Priesner), T. hawaiiensis (Morgan), and T. parvispinus (Karny) are widely reported as pests on crops, especially on horticulture. This study aimed to identify three trhips species: Thrips alliorum, T. hawaiiensis, and T. parvispinus based on the DNA sequences of mtCOI gene fragment. Thrips samples were collected from Bandung, Bogor, Cianjur, and Kuningan districts. Three steps, they were sample collection and DNA total extraction, amplification by using PCR and DNA sequence analysis. The PCR succesfully amplified DNA of mtCOI gene fragments of T. alliorum, T. hawaiiensis, and T. parvispinus at 678, 690, and 668 bp respectively. The mtCOI DNA sequences were dominated by A and T bases with the nucleotide variation value of 25.18%. Identification of the three thrips species: T. alliorum, T. hawaiiensis, and T. parvispinus based on molecular characters using mtCOI DNA sequences confirmed the identification result based on the morphological caharacters.Nia KurniawatyPurnama HidayatAunu RaufThe Entomological Society of IndonesiaarticleCOI; mitochondrial; pest; sequence; taxonomyZoologyQL1-991ENIDJurnal Entomologi Indonesia, Vol 14, Iss 1, Pp 20-28 (2017)
institution DOAJ
collection DOAJ
language EN
ID
topic COI; mitochondrial; pest; sequence; taxonomy
Zoology
QL1-991
spellingShingle COI; mitochondrial; pest; sequence; taxonomy
Zoology
QL1-991
Nia Kurniawaty
Purnama Hidayat
Aunu Rauf
Identifikasi Thrips alliorum (Priesner), Thrips hawaiiensis (Morgan), dan Thrips parvispinus (Karny) berdasarkan variasi DNA COI mitokondria
description Thrips is the second largest genus on the order of Thysanoptera, most of them are polyphagous and some species are serious pests on vegetables. The damages caused by thrips can reach 30–50% yield loss. Thrips alliorum (Priesner), T. hawaiiensis (Morgan), and T. parvispinus (Karny) are widely reported as pests on crops, especially on horticulture. This study aimed to identify three trhips species: Thrips alliorum, T. hawaiiensis, and T. parvispinus based on the DNA sequences of mtCOI gene fragment. Thrips samples were collected from Bandung, Bogor, Cianjur, and Kuningan districts. Three steps, they were sample collection and DNA total extraction, amplification by using PCR and DNA sequence analysis. The PCR succesfully amplified DNA of mtCOI gene fragments of T. alliorum, T. hawaiiensis, and T. parvispinus at 678, 690, and 668 bp respectively. The mtCOI DNA sequences were dominated by A and T bases with the nucleotide variation value of 25.18%. Identification of the three thrips species: T. alliorum, T. hawaiiensis, and T. parvispinus based on molecular characters using mtCOI DNA sequences confirmed the identification result based on the morphological caharacters.
format article
author Nia Kurniawaty
Purnama Hidayat
Aunu Rauf
author_facet Nia Kurniawaty
Purnama Hidayat
Aunu Rauf
author_sort Nia Kurniawaty
title Identifikasi Thrips alliorum (Priesner), Thrips hawaiiensis (Morgan), dan Thrips parvispinus (Karny) berdasarkan variasi DNA COI mitokondria
title_short Identifikasi Thrips alliorum (Priesner), Thrips hawaiiensis (Morgan), dan Thrips parvispinus (Karny) berdasarkan variasi DNA COI mitokondria
title_full Identifikasi Thrips alliorum (Priesner), Thrips hawaiiensis (Morgan), dan Thrips parvispinus (Karny) berdasarkan variasi DNA COI mitokondria
title_fullStr Identifikasi Thrips alliorum (Priesner), Thrips hawaiiensis (Morgan), dan Thrips parvispinus (Karny) berdasarkan variasi DNA COI mitokondria
title_full_unstemmed Identifikasi Thrips alliorum (Priesner), Thrips hawaiiensis (Morgan), dan Thrips parvispinus (Karny) berdasarkan variasi DNA COI mitokondria
title_sort identifikasi thrips alliorum (priesner), thrips hawaiiensis (morgan), dan thrips parvispinus (karny) berdasarkan variasi dna coi mitokondria
publisher The Entomological Society of Indonesia
publishDate 2017
url https://doaj.org/article/a2910852f92d4b0d83bf80c09552fa35
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