Analysis of the possible cytogenetic mechanism for overcoming hybrid lethality in an interspecific cross between Nicotiana suaveolens and Nicotiana tabacum

Abstract Hybrid lethality is a type of reproductive isolation in which hybrids die before maturation, due to the interaction between the two causative genes derived from each of the hybrid parents. The interspecific hybrid of Nicotiana suaveolens × Nicotiana tabacum is a model plant used in studies...

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Autores principales:	Kouki Nakata, Hiroki Nagashima, Natsuki Inaba, Haruka Yamashita, Yoshihito Shinozaki, Motoki Kanekatsu, Wataru Marubashi, Tetsuya Yamada
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a29513c358f54e3cac11c7346f132e7e
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spelling	oai:doaj.org-article:a29513c358f54e3cac11c7346f132e7e2021-12-02T14:22:43ZAnalysis of the possible cytogenetic mechanism for overcoming hybrid lethality in an interspecific cross between Nicotiana suaveolens and Nicotiana tabacum10.1038/s41598-021-87242-72045-2322https://doaj.org/article/a29513c358f54e3cac11c7346f132e7e2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87242-7https://doaj.org/toc/2045-2322Abstract Hybrid lethality is a type of reproductive isolation in which hybrids die before maturation, due to the interaction between the two causative genes derived from each of the hybrid parents. The interspecific hybrid of Nicotiana suaveolens × Nicotiana tabacum is a model plant used in studies on hybrid lethality. While most of the progeny produced from such a cross die, some individuals grow normally and mature. Separately, a technique for producing mature hybrids by artificial culture has been developed. However, the mechanism by which hybrids overcome lethality, either spontaneously or by artificial culture, remains unclear. In the present study, we found that some hybrids that overcome lethality, either spontaneously or by artificial culture, lack the distal part of the Q chromosome, a region that includes the gene responsible for lethality. Quantitative polymerase chain reaction results suggested that the distal deletion of the Q chromosome, detected in some hybrid seedlings that overcome lethality, is caused by reciprocal translocations between homoeologous chromosomes. The results showed that chromosomal instability during meiosis in amphidiploid N. tabacum as well as during artificial culturing of hybrid seedlings is involved in overcoming hybrid lethality in interspecific crosses of the genus Nicotiana.Kouki NakataHiroki NagashimaNatsuki InabaHaruka YamashitaYoshihito ShinozakiMotoki KanekatsuWataru MarubashiTetsuya YamadaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kouki Nakata Hiroki Nagashima Natsuki Inaba Haruka Yamashita Yoshihito Shinozaki Motoki Kanekatsu Wataru Marubashi Tetsuya Yamada Analysis of the possible cytogenetic mechanism for overcoming hybrid lethality in an interspecific cross between Nicotiana suaveolens and Nicotiana tabacum
description	Abstract Hybrid lethality is a type of reproductive isolation in which hybrids die before maturation, due to the interaction between the two causative genes derived from each of the hybrid parents. The interspecific hybrid of Nicotiana suaveolens × Nicotiana tabacum is a model plant used in studies on hybrid lethality. While most of the progeny produced from such a cross die, some individuals grow normally and mature. Separately, a technique for producing mature hybrids by artificial culture has been developed. However, the mechanism by which hybrids overcome lethality, either spontaneously or by artificial culture, remains unclear. In the present study, we found that some hybrids that overcome lethality, either spontaneously or by artificial culture, lack the distal part of the Q chromosome, a region that includes the gene responsible for lethality. Quantitative polymerase chain reaction results suggested that the distal deletion of the Q chromosome, detected in some hybrid seedlings that overcome lethality, is caused by reciprocal translocations between homoeologous chromosomes. The results showed that chromosomal instability during meiosis in amphidiploid N. tabacum as well as during artificial culturing of hybrid seedlings is involved in overcoming hybrid lethality in interspecific crosses of the genus Nicotiana.
format	article
author	Kouki Nakata Hiroki Nagashima Natsuki Inaba Haruka Yamashita Yoshihito Shinozaki Motoki Kanekatsu Wataru Marubashi Tetsuya Yamada
author_facet	Kouki Nakata Hiroki Nagashima Natsuki Inaba Haruka Yamashita Yoshihito Shinozaki Motoki Kanekatsu Wataru Marubashi Tetsuya Yamada
author_sort	Kouki Nakata
title	Analysis of the possible cytogenetic mechanism for overcoming hybrid lethality in an interspecific cross between Nicotiana suaveolens and Nicotiana tabacum
title_short	Analysis of the possible cytogenetic mechanism for overcoming hybrid lethality in an interspecific cross between Nicotiana suaveolens and Nicotiana tabacum
title_full	Analysis of the possible cytogenetic mechanism for overcoming hybrid lethality in an interspecific cross between Nicotiana suaveolens and Nicotiana tabacum
title_fullStr	Analysis of the possible cytogenetic mechanism for overcoming hybrid lethality in an interspecific cross between Nicotiana suaveolens and Nicotiana tabacum
title_full_unstemmed	Analysis of the possible cytogenetic mechanism for overcoming hybrid lethality in an interspecific cross between Nicotiana suaveolens and Nicotiana tabacum
title_sort	analysis of the possible cytogenetic mechanism for overcoming hybrid lethality in an interspecific cross between nicotiana suaveolens and nicotiana tabacum
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a29513c358f54e3cac11c7346f132e7e
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Analysis of the possible cytogenetic mechanism for overcoming hybrid lethality in an interspecific cross between Nicotiana suaveolens and Nicotiana tabacum

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