A single dose of dexamethasone encapsulated in polyethylene glycol-coated polylactic acid nanoparticles attenuates cisplatin-induced hearing loss following round window membrane administration

Changling Sun,1,3,* Xueling Wang,1,* Zhaozhu Zheng,2 Dongye Chen,1 Xiaoqin Wang,2 Fuxin Shi,1 Dehong Yu,1 Hao Wu11Department of Otolaryngology–Head and Neck Surgery, Xinhua Hospital, Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Translational M...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sun CL, Wang XL, Zheng ZZ, Chen DY, Wang XQ, Shi F, Yu DH, Wu H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a29841bf71244739999ecbc4fd68edc9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a29841bf71244739999ecbc4fd68edc9
record_format	dspace
spelling	oai:doaj.org-article:a29841bf71244739999ecbc4fd68edc92021-12-02T05:03:08ZA single dose of dexamethasone encapsulated in polyethylene glycol-coated polylactic acid nanoparticles attenuates cisplatin-induced hearing loss following round window membrane administration1178-2013https://doaj.org/article/a29841bf71244739999ecbc4fd68edc92015-05-01T00:00:00Zhttp://www.dovepress.com/a-single-dose-of-dexamethasone-encapsulated-in-polyethylene-glycol-coa-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Changling Sun,1,3,* Xueling Wang,1,* Zhaozhu Zheng,2 Dongye Chen,1 Xiaoqin Wang,2 Fuxin Shi,1 Dehong Yu,1 Hao Wu11Department of Otolaryngology–Head and Neck Surgery, Xinhua Hospital, Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, 2National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, 3Department of Otolaryngology–Head and Neck Surgery, Affiliated Hospital of Jiangnan University, The Fourth People’s Hospital of Wuxi City, Wuxi, People’s Republic of China*These authors have contributed equally to this workAbstract: This study aimed to investigate the sustained drug release properties and hearing protection effect of polyethylene glycol-coated polylactic acid (PEG-PLA) stealth nanoparticles loaded with dexamethasone (DEX). DEX was fabricated into PEG-PLA nanoparticles using an emulsion and evaporation technique, as previously reported. The DEX-loaded PEG-PLA nanoparticles (DEX-NPs) had a hydrodynamic diameter of 130±4.78 nm, and a zeta potential of -26.13±3.28 mV. The in vitro release of DEX from DEX-NPs lasted 24 days in phosphate buffered saline (pH 7.4), 5 days in artificial perilymph (pH 7.4), and 1 day in rat plasma. Coumarin 6-labeled NPs placed onto the round window membrane (RWM) of guinea pigs penetrated RWM quickly and accumulated to the organs of Corti, stria vascularis, and spiral ganglion cells after 1 hour of administration. The DEX-NPs locally applied onto the RWM of guinea pigs by a single-dose administration continuously released DEX in 48 hours, which was significantly longer than the free DEX that was cleared out within 12 hours after administration at the same dose. Further functional studies showed that locally administrated single-dose DEX-NPs effectively preserved outer hair cells in guinea pigs after cisplatin insult and thus significantly attenuated hearing loss at 4 kHz and 8 kHz frequencies when compared to the control of free DEX formulation. Histological analyses indicated that the administration of DEX-NPs did not induce local inflammatory responses. Therefore, prolonged delivery of DEX by PEG-PLA nanoparticles through local RWM diffusion (administration) significantly protected the hair cells and auditory function in guinea pigs from cisplatin toxicity, as determined at both histological and functional levels, suggesting the potential therapeutic benefits in clinical applications.Keywords: stealth nanoparticles, dexamethasone, single-dose administration, cisplatin-induced hearing lossSun CLWang XLZheng ZZChen DYWang XQShi FYu DHWu HDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 3567-3579 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Sun CL Wang XL Zheng ZZ Chen DY Wang XQ Shi F Yu DH Wu H A single dose of dexamethasone encapsulated in polyethylene glycol-coated polylactic acid nanoparticles attenuates cisplatin-induced hearing loss following round window membrane administration
description	Changling Sun,1,3,* Xueling Wang,1,* Zhaozhu Zheng,2 Dongye Chen,1 Xiaoqin Wang,2 Fuxin Shi,1 Dehong Yu,1 Hao Wu11Department of Otolaryngology–Head and Neck Surgery, Xinhua Hospital, Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, 2National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, 3Department of Otolaryngology–Head and Neck Surgery, Affiliated Hospital of Jiangnan University, The Fourth People’s Hospital of Wuxi City, Wuxi, People’s Republic of China*These authors have contributed equally to this workAbstract: This study aimed to investigate the sustained drug release properties and hearing protection effect of polyethylene glycol-coated polylactic acid (PEG-PLA) stealth nanoparticles loaded with dexamethasone (DEX). DEX was fabricated into PEG-PLA nanoparticles using an emulsion and evaporation technique, as previously reported. The DEX-loaded PEG-PLA nanoparticles (DEX-NPs) had a hydrodynamic diameter of 130±4.78 nm, and a zeta potential of -26.13±3.28 mV. The in vitro release of DEX from DEX-NPs lasted 24 days in phosphate buffered saline (pH 7.4), 5 days in artificial perilymph (pH 7.4), and 1 day in rat plasma. Coumarin 6-labeled NPs placed onto the round window membrane (RWM) of guinea pigs penetrated RWM quickly and accumulated to the organs of Corti, stria vascularis, and spiral ganglion cells after 1 hour of administration. The DEX-NPs locally applied onto the RWM of guinea pigs by a single-dose administration continuously released DEX in 48 hours, which was significantly longer than the free DEX that was cleared out within 12 hours after administration at the same dose. Further functional studies showed that locally administrated single-dose DEX-NPs effectively preserved outer hair cells in guinea pigs after cisplatin insult and thus significantly attenuated hearing loss at 4 kHz and 8 kHz frequencies when compared to the control of free DEX formulation. Histological analyses indicated that the administration of DEX-NPs did not induce local inflammatory responses. Therefore, prolonged delivery of DEX by PEG-PLA nanoparticles through local RWM diffusion (administration) significantly protected the hair cells and auditory function in guinea pigs from cisplatin toxicity, as determined at both histological and functional levels, suggesting the potential therapeutic benefits in clinical applications.Keywords: stealth nanoparticles, dexamethasone, single-dose administration, cisplatin-induced hearing loss
format	article
author	Sun CL Wang XL Zheng ZZ Chen DY Wang XQ Shi F Yu DH Wu H
author_facet	Sun CL Wang XL Zheng ZZ Chen DY Wang XQ Shi F Yu DH Wu H
author_sort	Sun CL
title	A single dose of dexamethasone encapsulated in polyethylene glycol-coated polylactic acid nanoparticles attenuates cisplatin-induced hearing loss following round window membrane administration
title_short	A single dose of dexamethasone encapsulated in polyethylene glycol-coated polylactic acid nanoparticles attenuates cisplatin-induced hearing loss following round window membrane administration
title_full	A single dose of dexamethasone encapsulated in polyethylene glycol-coated polylactic acid nanoparticles attenuates cisplatin-induced hearing loss following round window membrane administration
title_fullStr	A single dose of dexamethasone encapsulated in polyethylene glycol-coated polylactic acid nanoparticles attenuates cisplatin-induced hearing loss following round window membrane administration
title_full_unstemmed	A single dose of dexamethasone encapsulated in polyethylene glycol-coated polylactic acid nanoparticles attenuates cisplatin-induced hearing loss following round window membrane administration
title_sort	single dose of dexamethasone encapsulated in polyethylene glycol-coated polylactic acid nanoparticles attenuates cisplatin-induced hearing loss following round window membrane administration
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/a29841bf71244739999ecbc4fd68edc9
work_keys_str_mv	AT suncl asingledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT wangxl asingledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT zhengzz asingledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT chendy asingledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT wangxq asingledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT shif asingledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT yudh asingledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT wuh asingledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT suncl singledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT wangxl singledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT zhengzz singledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT chendy singledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT wangxq singledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT shif singledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT yudh singledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration AT wuh singledoseofdexamethasoneencapsulatedinpolyethyleneglycolcoatedpolylacticacidnanoparticlesattenuatescisplatininducedhearinglossfollowingroundwindowmembraneadministration
_version_	1718400727295983616

A single dose of dexamethasone encapsulated in polyethylene glycol-coated polylactic acid nanoparticles attenuates cisplatin-induced hearing loss following round window membrane administration

Ejemplares similares