Molecular Cloning, Characterization and Expression Analysis of Calreticulin Gene in the Ant Polyrhachis vicina Roger (Hymenoptera: Formicidae)
Calreticulin (CRT) as a ubiquitous and highly conserved calcium-binding protein exists in endoplasmic reticulum (ER), which possesses a variety of biological functions in the regulation of cell calcium homeostasis, molecular chaperoning and innate immunity. In our research, the calreticulin homologo...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Universidade Estadual de Feira de Santana
2014
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/a29fa1eb8bc045858a9c28600b39202b |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:a29fa1eb8bc045858a9c28600b39202b |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:a29fa1eb8bc045858a9c28600b39202b2021-12-02T15:07:12ZMolecular Cloning, Characterization and Expression Analysis of Calreticulin Gene in the Ant Polyrhachis vicina Roger (Hymenoptera: Formicidae)0361-652510.13102/sociobiology.v60i4.355-361https://doaj.org/article/a29fa1eb8bc045858a9c28600b39202b2014-01-01T00:00:00Zhttp://periodicos.uefs.br/ojs/index.php/sociobiology/article/view/212https://doaj.org/toc/0361-6525Calreticulin (CRT) as a ubiquitous and highly conserved calcium-binding protein exists in endoplasmic reticulum (ER), which possesses a variety of biological functions in the regulation of cell calcium homeostasis, molecular chaperoning and innate immunity. In our research, the calreticulin homologous gene (refered as PvCRT) was cloned from the ant <em>Polyrhachis vicina </em>Roger (Hymenoptera: Formicidae), the full-length cDNA of PvCRT is 1584bp base pairs（bp）, contains a 5’-untranslated region of 87bp and a 3’-untranslated region of 246bp. The open reading frame (ORF) of 1251bp encodes 416 amino acids. Using real-time quantitative RT-PCR to study PvCRT mRNA expression levels indicate that this gene was expressed in different developmental stages of castes of <em>P. vicina. </em>The mRNA expression level in both embryos and adults show that PvCRT gene may play some essential roles in the ant’s development.Liping LiuLiang DangGengsi XiFang WangUniversidade Estadual de Feira de SantanaarticleCalreticulin homologous geneMolecular cloningreal-time quantitative RT-PCRPolyrhachis vicinaZoologyQL1-991EcologyQH540-549.5Natural history (General)QH1-278.5ENSociobiology, Vol 60, Iss 4, Pp 355-361 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Calreticulin homologous gene Molecular cloning real-time quantitative RT-PCR Polyrhachis vicina Zoology QL1-991 Ecology QH540-549.5 Natural history (General) QH1-278.5 |
| spellingShingle |
Calreticulin homologous gene Molecular cloning real-time quantitative RT-PCR Polyrhachis vicina Zoology QL1-991 Ecology QH540-549.5 Natural history (General) QH1-278.5 Liping Liu Liang Dang Gengsi Xi Fang Wang Molecular Cloning, Characterization and Expression Analysis of Calreticulin Gene in the Ant Polyrhachis vicina Roger (Hymenoptera: Formicidae) |
| description |
Calreticulin (CRT) as a ubiquitous and highly conserved calcium-binding protein exists in endoplasmic reticulum (ER), which possesses a variety of biological functions in the regulation of cell calcium homeostasis, molecular chaperoning and innate immunity. In our research, the calreticulin homologous gene (refered as PvCRT) was cloned from the ant <em>Polyrhachis vicina </em>Roger (Hymenoptera: Formicidae), the full-length cDNA of PvCRT is 1584bp base pairs（bp）, contains a 5’-untranslated region of 87bp and a 3’-untranslated region of 246bp. The open reading frame (ORF) of 1251bp encodes 416 amino acids. Using real-time quantitative RT-PCR to study PvCRT mRNA expression levels indicate that this gene was expressed in different developmental stages of castes of <em>P. vicina. </em>The mRNA expression level in both embryos and adults show that PvCRT gene may play some essential roles in the ant’s development. |
| format |
article |
| author |
Liping Liu Liang Dang Gengsi Xi Fang Wang |
| author_facet |
Liping Liu Liang Dang Gengsi Xi Fang Wang |
| author_sort |
Liping Liu |
| title |
Molecular Cloning, Characterization and Expression Analysis of Calreticulin Gene in the Ant Polyrhachis vicina Roger (Hymenoptera: Formicidae) |
| title_short |
Molecular Cloning, Characterization and Expression Analysis of Calreticulin Gene in the Ant Polyrhachis vicina Roger (Hymenoptera: Formicidae) |
| title_full |
Molecular Cloning, Characterization and Expression Analysis of Calreticulin Gene in the Ant Polyrhachis vicina Roger (Hymenoptera: Formicidae) |
| title_fullStr |
Molecular Cloning, Characterization and Expression Analysis of Calreticulin Gene in the Ant Polyrhachis vicina Roger (Hymenoptera: Formicidae) |
| title_full_unstemmed |
Molecular Cloning, Characterization and Expression Analysis of Calreticulin Gene in the Ant Polyrhachis vicina Roger (Hymenoptera: Formicidae) |
| title_sort |
molecular cloning, characterization and expression analysis of calreticulin gene in the ant polyrhachis vicina roger (hymenoptera: formicidae) |
| publisher |
Universidade Estadual de Feira de Santana |
| publishDate |
2014 |
| url |
https://doaj.org/article/a29fa1eb8bc045858a9c28600b39202b |
| work_keys_str_mv |
AT lipingliu molecularcloningcharacterizationandexpressionanalysisofcalreticulingeneintheantpolyrhachisvicinarogerhymenopteraformicidae AT liangdang molecularcloningcharacterizationandexpressionanalysisofcalreticulingeneintheantpolyrhachisvicinarogerhymenopteraformicidae AT gengsixi molecularcloningcharacterizationandexpressionanalysisofcalreticulingeneintheantpolyrhachisvicinarogerhymenopteraformicidae AT fangwang molecularcloningcharacterizationandexpressionanalysisofcalreticulingeneintheantpolyrhachisvicinarogerhymenopteraformicidae |
| _version_ |
1718388469226536960 |