Comparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire

Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen associated with a wide variety of infections in humans. The ability of MRSA to infect companion animals has gained increasing attention in the scientific literature. In this study, 334 dogs were screened for MRSA in...

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Autores principales:	Bruno Penna, Marcella B. Silva, André E. R. Soares, Ana T. R. Vasconcelos, Mariana S. Ramundo, Fabienne A. Ferreira, Maria C. Silva-Carvalho, Viviane S. de Sousa, Renata F. Rabello, Paula T. Bandeira, Viviane S. de Souza, Paul J. Planet, Olney Vieira-da-Motta, Ana M. N. Botelho, Agnes M. S. Figueiredo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2a61180361042cc865f912cd9c57bbf
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spelling	oai:doaj.org-article:a2a61180361042cc865f912cd9c57bbf2021-12-02T11:37:22ZComparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire10.1038/s41598-021-83993-52045-2322https://doaj.org/article/a2a61180361042cc865f912cd9c57bbf2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83993-5https://doaj.org/toc/2045-2322Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen associated with a wide variety of infections in humans. The ability of MRSA to infect companion animals has gained increasing attention in the scientific literature. In this study, 334 dogs were screened for MRSA in two cities located in Rio de Janeiro State. The prevalence of MRSA in dogs was 2.7%. Genotyping revealed isolates from sequence types (ST) 1, 5, 30, and 239 either colonizing or infecting dogs. The genome of the canine ST5 MRSA (strain SA112) was compared with ST5 MRSA from humans—the main lineage found in Rio de Janeiro hospitals—to gain insights in the origin of this dog isolate. Phylogenetic analysis situated the canine genome and human strain CR14-035 in the same clade. Comparative genomics revealed similar virulence profiles for SA112 and CR14-035. Both genomes carry S. aureus genomic islands νSAα, νSAβ, and νSAγ. The virulence potential of the canine and human strains was similar in a Caenorhabditis elegans model. Together, these results suggest a potential of canine MRSA to infect humans and vice versa. The circulation in community settings of a MRSA lineage commonly found in hospitals is an additional challenge for public health surveillance authorities.Bruno PennaMarcella B. SilvaAndré E. R. SoaresAna T. R. VasconcelosMariana S. RamundoFabienne A. FerreiraMaria C. Silva-CarvalhoViviane S. de SousaRenata F. RabelloPaula T. BandeiraViviane S. de SouzaPaul J. PlanetOlney Vieira-da-MottaAna M. N. BotelhoAgnes M. S. FigueiredoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bruno Penna Marcella B. Silva André E. R. Soares Ana T. R. Vasconcelos Mariana S. Ramundo Fabienne A. Ferreira Maria C. Silva-Carvalho Viviane S. de Sousa Renata F. Rabello Paula T. Bandeira Viviane S. de Souza Paul J. Planet Olney Vieira-da-Motta Ana M. N. Botelho Agnes M. S. Figueiredo Comparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire
description	Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen associated with a wide variety of infections in humans. The ability of MRSA to infect companion animals has gained increasing attention in the scientific literature. In this study, 334 dogs were screened for MRSA in two cities located in Rio de Janeiro State. The prevalence of MRSA in dogs was 2.7%. Genotyping revealed isolates from sequence types (ST) 1, 5, 30, and 239 either colonizing or infecting dogs. The genome of the canine ST5 MRSA (strain SA112) was compared with ST5 MRSA from humans—the main lineage found in Rio de Janeiro hospitals—to gain insights in the origin of this dog isolate. Phylogenetic analysis situated the canine genome and human strain CR14-035 in the same clade. Comparative genomics revealed similar virulence profiles for SA112 and CR14-035. Both genomes carry S. aureus genomic islands νSAα, νSAβ, and νSAγ. The virulence potential of the canine and human strains was similar in a Caenorhabditis elegans model. Together, these results suggest a potential of canine MRSA to infect humans and vice versa. The circulation in community settings of a MRSA lineage commonly found in hospitals is an additional challenge for public health surveillance authorities.
format	article
author	Bruno Penna Marcella B. Silva André E. R. Soares Ana T. R. Vasconcelos Mariana S. Ramundo Fabienne A. Ferreira Maria C. Silva-Carvalho Viviane S. de Sousa Renata F. Rabello Paula T. Bandeira Viviane S. de Souza Paul J. Planet Olney Vieira-da-Motta Ana M. N. Botelho Agnes M. S. Figueiredo
author_facet	Bruno Penna Marcella B. Silva André E. R. Soares Ana T. R. Vasconcelos Mariana S. Ramundo Fabienne A. Ferreira Maria C. Silva-Carvalho Viviane S. de Sousa Renata F. Rabello Paula T. Bandeira Viviane S. de Souza Paul J. Planet Olney Vieira-da-Motta Ana M. N. Botelho Agnes M. S. Figueiredo
author_sort	Bruno Penna
title	Comparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire
title_short	Comparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire
title_full	Comparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire
title_fullStr	Comparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire
title_full_unstemmed	Comparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire
title_sort	comparative genomics of mrsa strains from human and canine origins reveals similar virulence gene repertoire
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a2a61180361042cc865f912cd9c57bbf
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Comparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire

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