Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering.

Red blood cells (RBCs) present unique reversible shape deformability, essential for both function and survival, resulting notably in cell membrane fluctuations (CMF). These CMF have been subject of many studies in order to obtain a better understanding of these remarkable biomechanical membrane prop...

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Autores principales:	Daniel Boss, Annick Hoffmann, Benjamin Rappaz, Christian Depeursinge, Pierre J Magistretti, Dimitri Van de Ville, Pierre Marquet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2a73814faf54d73b19e5853e25650f6
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spelling	oai:doaj.org-article:a2a73814faf54d73b19e5853e25650f62021-11-18T07:09:12ZSpatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering.1932-620310.1371/journal.pone.0040667https://doaj.org/article/a2a73814faf54d73b19e5853e25650f62012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22899990/?tool=EBIhttps://doaj.org/toc/1932-6203Red blood cells (RBCs) present unique reversible shape deformability, essential for both function and survival, resulting notably in cell membrane fluctuations (CMF). These CMF have been subject of many studies in order to obtain a better understanding of these remarkable biomechanical membrane properties altered in some pathological states including blood diseases. In particular the discussion over the thermal or metabolic origin of the CMF has led in the past to a large number of investigations and modeling. However, the origin of the CMF is still debated. In this article, we present an analysis of the CMF of RBCs by combining digital holographic microscopy (DHM) with an orthogonal subspace decomposition of the imaging data. These subspace components can be reliably identified and quantified as the eigenmode basis of CMF that minimizes the deformation energy of the RBC structure. By fitting the observed fluctuation modes with a theoretical dynamic model, we find that the CMF are mainly governed by the bending elasticity of the membrane and that shear and tension elasticities have only a marginal influence on the membrane fluctations of the discocyte RBC. Further, our experiments show that the role of ATP as a driving force of CMF is questionable. ATP, however, seems to be required to maintain the unique biomechanical properties of the RBC membrane that lead to thermally excited CMF.Daniel BossAnnick HoffmannBenjamin RappazChristian DepeursingePierre J MagistrettiDimitri Van de VillePierre MarquetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e40667 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Boss Annick Hoffmann Benjamin Rappaz Christian Depeursinge Pierre J Magistretti Dimitri Van de Ville Pierre Marquet Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering.
description	Red blood cells (RBCs) present unique reversible shape deformability, essential for both function and survival, resulting notably in cell membrane fluctuations (CMF). These CMF have been subject of many studies in order to obtain a better understanding of these remarkable biomechanical membrane properties altered in some pathological states including blood diseases. In particular the discussion over the thermal or metabolic origin of the CMF has led in the past to a large number of investigations and modeling. However, the origin of the CMF is still debated. In this article, we present an analysis of the CMF of RBCs by combining digital holographic microscopy (DHM) with an orthogonal subspace decomposition of the imaging data. These subspace components can be reliably identified and quantified as the eigenmode basis of CMF that minimizes the deformation energy of the RBC structure. By fitting the observed fluctuation modes with a theoretical dynamic model, we find that the CMF are mainly governed by the bending elasticity of the membrane and that shear and tension elasticities have only a marginal influence on the membrane fluctations of the discocyte RBC. Further, our experiments show that the role of ATP as a driving force of CMF is questionable. ATP, however, seems to be required to maintain the unique biomechanical properties of the RBC membrane that lead to thermally excited CMF.
format	article
author	Daniel Boss Annick Hoffmann Benjamin Rappaz Christian Depeursinge Pierre J Magistretti Dimitri Van de Ville Pierre Marquet
author_facet	Daniel Boss Annick Hoffmann Benjamin Rappaz Christian Depeursinge Pierre J Magistretti Dimitri Van de Ville Pierre Marquet
author_sort	Daniel Boss
title	Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering.
title_short	Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering.
title_full	Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering.
title_fullStr	Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering.
title_full_unstemmed	Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering.
title_sort	spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of atp in flickering.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a2a73814faf54d73b19e5853e25650f6
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Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering.

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