A novel locus (Bnsdt2) in a TFL1 homologue sustaining determinate growth in Brassica napus

Abstract Background The determinate growth habits is beneficial for plant architecture modification and the development of crops cultivars suited to mechanized production systems. Which play an important role in the genetic improvement of crops. In Brassica napus, a determinate inflorescence strain...

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Autores principales:	Kaixiang Li, Liang Xu, Yongpeng Jia, Cuiping Chen, Yanmei Yao, Haidong Liu, Dezhi Du
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Brassica napus Determinate inflorescence Agronomic traits Bnsdt2 qRT-PCR Botany QK1-989
Acceso en línea:	https://doaj.org/article/a2aa8fadf5894288a9c78b57693b0601
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spelling	oai:doaj.org-article:a2aa8fadf5894288a9c78b57693b06012021-12-05T12:06:20ZA novel locus (Bnsdt2) in a TFL1 homologue sustaining determinate growth in Brassica napus10.1186/s12870-021-03348-01471-2229https://doaj.org/article/a2aa8fadf5894288a9c78b57693b06012021-12-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03348-0https://doaj.org/toc/1471-2229Abstract Background The determinate growth habits is beneficial for plant architecture modification and the development of crops cultivars suited to mechanized production systems. Which play an important role in the genetic improvement of crops. In Brassica napus, a determinate inflorescence strain (4769) has been discovered among doubled haploid (DH) lines obtained from a spring B. napus × winter B. napus cross, but there are few reports on it. We fine mapped a determinate inflorescence locus, and evaluated the effect of the determinate growth habit on agronomic traits. Results In this study, we assessed the effect of the determinate growth habit on agronomic traits. The results showed that determinacy is beneficial for reducing plant height and flowering time, advancing maturity, enhancing lodging resistance, increasing plant branches and maintaining productivity. Genetic analysis in the determinate (4769) and indeterminate (2982) genotypes revealed that two independently inherited recessive genes (Bnsdt1, Bnsdt2) are responsible for this determinate growth trait. Bnsdt2 was subsequently mapped in BC2 and BC3 populations derived from the combination 2982 × 4769. Bnsdt2 could be delimited to an approximately 122.9 kb region between 68,586.2 kb and 68,709.1 kb on C09. BLAST analysis of these candidate intervals showed that chrC09g006434 (BnaC09.TFL1) is homologous to TFL1 of A. thaliana. Sequence analysis of two alleles identified two non-synonymous SNPs (T136C, G141C) in the first exon of BnaC09.TFL1, resulting in two amino acid substitutions (Phe46Leu, Leu47Phe). Subsequently, qRT-PCR revealed that BnaC09.TFL1 expression in shoot apexes was significantly higher in NIL-4769 than in 4769, suggesting its essential role in sustaining the indeterminate growth habit. Conclusions In this study, the novel locus Bnsdt2, a recessive genes for determinate inflorescence in B. napus, was fine-mapped to a 68,586.2 kb - 68,709.1 kb interval on C09. The annotated genes chrC09g006434 (BnaC09.TFL1) that may be responsible for inflorescence traits were found.Kaixiang LiLiang XuYongpeng JiaCuiping ChenYanmei YaoHaidong LiuDezhi DuBMCarticleBrassica napusDeterminate inflorescenceAgronomic traitsBnsdt2qRT-PCRBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Brassica napus Determinate inflorescence Agronomic traits Bnsdt2 qRT-PCR Botany QK1-989
spellingShingle	Brassica napus Determinate inflorescence Agronomic traits Bnsdt2 qRT-PCR Botany QK1-989 Kaixiang Li Liang Xu Yongpeng Jia Cuiping Chen Yanmei Yao Haidong Liu Dezhi Du A novel locus (Bnsdt2) in a TFL1 homologue sustaining determinate growth in Brassica napus
description	Abstract Background The determinate growth habits is beneficial for plant architecture modification and the development of crops cultivars suited to mechanized production systems. Which play an important role in the genetic improvement of crops. In Brassica napus, a determinate inflorescence strain (4769) has been discovered among doubled haploid (DH) lines obtained from a spring B. napus × winter B. napus cross, but there are few reports on it. We fine mapped a determinate inflorescence locus, and evaluated the effect of the determinate growth habit on agronomic traits. Results In this study, we assessed the effect of the determinate growth habit on agronomic traits. The results showed that determinacy is beneficial for reducing plant height and flowering time, advancing maturity, enhancing lodging resistance, increasing plant branches and maintaining productivity. Genetic analysis in the determinate (4769) and indeterminate (2982) genotypes revealed that two independently inherited recessive genes (Bnsdt1, Bnsdt2) are responsible for this determinate growth trait. Bnsdt2 was subsequently mapped in BC2 and BC3 populations derived from the combination 2982 × 4769. Bnsdt2 could be delimited to an approximately 122.9 kb region between 68,586.2 kb and 68,709.1 kb on C09. BLAST analysis of these candidate intervals showed that chrC09g006434 (BnaC09.TFL1) is homologous to TFL1 of A. thaliana. Sequence analysis of two alleles identified two non-synonymous SNPs (T136C, G141C) in the first exon of BnaC09.TFL1, resulting in two amino acid substitutions (Phe46Leu, Leu47Phe). Subsequently, qRT-PCR revealed that BnaC09.TFL1 expression in shoot apexes was significantly higher in NIL-4769 than in 4769, suggesting its essential role in sustaining the indeterminate growth habit. Conclusions In this study, the novel locus Bnsdt2, a recessive genes for determinate inflorescence in B. napus, was fine-mapped to a 68,586.2 kb - 68,709.1 kb interval on C09. The annotated genes chrC09g006434 (BnaC09.TFL1) that may be responsible for inflorescence traits were found.
format	article
author	Kaixiang Li Liang Xu Yongpeng Jia Cuiping Chen Yanmei Yao Haidong Liu Dezhi Du
author_facet	Kaixiang Li Liang Xu Yongpeng Jia Cuiping Chen Yanmei Yao Haidong Liu Dezhi Du
author_sort	Kaixiang Li
title	A novel locus (Bnsdt2) in a TFL1 homologue sustaining determinate growth in Brassica napus
title_short	A novel locus (Bnsdt2) in a TFL1 homologue sustaining determinate growth in Brassica napus
title_full	A novel locus (Bnsdt2) in a TFL1 homologue sustaining determinate growth in Brassica napus
title_fullStr	A novel locus (Bnsdt2) in a TFL1 homologue sustaining determinate growth in Brassica napus
title_full_unstemmed	A novel locus (Bnsdt2) in a TFL1 homologue sustaining determinate growth in Brassica napus
title_sort	novel locus (bnsdt2) in a tfl1 homologue sustaining determinate growth in brassica napus
publisher	BMC
publishDate	2021
url	https://doaj.org/article/a2aa8fadf5894288a9c78b57693b0601
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A novel locus (Bnsdt2) in a TFL1 homologue sustaining determinate growth in Brassica napus

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