The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism

Wanzhen Li,1,* Ping Song,1,* Ying Xin,1 Zhao Kuang,1 Qin Liu,1 Fei Ge,1 Longbao Zhu,1 Xuguang Zhang,2 Yugui Tao,1 weiwei zhang1,2 1School of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui, 241000, People’s Republic of China; 2Bankpeptide Biological Technology Co...

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Autores principales:	Li W, Song P, Xin Y, Kuang Z, Liu Q, Ge F, Zhu L, Zhang X, Tao Y, zhang W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	antibacterial peptides cdse qd antibacterial mechanism rnaseq in vitro and in vivo Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a2b0d5cb7a464408b372dc784897cfcf
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spelling	oai:doaj.org-article:a2b0d5cb7a464408b372dc784897cfcf2021-12-02T16:00:18ZThe Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism1178-2013https://doaj.org/article/a2b0d5cb7a464408b372dc784897cfcf2021-03-01T00:00:00Zhttps://www.dovepress.com/the-effects-of-luminescent-cdse-quantum-dot-functionalized-antimicrobi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wanzhen Li,1,* Ping Song,1,* Ying Xin,1 Zhao Kuang,1 Qin Liu,1 Fei Ge,1 Longbao Zhu,1 Xuguang Zhang,2 Yugui Tao,1 weiwei zhang1,2 1School of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui, 241000, People’s Republic of China; 2Bankpeptide Biological Technology Company, Hefei, Anhui, 230031, People’s Republic of China*These authors contributed equally to this workCorrespondence: weiwei zhang Email zwwjcf0908@163.comBackground: With the development of bacterial resistance, the range of effective antibiotics is increasingly becoming more limited. The effective use of nanoscale antimicrobial peptides (AP) in therapeutic and diagnostic methods is a strategy for new antibiotics.Methods: Combining both AP and cadmium selenide (CdSe) into a composite material may result in a reagent with novel properties, such as enhanced antibacterial activity, fluorescence and favorable stability in aqueous solution.Results: AP-loaded CdSe NPs (AP-CdSe NPs) showed strong antibacterial activity against multidrug-resistant (MDR) Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in vitro and in vivo. Colony-forming unit (CFU) and minimum inhibitory concentration (MIC) assays showed that AP-CdSe NPs have highly effective antibacterial activity. The quantitative analysis of apoptosis by flow cytometry analysis further confirmed that MDR E. coli and S. aureus treated with AP-CdSe NPs had death rates of 98.76% and 99.13%, respectively. Also, AP-CdSe NPs was found to inhibit bacterial activity in an in vivo bacteremia model in mice infected with S. aureus. In addition, the antibacterial mechanism of AP-CdSe NPs was determined by RNA sequencing analysis. Gene ontology (GO) analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis revealed the molecular mechanism of the antibacterial effect of AP-CdSe NPs. Importantly, histopathology analysis, and hematological toxicity analysis indicated that AP-CdSe NPs had few side effects.Conclusion: These results demonstrate that AP loaded on CdSe NPs had a higher water solubility, bioavailability and antibacterial effect compared with raw AP. This study reports findings that are helpful for the design and development of antibacterial treatment strategies based on AP.Keywords: antibacterial peptides, CdSe QD, antibacterial mechanism, RNASeq, in vitro and in vivoLi WSong PXin YKuang ZLiu QGe FZhu LZhang XTao Yzhang WDove Medical Pressarticleantibacterial peptidescdse qdantibacterial mechanismrnaseqin vitro and in vivoMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 1849-1867 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibacterial peptides cdse qd antibacterial mechanism rnaseq in vitro and in vivo Medicine (General) R5-920
spellingShingle	antibacterial peptides cdse qd antibacterial mechanism rnaseq in vitro and in vivo Medicine (General) R5-920 Li W Song P Xin Y Kuang Z Liu Q Ge F Zhu L Zhang X Tao Y zhang W The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism
description	Wanzhen Li,1,* Ping Song,1,* Ying Xin,1 Zhao Kuang,1 Qin Liu,1 Fei Ge,1 Longbao Zhu,1 Xuguang Zhang,2 Yugui Tao,1 weiwei zhang1,2 1School of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui, 241000, People’s Republic of China; 2Bankpeptide Biological Technology Company, Hefei, Anhui, 230031, People’s Republic of China*These authors contributed equally to this workCorrespondence: weiwei zhang Email zwwjcf0908@163.comBackground: With the development of bacterial resistance, the range of effective antibiotics is increasingly becoming more limited. The effective use of nanoscale antimicrobial peptides (AP) in therapeutic and diagnostic methods is a strategy for new antibiotics.Methods: Combining both AP and cadmium selenide (CdSe) into a composite material may result in a reagent with novel properties, such as enhanced antibacterial activity, fluorescence and favorable stability in aqueous solution.Results: AP-loaded CdSe NPs (AP-CdSe NPs) showed strong antibacterial activity against multidrug-resistant (MDR) Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in vitro and in vivo. Colony-forming unit (CFU) and minimum inhibitory concentration (MIC) assays showed that AP-CdSe NPs have highly effective antibacterial activity. The quantitative analysis of apoptosis by flow cytometry analysis further confirmed that MDR E. coli and S. aureus treated with AP-CdSe NPs had death rates of 98.76% and 99.13%, respectively. Also, AP-CdSe NPs was found to inhibit bacterial activity in an in vivo bacteremia model in mice infected with S. aureus. In addition, the antibacterial mechanism of AP-CdSe NPs was determined by RNA sequencing analysis. Gene ontology (GO) analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis revealed the molecular mechanism of the antibacterial effect of AP-CdSe NPs. Importantly, histopathology analysis, and hematological toxicity analysis indicated that AP-CdSe NPs had few side effects.Conclusion: These results demonstrate that AP loaded on CdSe NPs had a higher water solubility, bioavailability and antibacterial effect compared with raw AP. This study reports findings that are helpful for the design and development of antibacterial treatment strategies based on AP.Keywords: antibacterial peptides, CdSe QD, antibacterial mechanism, RNASeq, in vitro and in vivo
format	article
author	Li W Song P Xin Y Kuang Z Liu Q Ge F Zhu L Zhang X Tao Y zhang W
author_facet	Li W Song P Xin Y Kuang Z Liu Q Ge F Zhu L Zhang X Tao Y zhang W
author_sort	Li W
title	The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism
title_short	The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism
title_full	The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism
title_fullStr	The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism
title_full_unstemmed	The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism
title_sort	effects of luminescent cdse quantum dot-functionalized antimicrobial peptides nanoparticles on antibacterial activity and molecular mechanism
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/a2b0d5cb7a464408b372dc784897cfcf
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The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism

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