Impact of Escherichia coli probiotic strains ED1a and Nissle 1917 on the excretion and gut carriage of extended-spectrum beta-lactamase-producing E. coli in pigs

We evaluated the impact of the administration of two Escherichia coli probiotic strains (ED1a and Nissle 1917) to pigs on the gut carriage or shedding of extended-spectrum beta-lactamase-producing E. coli. The probiotics were given to four sows from 12 days before farrowing to the weaning day, and t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gwénaëlle Mourand, Frédéric Paboeuf, Pauline Grippon, Pierrick Lucas, Stéphanie Bougeard, Erick Denamur, Isabelle Kempf
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	E. coli Probiotic Cephalosporin resistance Pig Microbiota Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/a2b4d37178e94efeb2dc442ce67f4b17
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a2b4d37178e94efeb2dc442ce67f4b17
record_format	dspace
spelling	oai:doaj.org-article:a2b4d37178e94efeb2dc442ce67f4b172021-11-14T04:34:44ZImpact of Escherichia coli probiotic strains ED1a and Nissle 1917 on the excretion and gut carriage of extended-spectrum beta-lactamase-producing E. coli in pigs2451-943X10.1016/j.vas.2021.100217https://doaj.org/article/a2b4d37178e94efeb2dc442ce67f4b172021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2451943X21000521https://doaj.org/toc/2451-943XWe evaluated the impact of the administration of two Escherichia coli probiotic strains (ED1a and Nissle 1917) to pigs on the gut carriage or shedding of extended-spectrum beta-lactamase-producing E. coli. The probiotics were given to four sows from 12 days before farrowing to the weaning day, and to the 23 piglets (infected treated group (IPro)) from birth to the age of 49 days. Four other sows and their 24 piglets (infected non-treated group (INT)) did not receive the probiotics. IPro and INT piglets (n = 47) were orally inoculated with the strain E. coli 17–348F-RifR carrying the blaCTX−M-1 gene and resistant to rifampicin. Cefotaxime-resistant (CTXR) E. coli and rifampicin-resistant (RifR) E. coli were cultured and excretion of probiotics was studied using PCR on individual faecal and post-mortem samples, and from manure collected after the challenge with resistant E. coli. CTXR and RifR E. coli isolates were characterized to detect transfer of the blaCTX−M-1 to other strains.. Overall, there was no significant reduction in faecal excretion of CTXR and RifR E. coli in IPro pigs compared with INT pigs, although the CTXR and RifR E. coli titres were slightly, but significantly lower in the colon, caecum and rectum at post mortem. Excretion of the probiotics decreased with age, but Nissle 1917 was detected in most pigs at post-mortem. No transfer of the blaCTX−M-1 gene to probiotic and other E. coli strains was detected. In conclusion, in our experimental conditions, the used probiotics did not reduce shedding of the challenge strain.Gwénaëlle MourandFrédéric PaboeufPauline GripponPierrick LucasStéphanie BougeardErick DenamurIsabelle KempfElsevierarticleE. coliProbioticCephalosporin resistancePigMicrobiotaVeterinary medicineSF600-1100ENVeterinary and Animal Science, Vol 14, Iss , Pp 100217- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	E. coli Probiotic Cephalosporin resistance Pig Microbiota Veterinary medicine SF600-1100
spellingShingle	E. coli Probiotic Cephalosporin resistance Pig Microbiota Veterinary medicine SF600-1100 Gwénaëlle Mourand Frédéric Paboeuf Pauline Grippon Pierrick Lucas Stéphanie Bougeard Erick Denamur Isabelle Kempf Impact of Escherichia coli probiotic strains ED1a and Nissle 1917 on the excretion and gut carriage of extended-spectrum beta-lactamase-producing E. coli in pigs
description	We evaluated the impact of the administration of two Escherichia coli probiotic strains (ED1a and Nissle 1917) to pigs on the gut carriage or shedding of extended-spectrum beta-lactamase-producing E. coli. The probiotics were given to four sows from 12 days before farrowing to the weaning day, and to the 23 piglets (infected treated group (IPro)) from birth to the age of 49 days. Four other sows and their 24 piglets (infected non-treated group (INT)) did not receive the probiotics. IPro and INT piglets (n = 47) were orally inoculated with the strain E. coli 17–348F-RifR carrying the blaCTX−M-1 gene and resistant to rifampicin. Cefotaxime-resistant (CTXR) E. coli and rifampicin-resistant (RifR) E. coli were cultured and excretion of probiotics was studied using PCR on individual faecal and post-mortem samples, and from manure collected after the challenge with resistant E. coli. CTXR and RifR E. coli isolates were characterized to detect transfer of the blaCTX−M-1 to other strains.. Overall, there was no significant reduction in faecal excretion of CTXR and RifR E. coli in IPro pigs compared with INT pigs, although the CTXR and RifR E. coli titres were slightly, but significantly lower in the colon, caecum and rectum at post mortem. Excretion of the probiotics decreased with age, but Nissle 1917 was detected in most pigs at post-mortem. No transfer of the blaCTX−M-1 gene to probiotic and other E. coli strains was detected. In conclusion, in our experimental conditions, the used probiotics did not reduce shedding of the challenge strain.
format	article
author	Gwénaëlle Mourand Frédéric Paboeuf Pauline Grippon Pierrick Lucas Stéphanie Bougeard Erick Denamur Isabelle Kempf
author_facet	Gwénaëlle Mourand Frédéric Paboeuf Pauline Grippon Pierrick Lucas Stéphanie Bougeard Erick Denamur Isabelle Kempf
author_sort	Gwénaëlle Mourand
title	Impact of Escherichia coli probiotic strains ED1a and Nissle 1917 on the excretion and gut carriage of extended-spectrum beta-lactamase-producing E. coli in pigs
title_short	Impact of Escherichia coli probiotic strains ED1a and Nissle 1917 on the excretion and gut carriage of extended-spectrum beta-lactamase-producing E. coli in pigs
title_full	Impact of Escherichia coli probiotic strains ED1a and Nissle 1917 on the excretion and gut carriage of extended-spectrum beta-lactamase-producing E. coli in pigs
title_fullStr	Impact of Escherichia coli probiotic strains ED1a and Nissle 1917 on the excretion and gut carriage of extended-spectrum beta-lactamase-producing E. coli in pigs
title_full_unstemmed	Impact of Escherichia coli probiotic strains ED1a and Nissle 1917 on the excretion and gut carriage of extended-spectrum beta-lactamase-producing E. coli in pigs
title_sort	impact of escherichia coli probiotic strains ed1a and nissle 1917 on the excretion and gut carriage of extended-spectrum beta-lactamase-producing e. coli in pigs
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/a2b4d37178e94efeb2dc442ce67f4b17
work_keys_str_mv	AT gwenaellemourand impactofescherichiacoliprobioticstrainsed1aandnissle1917ontheexcretionandgutcarriageofextendedspectrumbetalactamaseproducingecoliinpigs AT fredericpaboeuf impactofescherichiacoliprobioticstrainsed1aandnissle1917ontheexcretionandgutcarriageofextendedspectrumbetalactamaseproducingecoliinpigs AT paulinegrippon impactofescherichiacoliprobioticstrainsed1aandnissle1917ontheexcretionandgutcarriageofextendedspectrumbetalactamaseproducingecoliinpigs AT pierricklucas impactofescherichiacoliprobioticstrainsed1aandnissle1917ontheexcretionandgutcarriageofextendedspectrumbetalactamaseproducingecoliinpigs AT stephaniebougeard impactofescherichiacoliprobioticstrainsed1aandnissle1917ontheexcretionandgutcarriageofextendedspectrumbetalactamaseproducingecoliinpigs AT erickdenamur impactofescherichiacoliprobioticstrainsed1aandnissle1917ontheexcretionandgutcarriageofextendedspectrumbetalactamaseproducingecoliinpigs AT isabellekempf impactofescherichiacoliprobioticstrainsed1aandnissle1917ontheexcretionandgutcarriageofextendedspectrumbetalactamaseproducingecoliinpigs
_version_	1718429914835714048

Impact of Escherichia coli probiotic strains ED1a and Nissle 1917 on the excretion and gut carriage of extended-spectrum beta-lactamase-producing E. coli in pigs

Ejemplares similares