Antifungal Activity of Biocontrol Agents In Vitro and Potential Application to Reduce Mycotoxins (Aflatoxin B1 and Ochratoxin A)

Food bio-preservatives are requested as substituents of chemical pesticides in food. The aim of this study was to carry out a screening of twenty biocontrol agents (BCAs) for their potential fungicidal activity in vitro. Twenty BCAs were tested against ten pathogenic fungi. Some of the cell-free sup...

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Autores principales:	Francisco Illueca, Pilar Vila-Donat, Jorge Calpe, Carlos Luz, Giuseppe Meca, Juan M. Quiles
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biocontrol agents antifungal mycotoxins reduction in vitro bio-preservation Medicine R
Acceso en línea:	https://doaj.org/article/a2bc8d76b2de41819d2240af009a9f0b
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spelling	oai:doaj.org-article:a2bc8d76b2de41819d2240af009a9f0b2021-11-25T19:08:33ZAntifungal Activity of Biocontrol Agents In Vitro and Potential Application to Reduce Mycotoxins (Aflatoxin B1 and Ochratoxin A)10.3390/toxins131107522072-6651https://doaj.org/article/a2bc8d76b2de41819d2240af009a9f0b2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/752https://doaj.org/toc/2072-6651Food bio-preservatives are requested as substituents of chemical pesticides in food. The aim of this study was to carry out a screening of twenty biocontrol agents (BCAs) for their potential fungicidal activity in vitro. Twenty BCAs were tested against ten pathogenic fungi. Some of the cell-free supernatants (CFS) tested showed in vitro antifungal activity versus pathogenic fungi. The highest fungicidal activity was observed in the fermented CFS of <i>Paenibacillus chibensis</i> CECT 375, <i>Bacillus amyloliquefaciens</i> CECT 493, and <i>Pantoea agglomerans</i> CECT 850, which showed a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of 125 and 250 g/L, respectively. The compounds responsible for the antifungal activity, such as organic and phenolic acids, were determined. Lactic acid, acetic acid, benzoic acid, and phenyllactic acid among others can be related to antifungal activity. HPLC-MS/MS analysis showed a reduction of ochratoxin A (OTA) and aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) up to 26% (<i>Paenibacillus alvei</i> CECT 2) and 55% (<i>Paenibacillus polymyxa</i> CECT 155), respectively. The present study prompts that metabolism products of BCAs are propitious for the bioconservation of food, due to their ability to reduce the proliferation of mycotoxigenic fungi and mycotoxins production.Francisco IlluecaPilar Vila-DonatJorge CalpeCarlos LuzGiuseppe MecaJuan M. QuilesMDPI AGarticlebiocontrol agentsantifungalmycotoxinsreductionin vitrobio-preservationMedicineRENToxins, Vol 13, Iss 752, p 752 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biocontrol agents antifungal mycotoxins reduction in vitro bio-preservation Medicine R
spellingShingle	biocontrol agents antifungal mycotoxins reduction in vitro bio-preservation Medicine R Francisco Illueca Pilar Vila-Donat Jorge Calpe Carlos Luz Giuseppe Meca Juan M. Quiles Antifungal Activity of Biocontrol Agents In Vitro and Potential Application to Reduce Mycotoxins (Aflatoxin B1 and Ochratoxin A)
description	Food bio-preservatives are requested as substituents of chemical pesticides in food. The aim of this study was to carry out a screening of twenty biocontrol agents (BCAs) for their potential fungicidal activity in vitro. Twenty BCAs were tested against ten pathogenic fungi. Some of the cell-free supernatants (CFS) tested showed in vitro antifungal activity versus pathogenic fungi. The highest fungicidal activity was observed in the fermented CFS of <i>Paenibacillus chibensis</i> CECT 375, <i>Bacillus amyloliquefaciens</i> CECT 493, and <i>Pantoea agglomerans</i> CECT 850, which showed a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of 125 and 250 g/L, respectively. The compounds responsible for the antifungal activity, such as organic and phenolic acids, were determined. Lactic acid, acetic acid, benzoic acid, and phenyllactic acid among others can be related to antifungal activity. HPLC-MS/MS analysis showed a reduction of ochratoxin A (OTA) and aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) up to 26% (<i>Paenibacillus alvei</i> CECT 2) and 55% (<i>Paenibacillus polymyxa</i> CECT 155), respectively. The present study prompts that metabolism products of BCAs are propitious for the bioconservation of food, due to their ability to reduce the proliferation of mycotoxigenic fungi and mycotoxins production.
format	article
author	Francisco Illueca Pilar Vila-Donat Jorge Calpe Carlos Luz Giuseppe Meca Juan M. Quiles
author_facet	Francisco Illueca Pilar Vila-Donat Jorge Calpe Carlos Luz Giuseppe Meca Juan M. Quiles
author_sort	Francisco Illueca
title	Antifungal Activity of Biocontrol Agents In Vitro and Potential Application to Reduce Mycotoxins (Aflatoxin B1 and Ochratoxin A)
title_short	Antifungal Activity of Biocontrol Agents In Vitro and Potential Application to Reduce Mycotoxins (Aflatoxin B1 and Ochratoxin A)
title_full	Antifungal Activity of Biocontrol Agents In Vitro and Potential Application to Reduce Mycotoxins (Aflatoxin B1 and Ochratoxin A)
title_fullStr	Antifungal Activity of Biocontrol Agents In Vitro and Potential Application to Reduce Mycotoxins (Aflatoxin B1 and Ochratoxin A)
title_full_unstemmed	Antifungal Activity of Biocontrol Agents In Vitro and Potential Application to Reduce Mycotoxins (Aflatoxin B1 and Ochratoxin A)
title_sort	antifungal activity of biocontrol agents in vitro and potential application to reduce mycotoxins (aflatoxin b1 and ochratoxin a)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a2bc8d76b2de41819d2240af009a9f0b
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Antifungal Activity of Biocontrol Agents In Vitro and Potential Application to Reduce Mycotoxins (Aflatoxin B1 and Ochratoxin A)

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