Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system

Abstract Zebra finch is a representative animal model for studying the molecular basis of human disorders of vocal development and communication. Accordingly, various functional studies of zebra finch have knocked down or introduced foreign genes in vivo; however, their germline transmission efficie...

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Autores principales:	Kyung Min Jung, Young Min Kim, Jin Lee Kim, Jae Yong Han
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2c4da9100574963b0b0232488f3a571
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spelling	oai:doaj.org-article:a2c4da9100574963b0b0232488f3a5712021-12-02T16:17:28ZEfficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system10.1038/s41598-021-94229-x2045-2322https://doaj.org/article/a2c4da9100574963b0b0232488f3a5712021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94229-xhttps://doaj.org/toc/2045-2322Abstract Zebra finch is a representative animal model for studying the molecular basis of human disorders of vocal development and communication. Accordingly, various functional studies of zebra finch have knocked down or introduced foreign genes in vivo; however, their germline transmission efficiency is remarkably low. The primordial germ cell (PGC)-mediated method is preferred for avian transgenic studies; however, use of this method is restricted in zebra finch due to the lack of an efficient gene transfer method for the germline. To target primary germ cells that are difficult to transfect and manipulate, an adenovirus-mediated gene transfer system with high efficiency in a wide range of cell types may be useful. Here, we isolated and characterized two types of primary germline-competent stem cells, PGCs and spermatogonial stem cells (SSCs), from embryonic and adult reproductive tissues of zebra finch and demonstrated that genes were most efficiently transferred into these cells using an adenovirus-mediated system. This system was successfully used to generate gene-edited PGCs in vitro. These results are expected to improve transgenic zebra finch production.Kyung Min JungYoung Min KimJin Lee KimJae Yong HanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kyung Min Jung Young Min Kim Jin Lee Kim Jae Yong Han Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
description	Abstract Zebra finch is a representative animal model for studying the molecular basis of human disorders of vocal development and communication. Accordingly, various functional studies of zebra finch have knocked down or introduced foreign genes in vivo; however, their germline transmission efficiency is remarkably low. The primordial germ cell (PGC)-mediated method is preferred for avian transgenic studies; however, use of this method is restricted in zebra finch due to the lack of an efficient gene transfer method for the germline. To target primary germ cells that are difficult to transfect and manipulate, an adenovirus-mediated gene transfer system with high efficiency in a wide range of cell types may be useful. Here, we isolated and characterized two types of primary germline-competent stem cells, PGCs and spermatogonial stem cells (SSCs), from embryonic and adult reproductive tissues of zebra finch and demonstrated that genes were most efficiently transferred into these cells using an adenovirus-mediated system. This system was successfully used to generate gene-edited PGCs in vitro. These results are expected to improve transgenic zebra finch production.
format	article
author	Kyung Min Jung Young Min Kim Jin Lee Kim Jae Yong Han
author_facet	Kyung Min Jung Young Min Kim Jin Lee Kim Jae Yong Han
author_sort	Kyung Min Jung
title	Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
title_short	Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
title_full	Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
title_fullStr	Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
title_full_unstemmed	Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
title_sort	efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a2c4da9100574963b0b0232488f3a571
work_keys_str_mv	AT kyungminjung efficientgenetransferintozebrafinchgermlinecompetentstemcellsusinganadenoviralvectorsystem AT youngminkim efficientgenetransferintozebrafinchgermlinecompetentstemcellsusinganadenoviralvectorsystem AT jinleekim efficientgenetransferintozebrafinchgermlinecompetentstemcellsusinganadenoviralvectorsystem AT jaeyonghan efficientgenetransferintozebrafinchgermlinecompetentstemcellsusinganadenoviralvectorsystem
_version_	1718384247849353216

Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system

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