Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system.

The developmental competence of male and female gametes is frequently reduced under in vitro conditions, mainly due to oxidative stress during handling. The amino-acid derived hormone melatonin has emerged as a potent non-enzymatic antioxidant in many biological systems. The goal of the present stud...

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Autores principales:	Juan Carlos Gutiérrez-Añez, Heiko Henning, Andrea Lucas-Hahn, Ulrich Baulain, Patrick Aldag, Birgit Sieg, Vivian Hensel, Doris Herrmann, Heiner Niemann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2cfd0fb620d49e5a9aa999ed72bd4ae
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spelling	oai:doaj.org-article:a2cfd0fb620d49e5a9aa999ed72bd4ae2021-12-02T20:08:37ZMelatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system.1932-620310.1371/journal.pone.0256701https://doaj.org/article/a2cfd0fb620d49e5a9aa999ed72bd4ae2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256701https://doaj.org/toc/1932-6203The developmental competence of male and female gametes is frequently reduced under in vitro conditions, mainly due to oxidative stress during handling. The amino-acid derived hormone melatonin has emerged as a potent non-enzymatic antioxidant in many biological systems. The goal of the present study was to evaluate the effects of melatonin on post-thaw sperm quality, fertilizing ability, and embryo development and competence in vitro after in vitro fertilization. Frozen-thawed bovine spermatozoa were incubated either in the presence of 10-11 M melatonin (MT), or its solvent (ethanol; Sham-Control), or plain Tyrode's Albumin Lactate Pyruvate medium (TALP, Control). Computer-Assisted Sperm Analysis (CASA) and flow cytometry data after 30 min, 120 min, and 180 min incubation did not reveal any significant effects of melatonin on average motility parameters, sperm subpopulation structure as determined by hierarchical cluster, or on the percentage of viable, acrosome intact sperm, or viable sperm with active mitochondria. Nevertheless, in vitro matured cumulus-oocyte-complexes fertilized with spermatozoa which had been preincubated with 10-11 M melatonin (MT-Sperm) showed higher (P < 0.01) rates of monospermic fertilization, reduced (P < 0.05) polyspermy and enhanced (P < 0.05) embryo development compared to the Control group. Moreover, the relative abundance of MAPK13 in the in vitro-derived blastocysts was greater (P < 0.05) than observed in the Control group. In conclusion, adding melatonin to the sperm-preparation protocol for bovine IVF improved proper fertilization and enhanced embryonic development and competence in vitro.Juan Carlos Gutiérrez-AñezHeiko HenningAndrea Lucas-HahnUlrich BaulainPatrick AldagBirgit SiegVivian HenselDoris HerrmannHeiner NiemannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256701 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juan Carlos Gutiérrez-Añez Heiko Henning Andrea Lucas-Hahn Ulrich Baulain Patrick Aldag Birgit Sieg Vivian Hensel Doris Herrmann Heiner Niemann Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system.
description	The developmental competence of male and female gametes is frequently reduced under in vitro conditions, mainly due to oxidative stress during handling. The amino-acid derived hormone melatonin has emerged as a potent non-enzymatic antioxidant in many biological systems. The goal of the present study was to evaluate the effects of melatonin on post-thaw sperm quality, fertilizing ability, and embryo development and competence in vitro after in vitro fertilization. Frozen-thawed bovine spermatozoa were incubated either in the presence of 10-11 M melatonin (MT), or its solvent (ethanol; Sham-Control), or plain Tyrode's Albumin Lactate Pyruvate medium (TALP, Control). Computer-Assisted Sperm Analysis (CASA) and flow cytometry data after 30 min, 120 min, and 180 min incubation did not reveal any significant effects of melatonin on average motility parameters, sperm subpopulation structure as determined by hierarchical cluster, or on the percentage of viable, acrosome intact sperm, or viable sperm with active mitochondria. Nevertheless, in vitro matured cumulus-oocyte-complexes fertilized with spermatozoa which had been preincubated with 10-11 M melatonin (MT-Sperm) showed higher (P < 0.01) rates of monospermic fertilization, reduced (P < 0.05) polyspermy and enhanced (P < 0.05) embryo development compared to the Control group. Moreover, the relative abundance of MAPK13 in the in vitro-derived blastocysts was greater (P < 0.05) than observed in the Control group. In conclusion, adding melatonin to the sperm-preparation protocol for bovine IVF improved proper fertilization and enhanced embryonic development and competence in vitro.
format	article
author	Juan Carlos Gutiérrez-Añez Heiko Henning Andrea Lucas-Hahn Ulrich Baulain Patrick Aldag Birgit Sieg Vivian Hensel Doris Herrmann Heiner Niemann
author_facet	Juan Carlos Gutiérrez-Añez Heiko Henning Andrea Lucas-Hahn Ulrich Baulain Patrick Aldag Birgit Sieg Vivian Hensel Doris Herrmann Heiner Niemann
author_sort	Juan Carlos Gutiérrez-Añez
title	Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system.
title_short	Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system.
title_full	Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system.
title_fullStr	Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system.
title_full_unstemmed	Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system.
title_sort	melatonin improves rate of monospermic fertilization and early embryo development in a bovine ivf system.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a2cfd0fb620d49e5a9aa999ed72bd4ae
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Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system.

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