A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions

Protein-protein interactions (PPIs) play a major role in defining biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-barcoding, and an analytical framework to precisely call fitness...

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Autores principales:	Ulrich Schlecht, Zhimin Liu, Jamie R. Blundell, Robert P. St.Onge, Sasha F. Levy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a2d1f7e3c1634cb29a0d1f9355ce7b11
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spelling	oai:doaj.org-article:a2d1f7e3c1634cb29a0d1f9355ce7b112021-12-02T14:42:39ZA scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions10.1038/ncomms155862041-1723https://doaj.org/article/a2d1f7e3c1634cb29a0d1f9355ce7b112017-05-01T00:00:00Zhttps://doi.org/10.1038/ncomms15586https://doaj.org/toc/2041-1723Protein-protein interactions (PPIs) play a major role in defining biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-barcoding, and an analytical framework to precisely call fitness from barcode lineage trajectories.Ulrich SchlechtZhimin LiuJamie R. BlundellRobert P. St.OngeSasha F. LevyNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ulrich Schlecht Zhimin Liu Jamie R. Blundell Robert P. St.Onge Sasha F. Levy A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
description	Protein-protein interactions (PPIs) play a major role in defining biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-barcoding, and an analytical framework to precisely call fitness from barcode lineage trajectories.
format	article
author	Ulrich Schlecht Zhimin Liu Jamie R. Blundell Robert P. St.Onge Sasha F. Levy
author_facet	Ulrich Schlecht Zhimin Liu Jamie R. Blundell Robert P. St.Onge Sasha F. Levy
author_sort	Ulrich Schlecht
title	A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
title_short	A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
title_full	A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
title_fullStr	A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
title_full_unstemmed	A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
title_sort	scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a2d1f7e3c1634cb29a0d1f9355ce7b11
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A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions

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