A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions

Protein-protein interactions (PPIs) play a major role in defining biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-barcoding, and an analytical framework to precisely call fitness...

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Autores principales: Ulrich Schlecht, Zhimin Liu, Jamie R. Blundell, Robert P. St.Onge, Sasha F. Levy
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/a2d1f7e3c1634cb29a0d1f9355ce7b11
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