A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
Protein-protein interactions (PPIs) play a major role in defining biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-barcoding, and an analytical framework to precisely call fitness...
Guardado en:
Autores principales: | Ulrich Schlecht, Zhimin Liu, Jamie R. Blundell, Robert P. St.Onge, Sasha F. Levy |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2017
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Materias: | |
Acceso en línea: | https://doaj.org/article/a2d1f7e3c1634cb29a0d1f9355ce7b11 |
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