Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA

Abstract In the present study lipopeptide biosurfactant with high emulsification capacity produced by human skin bacterium Paenibacillus thiaminolyticus was purified and subjected to FTIR and NMR spectral analysis which gave evidence of the active characteristics of the surfactant. To augment the an...

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Autores principales:	Suganya Kannan, Anitta Solomon, Govindan Krishnamoorthy, Murugan Marudhamuthu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2d93bb7025c49d9bb570f2ff5601e75
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spelling	oai:doaj.org-article:a2d93bb7025c49d9bb570f2ff5601e752021-12-02T15:23:06ZLiposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA10.1038/s41598-020-79976-72045-2322https://doaj.org/article/a2d93bb7025c49d9bb570f2ff5601e752021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79976-7https://doaj.org/toc/2045-2322Abstract In the present study lipopeptide biosurfactant with high emulsification capacity produced by human skin bacterium Paenibacillus thiaminolyticus was purified and subjected to FTIR and NMR spectral analysis which gave evidence of the active characteristics of the surfactant. To augment the antivirulent potential further, the mixer of copper and copper oxide nanoparticles (CuNPs) was synthesized, and characterized by UV–Visible spectroscopy, SEM-EDAX, TEM, and Zeta analysis. Here, we attempted to enhance the antimicrobial and antibiofilm activity with the assistance of encapsulated preparation of lipopeptide and CuNPs in multilamellar liposomes. The proposed mechanism of action of lipopeptide and CuNPs liposomal preparation negatively influences the cell metabolism, secreted virulence such as staphyloxanthin, pyocyanin, and extracellular polysaccharides. The significant decline in the growth of MRSA and P. aeruginosa in both planktonic form and biofilm by lipopeptide and CuNPs treatment were visualized using scanning electron microscopy and High content screening imaging system. In vivo studies revealed that treatment with lipopeptide and CuNPs in multilamellar liposomes extended the lifespan of infected Caenorhabditis elegans by about 75%. Therefore, this study typifies lipopeptide and CuNPs could credibly be a substantial substitute over conventional antibiotics in averting the biofilm associated pathogenesis of MRSA and P. aeruginosa.Suganya KannanAnitta SolomonGovindan KrishnamoorthyMurugan MarudhamuthuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Suganya Kannan Anitta Solomon Govindan Krishnamoorthy Murugan Marudhamuthu Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
description	Abstract In the present study lipopeptide biosurfactant with high emulsification capacity produced by human skin bacterium Paenibacillus thiaminolyticus was purified and subjected to FTIR and NMR spectral analysis which gave evidence of the active characteristics of the surfactant. To augment the antivirulent potential further, the mixer of copper and copper oxide nanoparticles (CuNPs) was synthesized, and characterized by UV–Visible spectroscopy, SEM-EDAX, TEM, and Zeta analysis. Here, we attempted to enhance the antimicrobial and antibiofilm activity with the assistance of encapsulated preparation of lipopeptide and CuNPs in multilamellar liposomes. The proposed mechanism of action of lipopeptide and CuNPs liposomal preparation negatively influences the cell metabolism, secreted virulence such as staphyloxanthin, pyocyanin, and extracellular polysaccharides. The significant decline in the growth of MRSA and P. aeruginosa in both planktonic form and biofilm by lipopeptide and CuNPs treatment were visualized using scanning electron microscopy and High content screening imaging system. In vivo studies revealed that treatment with lipopeptide and CuNPs in multilamellar liposomes extended the lifespan of infected Caenorhabditis elegans by about 75%. Therefore, this study typifies lipopeptide and CuNPs could credibly be a substantial substitute over conventional antibiotics in averting the biofilm associated pathogenesis of MRSA and P. aeruginosa.
format	article
author	Suganya Kannan Anitta Solomon Govindan Krishnamoorthy Murugan Marudhamuthu
author_facet	Suganya Kannan Anitta Solomon Govindan Krishnamoorthy Murugan Marudhamuthu
author_sort	Suganya Kannan
title	Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
title_short	Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
title_full	Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
title_fullStr	Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
title_full_unstemmed	Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
title_sort	liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic pseudomonas aeruginosa and mrsa
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a2d93bb7025c49d9bb570f2ff5601e75
work_keys_str_mv	AT suganyakannan liposomeencapsulatedsurfactantabettedcoppernanoparticlesalleviatesbiofilmmediatedvirulenceinpathogenicpseudomonasaeruginosaandmrsa AT anittasolomon liposomeencapsulatedsurfactantabettedcoppernanoparticlesalleviatesbiofilmmediatedvirulenceinpathogenicpseudomonasaeruginosaandmrsa AT govindankrishnamoorthy liposomeencapsulatedsurfactantabettedcoppernanoparticlesalleviatesbiofilmmediatedvirulenceinpathogenicpseudomonasaeruginosaandmrsa AT muruganmarudhamuthu liposomeencapsulatedsurfactantabettedcoppernanoparticlesalleviatesbiofilmmediatedvirulenceinpathogenicpseudomonasaeruginosaandmrsa
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Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA

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