Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA

Abstract In the present study lipopeptide biosurfactant with high emulsification capacity produced by human skin bacterium Paenibacillus thiaminolyticus was purified and subjected to FTIR and NMR spectral analysis which gave evidence of the active characteristics of the surfactant. To augment the an...

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Autores principales: Suganya Kannan, Anitta Solomon, Govindan Krishnamoorthy, Murugan Marudhamuthu
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:a2d93bb7025c49d9bb570f2ff5601e752021-12-02T15:23:06ZLiposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA10.1038/s41598-020-79976-72045-2322https://doaj.org/article/a2d93bb7025c49d9bb570f2ff5601e752021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79976-7https://doaj.org/toc/2045-2322Abstract In the present study lipopeptide biosurfactant with high emulsification capacity produced by human skin bacterium Paenibacillus thiaminolyticus was purified and subjected to FTIR and NMR spectral analysis which gave evidence of the active characteristics of the surfactant. To augment the antivirulent potential further, the mixer of copper and copper oxide nanoparticles (CuNPs) was synthesized, and characterized by UV–Visible spectroscopy, SEM-EDAX, TEM, and Zeta analysis. Here, we attempted to enhance the antimicrobial and antibiofilm activity with the assistance of encapsulated preparation of lipopeptide and CuNPs in multilamellar liposomes. The proposed mechanism of action of lipopeptide and CuNPs liposomal preparation negatively influences the cell metabolism, secreted virulence such as staphyloxanthin, pyocyanin, and extracellular polysaccharides. The significant decline in the growth of MRSA and P. aeruginosa in both planktonic form and biofilm by lipopeptide and CuNPs treatment were visualized using scanning electron microscopy and High content screening imaging system. In vivo studies revealed that treatment with lipopeptide and CuNPs in multilamellar liposomes extended the lifespan of infected Caenorhabditis elegans by about 75%. Therefore, this study typifies lipopeptide and CuNPs could credibly be a substantial substitute over conventional antibiotics in averting the biofilm associated pathogenesis of MRSA and P. aeruginosa.Suganya KannanAnitta SolomonGovindan KrishnamoorthyMurugan MarudhamuthuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Suganya Kannan
Anitta Solomon
Govindan Krishnamoorthy
Murugan Marudhamuthu
Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
description Abstract In the present study lipopeptide biosurfactant with high emulsification capacity produced by human skin bacterium Paenibacillus thiaminolyticus was purified and subjected to FTIR and NMR spectral analysis which gave evidence of the active characteristics of the surfactant. To augment the antivirulent potential further, the mixer of copper and copper oxide nanoparticles (CuNPs) was synthesized, and characterized by UV–Visible spectroscopy, SEM-EDAX, TEM, and Zeta analysis. Here, we attempted to enhance the antimicrobial and antibiofilm activity with the assistance of encapsulated preparation of lipopeptide and CuNPs in multilamellar liposomes. The proposed mechanism of action of lipopeptide and CuNPs liposomal preparation negatively influences the cell metabolism, secreted virulence such as staphyloxanthin, pyocyanin, and extracellular polysaccharides. The significant decline in the growth of MRSA and P. aeruginosa in both planktonic form and biofilm by lipopeptide and CuNPs treatment were visualized using scanning electron microscopy and High content screening imaging system. In vivo studies revealed that treatment with lipopeptide and CuNPs in multilamellar liposomes extended the lifespan of infected Caenorhabditis elegans by about 75%. Therefore, this study typifies lipopeptide and CuNPs could credibly be a substantial substitute over conventional antibiotics in averting the biofilm associated pathogenesis of MRSA and P. aeruginosa.
format article
author Suganya Kannan
Anitta Solomon
Govindan Krishnamoorthy
Murugan Marudhamuthu
author_facet Suganya Kannan
Anitta Solomon
Govindan Krishnamoorthy
Murugan Marudhamuthu
author_sort Suganya Kannan
title Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
title_short Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
title_full Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
title_fullStr Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
title_full_unstemmed Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA
title_sort liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic pseudomonas aeruginosa and mrsa
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/a2d93bb7025c49d9bb570f2ff5601e75
work_keys_str_mv AT suganyakannan liposomeencapsulatedsurfactantabettedcoppernanoparticlesalleviatesbiofilmmediatedvirulenceinpathogenicpseudomonasaeruginosaandmrsa
AT anittasolomon liposomeencapsulatedsurfactantabettedcoppernanoparticlesalleviatesbiofilmmediatedvirulenceinpathogenicpseudomonasaeruginosaandmrsa
AT govindankrishnamoorthy liposomeencapsulatedsurfactantabettedcoppernanoparticlesalleviatesbiofilmmediatedvirulenceinpathogenicpseudomonasaeruginosaandmrsa
AT muruganmarudhamuthu liposomeencapsulatedsurfactantabettedcoppernanoparticlesalleviatesbiofilmmediatedvirulenceinpathogenicpseudomonasaeruginosaandmrsa
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